Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae)
Background and Aims: Bursera simaruba leaves are traditionally used to treat various illnesses. Nonetheless, there are few reports on the description of the phytochemicals potentially responsible for such biological activities. Therefore, this study aimed to describe the antifungal, antibacterial, a...
| Autors principals: | , , , , , , , , , |
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| Format: | Online |
| Idioma: | eng spa |
| Publicat: |
Instituto de Ecología, A.C.
2022
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| Accés en línia: | https://abm.ojs.inecol.mx/index.php/abm/article/view/2109 |
| _version_ | 1799771421577052160 |
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| author | Infante-Rodríguez, Dennis Adrián Landa-Cansigno, Cristina Gutiérrez-Sánchez, Angélica Murrieta-León, Diana Lizzet Reyes-López, Celeste Castillejos-Pérez, Adriana Belén Pucheta-Fiscal, José Efrain Velázquez-Narváez, Alberto Carlos Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio |
| author_facet | Infante-Rodríguez, Dennis Adrián Landa-Cansigno, Cristina Gutiérrez-Sánchez, Angélica Murrieta-León, Diana Lizzet Reyes-López, Celeste Castillejos-Pérez, Adriana Belén Pucheta-Fiscal, José Efrain Velázquez-Narváez, Alberto Carlos Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio |
| author_sort | Infante-Rodríguez, Dennis Adrián |
| collection | ABM |
| description | Background and Aims: Bursera simaruba leaves are traditionally used to treat various illnesses. Nonetheless, there are few reports on the description of the phytochemicals potentially responsible for such biological activities. Therefore, this study aimed to describe the antifungal, antibacterial, and antidiabetic potential by using in vitro experiments, and to contribute to the knowledge of the chemical composition of B. simaruba leaves.
Methods: A methanolic extract (MeOH-Ex) of B. simaruba leaves was tested for antibacterial, antifungal, and antidiabetic activities, and different groups of secondary metabolites were detected by qualitative assays. Furthermore, phytochemical analysis of MeOH-Ex was carried out by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI+-MS-QTOF), and putative identifications were performed using public spectral databases.
Key results: The MeOH-Ex of the leaves of B. simaruba qualitatively contains alkaloids, terpenes and steroids, saponins, tannins, coumarins and phenolic compounds, such as caffeic acid, chlorogenic acid, apigenin, kaempferol, phlorizin, quercitrin, quercetin-glucoside and apigenin-glycoside. In addition, burseran and yatein lignans were tentatively identified. MeOH-Ex exhibited low antifungal activity against Fusarium solani (16.3% mycelial growth inhibition) and a high antidiabetic effect by in vitro inhibition of α-amylase (87.7%) and α-glucosidase (75.9%) enzymes. Finally, chlorogenic acid standard exhibited a significant inhibition of α-amylase (49.5%) and α-glucosidase (85.1%) enzymes.
Conclusions: The MeOH-Ex of B. simaruba leaves represents a source of secondary metabolites with potential antidiabetic activity. The phenolic compounds tentatively identified could play important roles in preventing disorders due to post-prandial hyperglycemia by inhibiting the enzymes α-amylase and α-glucosidase. Chlorogenic acid presence is highlighted as one of the main potential bioactive compounds in B. simaruba leaves. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-2109 |
| institution | Acta Botanica Mexicana |
| language | eng spa |
| publishDate | 2022 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-21092023-03-17T06:05:07Z Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) Análisis fitoquímico y actividad antidiabética, antibacteriana y antifúngica de hojas de Bursera simaruba (Burseraceae) Infante-Rodríguez, Dennis Adrián Landa-Cansigno, Cristina Gutiérrez-Sánchez, Angélica Murrieta-León, Diana Lizzet Reyes-López, Celeste Castillejos-Pérez, Adriana Belén Pucheta-Fiscal, José Efrain Velázquez-Narváez, Alberto Carlos Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio compuestos fenólicos enzima α-amilasa enzima α-glucosidasa espectrometría de masas metabolitos bioactivos de plantas. α-amylase enzyme α-glucosidase enzyme bioactive plant metabolites mass spectrometry phenolic compounds. Background and Aims: Bursera simaruba leaves are traditionally used to treat various illnesses. Nonetheless, there are few reports on the description of the phytochemicals potentially responsible for such biological activities. Therefore, this study aimed to describe the antifungal, antibacterial, and antidiabetic potential by using in vitro experiments, and to contribute to the knowledge of the chemical composition of B. simaruba leaves. Methods: A methanolic extract (MeOH-Ex) of B. simaruba leaves was tested for antibacterial, antifungal, and antidiabetic activities, and different groups of secondary metabolites were detected