Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach
Background and Aims: Gelidium americanum is widely distributed on the Atlantic coast of Mexico; however, so far no studies have been conducted to confirm its taxonomic identity using molecular markers and morphological characters. The objective of this study was to confirm the taxonomic identity, wi...
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| Formáid: | Online |
| Teanga: | spa |
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Instituto de Ecología, A.C.
2021
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| Rochtain ar líne: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1966 |
| _version_ | 1799771409608605696 |
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| author | Quiroz-González, Nataly Rivas Acuña, Ma. Guadalupe Ponce Márquez, Ma. Edith |
| author_facet | Quiroz-González, Nataly Rivas Acuña, Ma. Guadalupe Ponce Márquez, Ma. Edith |
| author_sort | Quiroz-González, Nataly |
| collection | ABM |
| description | Background and Aims: Gelidium americanum is widely distributed on the Atlantic coast of Mexico; however, so far no studies have been conducted to confirm its taxonomic identity using molecular markers and morphological characters. The objective of this study was to confirm the taxonomic identity, with a morphological and molecular approach, of the specimens identified as G. americanum previously collected in the coastal lagoon of Mecoacán, Tabasco, Mexico.Methods: Three specimens of G. americanum were collected in the Mecoacán lagoon, Tabasco, Mexico. The material was morphologically analyzed by observations with stereoscopic and optical microscopes. For molecular analysis, DNA was extracted from the samples using the CTAB method; rbcL and COI-5P markers were amplified with the Phire Plant Direct kit (PCR), the sequences were edited in Bioedit and aligned in Clustal W. Next, a Maximum Verisimilitude analysis was done in RaxML, a Bayesian Inference analysis in MrBayes, and genetic distances were calculated in MEGA.Key results: The sequences obtained in the present study for rbcL and COI-5P markers were nested in the clade of samples previously identified with molecular markers as Gelidium americanum. In addition, genetic distances were minimal, the morphological characters coincided with what was previously described for other locations in the Atlantic, despite having a limited number of specimens.Conclusions: Using molecular markers and morphological characters, the presence of Gelidium americanum in the Mecoacán lagoon is confirmed despite having different environmental characteristics to those that have usually been described for this species. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1966 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2021 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-19662022-11-17T00:44:35Z Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach Confirmación taxonómica de Gelidium americanum (Gelidiaceae, Rhodophyta) en Tabasco, México, usando un enfoque morfológico y molecular Quiroz-González, Nataly Rivas Acuña, Ma. Guadalupe Ponce Márquez, Ma. Edith COI-5P Gulf of Mexico morphology phylogeny rbcL red algae algas rojas COI-5P filogenia Golfo de México morfología rbcL Background and Aims: Gelidium americanum is widely distributed on the Atlantic coast of Mexico; however, so far no studies have been conducted to confirm its taxonomic identity using molecular markers and morphological characters. The objective of this study was to confirm the taxonomic identity, with a morphological and molecular approach, of the specimens identified as G. americanum previously collected in the coastal lagoon of Mecoacán, Tabasco, Mexico.Methods: Three specimens of G. americanum were collected in the Mecoacán lagoon, Tabasco, Mexico. The material was morphologically analyzed by observations with stereoscopic and optical microscopes. For molecular analysis, DNA was extracted from the samples using the CTAB method; rbcL and COI-5P markers were amplified with the Phire Plant Direct kit (PCR), the sequences were edited in Bioedit and aligned in Clustal W. Next, a Maximum Verisimilitude analysis was done in RaxML, a Bayesian Inference analysis in MrBayes, and genetic distances were calculated in MEGA.Key results: The sequences obtained in the present study for rbcL and COI-5P markers were nested in the clade of samples previously identified with molecular markers as Gelidium americanum. In addition, genetic distances were minimal, the morphological characters coincided with what was previously described for other locations in the Atlantic, despite having a limited number of specimens.Conclusions: Using molecular markers and morphological characters, the presence of Gelidium americanum in the Mecoacán lagoon is confirmed despite having different environmental characteristics to those that have usually been described for this species. Antecedentes y Objetivos: Gelidium americanum se distribuye ampliamente en la costa Atlántica de México; sin embargo, hasta ahora no se han realizado estudios que confirmen su identidad taxonómica utilizando marcadores moleculares y caracteres morfológicos. El objetivo del presente trabajo fue confirmar la identidad taxonómica con un enfoque morfológico y molecular, de los especímenes identificados como G. americanum recolectados previamente en la laguna costera de Mecoacán, Tabasco, México.Métodos: Se recolectaron tres especímenes de G. americanum en la laguna Mecoacán, Tabasco, México. El material se analizó morfológicamente, mediante observaciones en un microscopio estereoscópico y microscopio óptico. Para el análisis molecular se extrajo ADN de los talos muestreados empleando el método CTAB; se amplificaron los marcadores rbcL y COI-5P con el kit Phire Plant Direct (PCR), las secuencias fueron editadas en Bioedit y alineadas en Clustal W. Enseguida se hizo un análisis de Máxima Verosimilitud en RaxML, otro de Inferencia Bayesiana en MrBayes y se calcularon las distancias genéticas en MEGA. Resultados clave: Las secuencias obtenidas en el presente estudio para los marcadores rbcL y COI-5P se anidaron en el clado de las muestras identificadas previamente con marcadores moleculares como Gelidium americanum. Además, las distancias genéticas fueron mínimas, los caracteres morfológicos coincidieron con lo descrito previamente para otras localidades del Atlántico, a pesar de contar con un limitado número de especímenes.Conclusiones: Utilizando marcadores moleculares y caracteres morfológicos, se confirma la presencia de Gelidium americanum en la laguna Mecoacán a pesar de presentar características ambientales diferentes a las que habitualmente se han descrito para esta especie. Instituto de Ecología, A.C. 2021-12-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/1966 10.21829/abm128.2021.1966 Acta Botanica Mexicana; No. 128 (2021) Acta Botanica Mexicana; Núm. 128 (2021) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1966/3929 https://abm.ojs.inecol.mx/index.php/abm/article/view/1966/3948 https://abm.ojs.inecol.mx/index.php/abm/article/view/1966/3930 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Quiroz-González, Nataly Rivas Acuña, Ma. Guadalupe Ponce Márquez, Ma. Edith Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach |
| title | Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach |
| title_full | Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach |
| title_fullStr | Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach |
| title_full_unstemmed | Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach |
| title_short | Taxonomic confirmation of Gelidium americanum (Gelidiaceae, Rhodophyta) in Tabasco, Mexico, using a morphological and molecular approach |
| title_sort | taxonomic confirmation of gelidium americanum (gelidiaceae, rhodophyta) in tabasco, mexico, using a morphological and molecular approach |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1966 |
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