Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts
Background and Aims: Cultivation of Ganoderma on vineyard pruning wood chips improves the productivity and biochemical composition of their fruiting bodies. Consequently, it has gained traction as an alternative for recycling viticulture waste. The current trend is to increase the production of Gano...
| Principais autores: | , , , , , , |
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| Formato: | Online |
| Idioma: | eng |
| Publicado em: |
Instituto de Ecología, A.C.
2024
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| Acesso em linha: | https://abm.ojs.inecol.mx/index.php/abm/article/view/2258 |
| _version_ | 1799771439529721856 |
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| author | Cruz-Félix, María Angulo-Sanchez, Lucia Vargas, Georgina Gutiérrez , Aldo Orozco , Antonio Ramos-Clamont , Gabriela Esqueda, Martín |
| author_facet | Cruz-Félix, María Angulo-Sanchez, Lucia Vargas, Georgina Gutiérrez , Aldo Orozco , Antonio Ramos-Clamont , Gabriela Esqueda, Martín |
| author_sort | Cruz-Félix, María |
| collection | ABM |
| description | Background and Aims: Cultivation of Ganoderma on vineyard pruning wood chips improves the productivity and biochemical composition of their fruiting bodies. Consequently, it has gained traction as an alternative for recycling viticulture waste. The current trend is to increase the production of Ganoderma bioactive compounds in liquid culture. This work aims to evaluate the effect of vineyard pruning extracts on biomass in the liquid culture of Ganoderma native strains from the Sonoran desert, Mexico. Total triterpenoid and antioxidant capacity were prospected in the most competitive native strain vs. control strain.
Methods: Vineyard pruning extracts of different polarity and concentrations were evaluated in a cornmeal-glucose-peptone liquid culture to grow Ganoderma spp. for seven days at 25 °C. The antioxidant capacity was determined with ORAC, FRAP, and TEAC assays. The total triterpenoid content was estimated using the spectrophotometric method. The structural analysis was determined by FTIR.
Results: The highest overall biomass production was observed in Ganoderma oerstedii, followed by G. subincrustatum, G. weberianum, and G. lucidum (control strain). The highest biomass production was obtained with the polar extract at 500 µg/l. The culture conditions produced a variable response in the antioxidant capacity. The highest total triterpenoid content (686±21 µg/ml) was observed in G. oerstedii at nine days of culture. FTIR analysis showed the presence of hydroxyl and enol groups, and benzene rings characteristic of triterpenoids.
Conclusions: Response surface analysis revealed that vineyard pruning extracts enhanced biomass production of Ganoderma spp. in liquid culture. The enhancement varied based on the species, polarity, and concentration of the extract. The antioxidant capacity and total triterpenoid content exhibit variations according to time and culture conditions. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-2258 |
| institution | Acta Botanica Mexicana |
| language | eng |
| publishDate | 2024 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-22582024-03-07T03:30:53Z Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts Potenciación de producción de biomasa de cepas de Ganoderma spp. (Polyporaceae) nativas del desierto Sonorense, México, crecidas en cultivo líquido con extractos de madera de vid Cruz-Félix, María Angulo-Sanchez, Lucia Vargas, Georgina Gutiérrez , Aldo Orozco , Antonio Ramos-Clamont , Gabriela Esqueda, Martín cultivo líquido hongo medicinal reishi triterpenoides. liquid culture medicinal mushroom reishi triterpenoids. Background and Aims: Cultivation of Ganoderma on vineyard pruning wood chips improves the productivity and biochemical composition of their fruiting bodies. Consequently, it has gained traction as an alternative for recycling viticulture waste. The current trend is to increase the production of Ganoderma bioactive compounds in liquid culture. This work aims to evaluate the effect of vineyard pruning extracts on biomass in the liquid culture of Ganoderma native strains from the Sonoran desert, Mexico. Total triterpenoid and antioxidant capacity were prospected in the most competitive native strain vs. control strain. Methods: Vineyard pruning extracts of different polarity and concentrations were evaluated in a cornmeal-glucose-peptone liquid culture to grow