Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria
Background and Aims: Conifers, such as pines, establish obligate symbioses with ectomycorrhizal fungi and specialized bacteria in their roots. Mexico has a high diversity of species of the genus Pinus, represented by 72 taxa in this country. However, currently the research related to the coinoculati...
| Príomhchruthaitheoirí: | , , , , , , |
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| Formáid: | Online |
| Teanga: | spa |
| Foilsithe / Cruthaithe: |
Instituto de Ecología, A.C.
2022
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| Rochtain ar líne: | https://abm.ojs.inecol.mx/index.php/abm/article/view/2024 |
| _version_ | 1799771415297130496 |
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| author | Barragán-Soriano, José Luis Pérez-Moreno, Jesús Almaraz-Suárez, Juan José Carcaño-Montiel , Moisés Graciano Delgadillo-Martínez , Julián Cetina-Alcalá , Víctor Manuel Mata , Gerardo |
| author_facet | Barragán-Soriano, José Luis Pérez-Moreno, Jesús Almaraz-Suárez, Juan José Carcaño-Montiel , Moisés Graciano Delgadillo-Martínez , Julián Cetina-Alcalá , Víctor Manuel Mata , Gerardo |
| author_sort | Barragán-Soriano, José Luis |
| collection | ABM |
| description | Background and Aims: Conifers, such as pines, establish obligate symbioses with ectomycorrhizal fungi and specialized bacteria in their roots. Mexico has a high diversity of species of the genus Pinus, represented by 72 taxa in this country. However, currently the research related to the coinoculation of Mexican pines with ectomycorrhizal fungi and beneficial bacteria has received little attention. In this work, the effect of coinoculation with an edible ectomycorrhizal fungus and three bacterial strains was evaluated in terms of growth, physiological and nutritional variables in plants of Pinus montezumae.
Methods: Pinus montezumae plants were inoculated and coinoculated with the edible ectomycorrhizal fungus Laccaria laccata, and with three identified bacterial strains: one as Cohnella sp., and two as Azospirillum brasilense (one able to fix nitrogen and the other one able to solubilize phosphates). This research lasted 520 days and was carried out in a greenhouse of the Colegio de Postgraduados, Campus Montecillo, Texcoco, Mexico State, Mexico.
Key results: Inoculation with the ectomycorrhizal fungus led to an increase in terms of dry weight of up to 7.8 times and photosynthetic rate up to 30 times higher compared with plants without any inoculation. Additionally, a synergistic effect was recorded in terms of shoot and total growth, stem diameter, photosynthetic rate, total chlorophyll and carotene content, as well as nitrogen and potassium content of the mycorrhized plants coinoculated with the ectomycorrhizal fungus and the bacterium A. brasilense able to fix nitrogen.
Conclusions: This work demonstrates the importance of the coinoculation of an edible ectomycorrhizal fungus and the bacterium A. brasilense, in terms of growth, physiology and nutritional content enhancement of P. montezumae, a pine species of great forest importance in Mexico. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-2024 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2022 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-20242022-07-14T09:07:35Z Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria Coinoculación de Pinus montezumae (Pinaceae) con un hongo comestible ectomicorrízico y bacterias promotoras de crecimiento vegetal Barragán-Soriano, José Luis Pérez-Moreno, Jesús Almaraz-Suárez, Juan José Carcaño-Montiel , Moisés Graciano Delgadillo-Martínez , Julián Cetina-Alcalá , Víctor Manuel Mata , Gerardo bacteria biotechnology ectomycorrhizal symbiosis edible ectomycorrhizal fungi pines. bacterias biotecnología hongos comestibles ectomicorrízicos pinos simbiosis ectomicorrízica. Background and Aims: Conifers, such as pines, establish obligate symbioses with ectomycorrhizal fungi and specialized bacteria in their roots. Mexico has a high diversity of species of the genus Pinus, represented by 72 taxa in this country. However, currently the research related to the coinoculation of Mexican pines with ectomycorrhizal fungi and beneficial bacteria has received little attention. In this work, the effect of coinoculation with an edible ectomycorrhizal fungus and three bacterial strains was evaluated in terms of growth, physiological and nutritional variables in plants of Pinus montezumae. Methods: Pinus