by qualitative assays. Furthermore, phytochemical analysis of MeOH-Ex was carried out by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI+-MS-QTOF), and putative identifications were performed using public spectral databases. Key results: The MeOH-Ex of the leaves of B. simaruba qualitatively contains alkaloids, terpenes and steroids, saponins, tannins, coumarins and phenolic compounds, such as caffeic acid, chlorogenic acid, apigenin, kaempferol, phlorizin, quercitrin, quercetin-glucoside and apigenin-glycoside. In addition, burseran and yatein lignans were tentatively identified. MeOH-Ex exhibited low antifungal activity against Fusarium solani (16.3% mycelial growth inhibition) and a high antidiabetic effect by in vitro inhibition of α-amylase (87.7%) and α-glucosidase (75.9%) enzymes. Finally, chlorogenic acid standard exhibited a significant inhibition of α-amylase (49.5%) and α-glucosidase (85.1%) enzymes. Conclusions: The MeOH-Ex of B. simaruba leaves represents a source of secondary metabolites with potential antidiabetic activity. The phenolic compounds tentatively identified could play important roles in preventing disorders due to post-prandial hyperglycemia by inhibiting the enzymes α-amylase and α-glucosidase. Chlorogenic acid presence is highlighted as one of the main potential bioactive compounds in B. simaruba leaves. Antecedentes y Objetivos: Las hojas de Bursera simaruba se utilizan tradicionalmente para tratar diversas enfermedades. Sin embargo, existen pocos reportes sobre la descripción de los fitoquímicos potencialmente responsables de tales actividades biológicas. Por lo tanto, este estudio tuvo como objetivo describir el potencial antifúngico, antibacteriano y antidiabético mediante experimentos in vitro, así como contribuir al conocimiento de la composición química de las hojas de B. simaruba. Métodos: Se analizó la actividad antibacteriana, antifúngica y antidiabética de un extracto metanólico (MeOH-Ex) de hojas de B. simaruba, y se detectaron diferentes grupos de metabolitos secundarios mediante ensayos cualitativos. Además, el análisis fitoquímico de MeOH-Ex se determinó con cromatografía líquida de ultra alta resolución acoplada a espectrometría de masas de alta resolución (UHPLC-ESI+-MS-QTOF), y las identificaciones putativas se realizaron utilizando bases de datos espectrales públicas. Resultados clave: El MeOH-Ex de las hojas de B. simaruba contiene cualitativamente alcaloides, terpenos y esteroides, saponinas, taninos, cumarinas y compuestos fenólicos, tales como ácido cafeico, ácido clorogénico, apigenina, kaempferol, florizina, quercitrina, quercetina-glucósido y apigenina-glucósido. Además, se identificaron tentativamente los lignanos burseran y yatein. El MeOH-Ex exhibió una baja actividad antifúngica contra Fusarium solani (16.3% de inhibición del crecimiento micelial) y un alto efecto antidiabético por inhibición in vitro de las enzimas α-amilasa (87.7%) y α-glucosidasa (75.9%). Finalmente, el estándar de ácido clorogénico mostró una inhibición significativa de las enzimas α-amilasa (49.5%) y α-glucosidasa (85.1%). Conclusiones: El MeOH-Ex de las hojas de B. simaruba representa una fuente de metabolitos secundarios con potencial actividad antidiabética. Los compuestos fenólicos tentativamente identificados podrían desempeñar un papel importante en la prevención de trastornos por hiperglucemia posprandial al inhibir las enzimas α-amilasa y α-glucosidasa. Destaca la presencia de ácido clorogénico como uno de los principales compuestos bioactivos potenciales en las hojas de B. simaruba. Instituto de Ecología, A.C. 2022-12-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/2109 10.21829/abm129.2022.2109 Acta Botanica Mexicana; No. 129 (2022) Acta Botanica Mexicana; Núm. 129 (2022) 2448-7589 0187-7151 eng spa https://abm.ojs.inecol.mx/index.php/abm/article/view/2109/4330 https://abm.ojs.inecol.mx/index.php/abm/article/view/2109/4378 https://abm.ojs.inecol.mx/index.php/abm/article/view/2109/4331 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Infante-Rodríguez, Dennis Adrián Landa-Cansigno, Cristina Gutiérrez-Sánchez, Angélica Murrieta-León, Diana Lizzet Reyes-López, Celeste Castillejos-Pérez, Adriana Belén Pucheta-Fiscal, José Efrain Velázquez-Narváez, Alberto Carlos Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) |
| title | Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) |
| title_full | Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) |
| title_fullStr | Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) |
| title_full_unstemmed | Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) |
| title_short | Phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of Bursera simaruba (Burseraceae) |
| title_sort | phytochemical analysis and antidiabetic, antibacterial, and antifungal activities of leaves of bursera simaruba (burseraceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/2109 |
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