Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction
Background and Aims: The decrease and fragmentation of populations result in the loss of variation and increase in genetic differentiation. Pinus remota is an arboreal-shrubby species of restricted distribution with scattered and fragmented populations. The objectives of the present study were: to e...
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| Format: | Online |
| Jezik: | spa |
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Instituto de Ecología, A.C.
2021
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| Online pristup: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1890 |
| _version_ | 1799771399448952832 |
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| author | González Jiménez, Aridahí Delgado Valerio, Patricia Molina Sánchez, Agustín Flores López, Celestino Vargas Gutiérrez, Juan Pablo García Magaña, J. Jesús |
| author_facet | González Jiménez, Aridahí Delgado Valerio, Patricia Molina Sánchez, Agustín Flores López, Celestino Vargas Gutiérrez, Juan Pablo García Magaña, J. Jesús |
| author_sort | González Jiménez, Aridahí |
| collection | ABM |
| description | Background and Aims: The decrease and fragmentation of populations result in the loss of variation and increase in genetic differentiation. Pinus remota is an arboreal-shrubby species of restricted distribution with scattered and fragmented populations. The objectives of the present study were: to evaluate the levels of genetic variation, inbreeding and the effective size of the populations, to test the hypothesis of the existence of bottlenecks associated with the reduction of the genetic variation of the populations, and to analyze the genetic structure and its association with the geographic distribution of populations.
Methods: The study was carried out in seven representative populations of their natural distribution in Mexico, by screening a total of 112 trees with five nuclear microsatellites. The data were evaluated with parameters of population genetics and Bayesian approach methods.
Key results: The average expected heterozygosity (He=0.557) was higher than the observed heterozygosity (Ho=0.375). The populations are not in Hardy-Weinberg equilibrium, with significant inbreeding levels (FIS=0.259) and small effective sizes (Ne between 375-425). These are structured in three genetic groups (FST=0.158), with high values of the Mc index (0.186-0.283), suggesting ancestral demographic decline events (between 11,910 to 23,820 years ago), associated with the Pleistocene climate changes.
Conclusions: The study showed that P. remota contains a considerable genetic variation, which is spatially structured and associated with ancestral bottlenecks. Moreover, it is confirmed that P. catarinae is its synonym. The species has been able to survive and adapt to local environmental conditions. With this knowledge conservation strategies for the remaining populations of the species are proposed. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1890 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2021 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-18902022-11-17T00:45:34Z Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction Diversidad genética en poblaciones de Pinus remota (Pinaceae) del Noreste de México: evidencias de declinamiento demográfico histórico González Jiménez, Aridahí Delgado Valerio, Patricia Molina Sánchez, Agustín Flores López, Celestino Vargas Gutiérrez, Juan Pablo García Magaña, J. Jesús bottlenecks conservation genetic structure inbreeding microsatellites Pinus. conservación cuellos de botella endogamia estructura genética microsatélites Pinus. Background and Aims: The decrease and fragmentation of populations result in the loss of variation and increase in genetic differentiation. Pinus remota is an arboreal-shrubby species of restricted distribution with scattered and fragmented populations. The objectives of the present study were: to evaluate the levels of genetic variation, inbreeding and the effective size of the populations, to test the hypothesis of the existence of bottlenecks associated with the reduction of the genetic variation of the populations, and to analyze the genetic structure and its association with the geographic distribution of populations. Methods: The study was carried out in seven representative populations of their natural distribution in Mexico, by screening a total of 112 trees with five nuclear microsatellites. The data were evaluated with parameters of population genetics and Bayesian approach methods. Key results: The average expected heterozygosity (He=0.557) was higher than the observed heterozygosity (Ho=0.375). The populations are not in Hardy-Weinberg equilibrium, with significant inbreeding levels (FIS=0.259) and small effective sizes (Ne between 375-425). These are structured in three genetic groups (FST=0.158), with high values of the Mc index (0.186-0.283), suggesting ancestral demographic decline events (between 11,910 to 23,820 years ago), associated with the Pleistocene climate changes. Conclusions: The study showed that P. remota contains a considerable genetic variation, which is spatially structured and associated with ancestral bottlenecks. Moreover, it is confirmed that P. catarinae is its synonym. The species has been able to survive and adapt to local environmental conditions. With this knowledge conservation strategies for the remaining populations of the species are proposed. Métodos: El estudio se realizó en siete poblaciones representativas de su distribución natural en México, con muestras de acículas de 112 árboles analizadas con cinco microsatélites de núcleo. Los datos se evaluaron con parámetros de la genética de poblaciones y métodos de aproximación Bayesiana. Resultados clave: La heterocigosidad promedio esperada (He=0.557) fue más alta que la observada (Ho=0.375). Las poblaciones no se encuentran en equilibro Hardy-Weinberg, con una endogamia significativa (FIS=0.259) y tamaños efectivos reducidos (Ne entre 375-425); están estructuradas en tres grupos genéticos (FST=0.158), con valores altos del índice Mc (0.186-0.283), sugiriendo eventos de declinamiento demográfico ancestral (entre 11,910 y 23,820 años atrás), asociados con los cambios climáticos del Pleistoceno. Conclusiones: El estudio demuestra que P. remota contiene una variación genética considerable, pero estructurada espacialmente y asociada a cuellos de botella ancestrales. Además, se confirma que P. catarinae es su sinónimo. La especie ha sido capaz de subsistir y adaptarse a condiciones ambientales locales. Con este conocimiento se plantean estrategias para la conservación de las poblaciones remanentes de la especie. Instituto de Ecología, A.C. 2021-09-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/1890 10.21829/abm128.2021.1890 Acta Botanica Mexicana; No. 128 (2021) Acta Botanica Mexicana; Núm. 128 (2021) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1890/3888 https://abm.ojs.inecol.mx/index.php/abm/article/view/1890/3912 https://abm.ojs.inecol.mx/index.php/abm/article/view/1890/3889 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | González Jiménez, Aridahí Delgado Valerio, Patricia Molina Sánchez, Agustín Flores López, Celestino Vargas Gutiérrez, Juan Pablo García Magaña, J. Jesús Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction |
| title | Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction |
| title_full | Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction |
| title_fullStr | Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction |
| title_full_unstemmed | Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction |
| title_short | Genetic diversity in Pinus remota (Pinaceae) populations of Northeast Mexico: evidences of historical demographic contraction |
| title_sort | genetic diversity in pinus remota (pinaceae) populations of northeast mexico: evidences of historical demographic contraction |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1890 |
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