montezumae plants were inoculated and coinoculated with the edible ectomycorrhizal fungus Laccaria laccata, and with three identified bacterial strains: one as Cohnella sp., and two as Azospirillum brasilense (one able to fix nitrogen and the other one able to solubilize phosphates). This research lasted 520 days and was carried out in a greenhouse of the Colegio de Postgraduados, Campus Montecillo, Texcoco, Mexico State, Mexico. Key results: Inoculation with the ectomycorrhizal fungus led to an increase in terms of dry weight of up to 7.8 times and photosynthetic rate up to 30 times higher compared with plants without any inoculation. Additionally, a synergistic effect was recorded in terms of shoot and total growth, stem diameter, photosynthetic rate, total chlorophyll and carotene content, as well as nitrogen and potassium content of the mycorrhized plants coinoculated with the ectomycorrhizal fungus and the bacterium A. brasilense able to fix nitrogen. Conclusions: This work demonstrates the importance of the coinoculation of an edible ectomycorrhizal fungus and the bacterium A. brasilense, in terms of growth, physiology and nutritional content enhancement of P. montezumae, a pine species of great forest importance in Mexico. Antecedentes y Objetivos: Las coníferas, como los pinos, establecen en sus raíces simbiosis obligadas con hongos ectomicorrízicos y con determinados grupos de bacterias. México posee una alta diversidad de especies del género Pinus, que en este país está representado por 72 taxa. Sin embargo, actualmente son escasas las investigaciones relacionadas con la coinoculación de pinos mexicanos con hongos ectomicorrízicos y bacterias benéficas. En este trabajo se evaluó el efecto de la coinoculación, con un hongo comestible ectomicorrízico y tres cepas bacterianas, en términos de variables de crecimiento, fisiológicas y nutrimentales en plantas de Pinus montezumae. Métodos: Se inocularon y coinocularon plantas de P. montezumae con el hongo comestible ectomicorrízico Laccaria laccata, y con tres cepas bacterianas, una de Cohnella sp., y dos de Azospirillum brasilense (una con capacidad de fijar nitrógeno y la otra con capacidad de solubilizar fosfatos). Esta investigación duró 520 días y se realizó en un invernadero del Colegio de Postgraduados, Campus Montecillo, Texcoco, Estado de México, México. Resultados clave: La inoculación con el hongo ectomicorrízico produjo incrementos en términos de peso seco de hasta 7.8 veces y tasas fotosintéticas hasta 30 veces mayores comparadas con las plantas sin inocular. Adicionalmente se registró un efecto sinérgico en términos del crecimiento de parte área y total, diámetro del tallo, tasa fotosintética, contenido de clorofilas totales y carotenos, así como contenido de nitrógeno y potasio de las plantas micorrizadas coinoculadas con el hongo ectomicorrízico y la bacteria A. brasilense fijadora de nitrógeno. Conclusiones: Este trabajo demuestra la importancia de la coinoculación de un hongo ectomicorrízico comestible y la bacteria A. brasilense, en el mejoramiento del crecimiento, fisiología y contenido nutrimental de P. montezumae, una especie de pino de gran importancia forestal en México. Instituto de Ecología, A.C. 2022-05-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/2024 10.21829/abm129.2022.2024 Acta Botanica Mexicana; No. 129 (2022) Acta Botanica Mexicana; Núm. 129 (2022) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/2024/4206 https://abm.ojs.inecol.mx/index.php/abm/article/view/2024/4237 https://abm.ojs.inecol.mx/index.php/abm/article/view/2024/4207 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Barragán-Soriano, José Luis Pérez-Moreno, Jesús Almaraz-Suárez, Juan José Carcaño-Montiel , Moisés Graciano Delgadillo-Martínez , Julián Cetina-Alcalá , Víctor Manuel Mata , Gerardo Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| title | Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| title_full | Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| title_fullStr | Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| title_full_unstemmed | Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| title_short | Coinoculation of Pinus montezumae (Pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| title_sort | coinoculation of pinus montezumae (pinaceae) with an edible ectomycorrhizal fungus and plant growth promoting bacteria |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/2024 |
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