Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae)
The Corynorhinus mexicanus bat provides an interesting experimental model for the study of epididymal sperm maturation because after testicular regression, this bat stores sperm in epididymal cauda for several months. Earlier research conducted by our group suggested that sperm maturation in this sp...
| Main Authors: | , , , , , |
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| Formato: | Online |
| Idioma: | eng |
| Publicado: |
Instituto de Ecología, A.C.
2020
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| Acceso en liña: | https://azm.ojs.inecol.mx/index.php/azm/article/view/2245 |
| _version_ | 1799770408900100096 |
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| author | Rodríguez-Tobón, Ahiezer Fierro, Reyna León-Galván, Miguel Angel Rosado, Adolfo Cortés-Barberena, Edith Arenas-Ríos, Edith |
| author_facet | Rodríguez-Tobón, Ahiezer Fierro, Reyna León-Galván, Miguel Angel Rosado, Adolfo Cortés-Barberena, Edith Arenas-Ríos, Edith |
| author_sort | Rodríguez-Tobón, Ahiezer |
| collection | AZM |
| description | The Corynorhinus mexicanus bat provides an interesting experimental model for the study of epididymal sperm maturation because after testicular regression, this bat stores sperm in epididymal cauda for several months. Earlier research conducted by our group suggested that sperm maturation in this specie must be completed in the caudal region of the epididymis, although the precise factor linked with this phenomenon is unknown. Therefore, the aim of this work was to analyze changes in the distribution of N-acetylglucosamine and/or sialic acid, Fucose and Mannose carbohydrates in different membrane domains of sperm cells as they change from the caput to the cauda of the epididymis, as well as, their changes in different dates of capture. The sperm cells present a redistribution of N-acetylglucosamine and/or sialic when they arrived in the caudal region (September 11), but after storage until October 22 the distribution of N-acetylglucosamine and/or sialic acid changed. Mannose residues were found predominantly towards the acrosome during their entry into and transit through the three regions of the epididymis. The flow cytometry assay indicated that fluorescence intensity due to the presence of of N-acetylglucosamine and/or sialic acid on the sperm decreases as the sperm pass through the epididymal duct and as storage time in the cauda goes on. The Mannose fluorescence intensity, decreased in corpus and cauda from September 24 to October 8, though no differences appeared on the latter date. The presence of Fucuse was corroborated only by flow cytometry. In conclusion, the carbohydrate distribution on sperm membrane can be considered as part of the process of epididymal sperm maturation and is associated with the phenomenon of prolonged sperm storage that is characteristic of this specie. This adaptation allows the males to synchronize with the period of receptivity of the females, and then, carry out the matings. |
| format | Online |
| id | azm-article-2245 |
| institution | Acta Zoológica Mexicana |
| language | eng |
| publishDate | 2020 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | azm-article-22452022-10-25T19:57:09Z Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) Rodríguez-Tobón, Ahiezer Fierro, Reyna León-Galván, Miguel Angel Rosado, Adolfo Cortés-Barberena, Edith Arenas-Ríos, Edith bats epididymal sperm maturation sperm storage membrane carbohydrates lectins murciélagos maduración espermática epididimaria almacenamiento prolongado carbohidratos de membrana lectinas The Corynorhinus mexicanus bat provides an interesting experimental model for the study of epididymal sperm maturation because after testicular regression, this bat stores sperm in epididymal cauda for several months. Earlier research conducted by our group suggested that sperm maturation in this specie must be completed in the caudal region of the epididymis, although the precise factor linked with this phenomenon is unknown. Therefore, the aim of this work was to analyze changes in the distribution of N-acetylglucosamine and/or sialic acid, Fucose and Mannose carbohydrates in different membrane domains of sperm cells as they change from the caput to the cauda of the epididymis, as well as, their changes in different dates of capture. The sperm cells present a redistribution of N-acetylglucosamine and/or sialic when they arrived in the caudal region (September 11), but after storage until October 22 the distribution of N-acetylglucosamine