Ganoderma spp. for seven days at 25 °C. The antioxidant capacity was determined with ORAC, FRAP, and TEAC assays. The total triterpenoid content was estimated using the spectrophotometric method. The structural analysis was determined by FTIR. Results: The highest overall biomass production was observed in Ganoderma oerstedii, followed by G. subincrustatum, G. weberianum, and G. lucidum (control strain). The highest biomass production was obtained with the polar extract at 500 µg/l. The culture conditions produced a variable response in the antioxidant capacity. The highest total triterpenoid content (686±21 µg/ml) was observed in G. oerstedii at nine days of culture. FTIR analysis showed the presence of hydroxyl and enol groups, and benzene rings characteristic of triterpenoids. Conclusions: Response surface analysis revealed that vineyard pruning extracts enhanced biomass production of Ganoderma spp. in liquid culture. The enhancement varied based on the species, polarity, and concentration of the extract. The antioxidant capacity and total triterpenoid content exhibit variations according to time and culture conditions. Antecedentes y Objetivos: El cultivo de Ganoderma en madera de vid mejora la productividad y composición bioquímica de sus cuerpos fructíferos, por lo que ha ganado fuerza como alternativa para el reciclaje de residuos de viticultura. Sin embargo, la tendencia actual es incrementar los compuestos bioactivos de Ganoderma en cultivo líquido. Este trabajo tiene como objetivo evaluar el efecto de extractos de madera de vid sobre la producción de biomasa en cultivo líquido de cepas de Ganoderma nativas del desierto Sonorense. Triterpenoides totales y capacidad antioxidante se exploraron en la cepa nativa más competitiva vs. control. Métodos: Se evaluaron extractos de madera de vid de diferente polaridad y concentraciones en un cultivo líquido de harina de maíz-glucosa-peptona con Ganoderma spp. durante siete días a 25 °C. La capacidad antioxidante se determinó con los ensayos de ORAC, FRAP y TEAC. El contenido total de triterpenoides se estimó usando el método espectrofotométrico, y el análisis estructural se determinó por FTIR. Resultados: La mayor producción total de biomasa se observó en Ganoderma oerstedii, seguida de G. subincrustatum, G. weberianum y G. lucidum (cepa control). La producción de biomasa más alta se obtuvo con el extracto polar a 500 µg/l. Las condiciones de cultivo produjeron una respuesta variable en la capacidad antioxidante. El mayor contenido de triterpenoides totales (686±21 µg/ml) se observó en G. oerstedii a los nueve días de cultivo. El análisis de FTIR mostró la presencia de grupos hidroxilo y enol, y anillos de benceno característicos de triterpenos. Conclusión: El análisis de superficie de respuesta reveló que los extractos de madera de vid mejoraron la producción de biomasa de Ganoderma spp. en cultivo líquido. La potenciación varió según la especie, la polaridad y la concentración del extracto. La capacidad antioxidante y el contenido de triterpenoides totales presentan variaciones según el tiempo y las condiciones de cultivo. Instituto de Ecología, A.C. 2024-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/2258 10.21829/abm131.2024.2258 Acta Botanica Mexicana; No. 131 (2024) Acta Botanica Mexicana; Núm. 131 (2024) 2448-7589 0187-7151 eng https://abm.ojs.inecol.mx/index.php/abm/article/view/2258/4507 https://abm.ojs.inecol.mx/index.php/abm/article/view/2258/4508 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Cruz-Félix, María Angulo-Sanchez, Lucia Vargas, Georgina Gutiérrez , Aldo Orozco , Antonio Ramos-Clamont , Gabriela Esqueda, Martín Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts |
| title | Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts |
| title_full | Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts |
| title_fullStr | Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts |
| title_full_unstemmed | Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts |
| title_short | Enhancement of biomass production of Ganoderma spp. (Polyporaceae) native strains from the Sonoran desert, Mexico, grown in liquid culture with vineyard pruning extracts |
| title_sort | enhancement of biomass production of ganoderma spp. (polyporaceae) native strains from the sonoran desert, mexico, grown in liquid culture with vineyard pruning extracts |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/2258 |
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