Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species
The nuclear ribosomal DNA of Rhizoctonia species is polymorphic in terms of the nucleotide composition and length. Insertions of 349e410 nucleotides in length with characteristics of group I introns were detected at a single insertion point at the expansion segment D11 of 28S rDNA in 12 out of 64 is...
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| Formaat: | info:eu-repo/semantics/article |
| Taal: | eng |
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2013
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| Online toegang: | http://inecol.repositorioinstitucional.mx/jspui/handle/1005/7 |
| _version_ | 1799772262439583744 |
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| author | MARIA DOLORES GONZALEZ HERNANDEZ |
| author_facet | MARIA DOLORES GONZALEZ HERNANDEZ |
| author_sort | MARIA DOLORES GONZALEZ HERNANDEZ |
| collection | REPO |
| description | The nuclear ribosomal DNA of Rhizoctonia species is polymorphic in terms of the nucleotide composition and length. Insertions of 349e410 nucleotides in length with characteristics of group I introns were detected at a single insertion point at the expansion segment D11 of 28S rDNA in 12 out of 64 isolates. Eleven corresponded to Rhizoctonia solani (teleomorph: Thanatephorous) and one (AG-Q) to Rhizoctonia spp. (teleomorph: Ceratobasidium). Sequence data showed that all but AG-Q contained conserved DNA catalytic core regions (P, Q, R, and S) essential for selfsplicing. |
| format | info:eu-repo/semantics/article |
| id | repo-1005-7 |
| institution | Repositorio INECOL |
| language | eng |
| publishDate | 2013 |
| record_format | dspace |
| spelling | repo-1005-72016-09-07T19:41:26Z Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species MARIA DOLORES GONZALEZ HERNANDEZ 2013 info:eu-repo/semantics/article The nuclear ribosomal DNA of Rhizoctonia species is polymorphic in terms of the nucleotide composition and length. Insertions of 349e410 nucleotides in length with characteristics of group I introns were detected at a single insertion point at the expansion segment D11 of 28S rDNA in 12 out of 64 isolates. Eleven corresponded to Rhizoctonia solani (teleomorph: Thanatephorous) and one (AG-Q) to Rhizoctonia spp. (teleomorph: Ceratobasidium). Sequence data showed that all but AG-Q contained conserved DNA catalytic core regions (P, Q, R, and S) essential for selfsplicing. The predicted secondary structure revealed that base- paired helices corresponded to subgroup IC1. Isolates from same anastomosis group and even subgroups within R. solani were variable with regard to possession of introns. Phylo- genetic analyses indicated that introns were vertically transmitted. Unfortunately, sequence data from the conserved region from all 64 isolates were not useful for delimiting species. Analyses with IC1 introns at same insertion point, of both Ascomycota and Basidio- mycota indicated the possibility of horizontal transfer at this site. The present study uncov- ered new questions on evolutionary pattern of change of these introns within Rhizoctonia species. info:eu-repo/classification/AUTOR/Anamorph, LSU rDNA, Phytopathogenic fungi, RNA secondary structure, Thanatephorus info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 http://inecol.repositorioinstitucional.mx/jspui/handle/1005/7 info:eu-repo/semantics/openAccess eng citation:González Hernández M.D. 2013. Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species. Fungal Biology. 117: 623-637 http://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf |
| spellingShingle | info:eu-repo/classification/AUTOR/Anamorph, LSU rDNA, Phytopathogenic fungi, RNA secondary structure, Thanatephorus info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 MARIA DOLORES GONZALEZ HERNANDEZ Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species |
| title | Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species |
| title_full | Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species |
| title_fullStr | Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species |
| title_full_unstemmed | Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species |
| title_short | Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species |
| title_sort | identification molecular characterization and evolution of group i introns at the expansion segment d11 of 28s rdna inrhizoctonia species |
| topic | info:eu-repo/classification/AUTOR/Anamorph, LSU rDNA, Phytopathogenic fungi, RNA secondary structure, Thanatephorus info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 |
| url | http://inecol.repositorioinstitucional.mx/jspui/handle/1005/7 |
| work_keys_str_mv | AT mariadoloresgonzalezhernandez identificationmolecularcharacterizationandevolutionofgroupiintronsattheexpansionsegmentd11of28srdnainrhizoctoniaspecies |