and/or sialic acid changed. Mannose residues were found predominantly towards the acrosome during their entry into and transit through the three regions of the epididymis. The flow cytometry assay indicated that fluorescence intensity due to the presence of of N-acetylglucosamine and/or sialic acid on the sperm decreases as the sperm pass through the epididymal duct and as storage time in the cauda goes on. The Mannose fluorescence intensity, decreased in corpus and cauda from September 24 to October 8, though no differences appeared on the latter date. The presence of Fucuse was corroborated only by flow cytometry. In conclusion, the carbohydrate distribution on sperm membrane can be considered as part of the process of epididymal sperm maturation and is associated with the phenomenon of prolonged sperm storage that is characteristic of this specie. This adaptation allows the males to synchronize with the period of receptivity of the females, and then, carry out the matings. El murciélago Corynorhinus mexicanus es un modelo interesante para el estudio de la maduración espermática epididimaria, debido a que después de la regresión testicular, este quiróptero almacena los espermatozoides en la cauda epididimaria por varios meses. Nuestro grupo de investigación determino que la maduración epididimaria en este murciélago culmina en la región caudal, aunque el factor preciso que lo determina es aún desconocido. Por lo que, el objetivo de este trabajo fue analizar los cambios en la distribución de carbohidratos de membrana (N-acetilglucosamina y/o ácido siálico, Fucosa y Manosa) en diferentes dominios de la membrana plasmática de los espermatozoides y cómo estos azúcares se distribuyen de caput a cauda del epidídimo, así como los cambios que se presentan durante el periodo de almacenamiento. Los espermatozoides presentan una redistribución de N-acetilglucosamina y/o ácido siálico cuando ingresan a la región caudal del epidídimo (septiembre 11), pero después de almacenadas las células la distribución vuelve a cambiar (octubre 22). Los residuos de Manosa se encontraron predominantemente hacia el acrosoma durante su entrada y tránsito a través de las tres regiones del epidídimo. El análisis por citometría de flujo indicó que, la intensidad de fluorescencia (IF) de N-acetilglucosamina y/o ácido siálico localizada en los espermatozoides disminuye a medida que estas células ingresan al conducto epididimario y transcurre el tiempo de almacenamiento en la cauda epididimaria. En el caso de Manosa, la IF disminuye en el corpus y cauda epididimarios de septiembre 24 a octubre 8, sin diferencias para la última fecha. La presencia de Fucosa sólo se corroboró por citometría de flujo. En conclusión, la distribución de carbohidratos de membrana puede ser considerada como parte del proceso de maduración espermática epididimaria y puede estar asociada al proceso de almacenamiento prolongado, característico de esta especie. Adaptación que le permite a los machos sincronizarse con el periodo de receptividad de las hembras y poder llevar a cabo los apareamientos. Instituto de Ecología, A.C. 2020-02-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original articles Artículos originales application/pdf https://azm.ojs.inecol.mx/index.php/azm/article/view/2245 10.21829/azm.2020.3612245 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 36 (2020); 1-12 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 36 (2020); 1-12 2448-8445 0065-1737 eng https://azm.ojs.inecol.mx/index.php/azm/article/view/2245/2471 Derechos de autor 2020 ACTA ZOOLÓGICA MEXICANA (N.S.) http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Rodríguez-Tobón, Ahiezer Fierro, Reyna León-Galván, Miguel Angel Rosado, Adolfo Cortés-Barberena, Edith Arenas-Ríos, Edith Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) |
| title | Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) |
| title_full | Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) |
| title_fullStr | Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) |
| title_full_unstemmed | Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) |
| title_short | Changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of Corynorhinus mexicanus bat (Chiroptera: Vespertilionidae) |
| title_sort | changes in membrane carbohydrates distribution associated to epididymal sperm maturation during the prolonged sperm storage period of corynorhinus mexicanus bat (chiroptera: vespertilionidae) |
| url | https://azm.ojs.inecol.mx/index.php/azm/article/view/2245 |
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