Transferability of microsatellite markers among nine pine species
There are 94 species of conifers distributed in Mexico, from which Pinus L. species are the most abundant with 49 species. Nevertheless, the genetic diversity studies on Mexican populations of pines are limited, particularly those including molecular markers considered species-specific, such as micr...
| Hlavní autoři: | , , , , |
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| Médium: | Online |
| Jazyk: | spa |
| Vydáno: |
Instituto de Ecología, A.C.
2021
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| On-line přístup: | https://myb.ojs.inecol.mx/index.php/myb/article/view/2103 |
| _version_ | 1799769403188838400 |
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| author | Sandoval-Padilla, Isaac Contreras-Toledo, Aremi Rebeca Guzmán, Luis Felipe Amaro González, Blanca Amalia Cortés-Cruz, Moisés |
| author_facet | Sandoval-Padilla, Isaac Contreras-Toledo, Aremi Rebeca Guzmán, Luis Felipe Amaro González, Blanca Amalia Cortés-Cruz, Moisés |
| author_sort | Sandoval-Padilla, Isaac |
| collection | MYB |
| description | There are 94 species of conifers distributed in Mexico, from which Pinus L. species are the most abundant with 49 species. Nevertheless, the genetic diversity studies on Mexican populations of pines are limited, particularly those including molecular markers considered species-specific, such as microsatellites (SSR). The initial cost for their identification is high when the species’ genome has not been sequenced, as well as the designing of primers that allow the amplification of the SSR regions. However, an alternative is the assessment of SSR in related species. The objective of this study was to evaluate the transferability of SSR markers developed for P. taeda in different pine species. Genomic DNA was isolated from P. ayacahuite, P. cembroides, P. devoniana, P. hartwegii, P. lumholtzii, P. luzmariae, P. patula, P. jeffreyi and P. pseudostrobus, based on CTAB method (ionic detergent cetyltrimethylammonium bromide) from liophylized tissue. The markers were selected based on linkage group (LG), motif and position within the LG. Finally, fragments were amplified by PCR and products were quantified. Thirty-seven markers (95%) amplified in at least one of the nine species. From those, 27 (69%) amplified in more than 50% of the species. These markers were present in the 12 LG. Amplification of more than 75% was observed for P. jeffreyi, P. pseudostrobus and P. devoniana. The transferability of these markers represents an alternative for genetic diversity studies across pine species. |
| format | Online |
| id | oai:oai.myb.ojs.inecol.mx:article-2103 |
| institution | Madera y Bosques |
| language | spa |
| publishDate | 2021 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.myb.ojs.inecol.mx:article-21032022-11-29T22:17:42Z Transferability of microsatellite markers among nine pine species Transferibilidad de marcadores de microsatélites en nueve especies de pinos Sandoval-Padilla, Isaac Contreras-Toledo, Aremi Rebeca Guzmán, Luis Felipe Amaro González, Blanca Amalia Cortés-Cruz, Moisés amplificación inter-específica diversidad genética Pinus SSR inter-specific amplification genetic diversity genetic SSR There are 94 species of conifers distributed in Mexico, from which Pinus L. species are the most abundant with 49 species. Nevertheless, the genetic diversity studies on Mexican populations of pines are limited, particularly those including molecular markers considered species-specific, such as microsatellites (SSR). The initial cost for their identification is high when the species’ genome has not been sequenced, as well as the designing of primers that allow the amplification of the SSR regions. However, an alternative is the assessment of SSR in related species. The objective of this study was to evaluate the transferability of SSR markers developed for P. taeda in different pine species. Genomic DNA was isolated from P. ayacahuite, P. cembroides, P. devoniana, P. hartwegii, P. lumholtzii, P. luzmariae, P. patula, P. jeffreyi and P. pseudostrobus, based on CTAB method (ionic detergent cetyltrimethylammonium bromide) from liophylized tissue. The markers were selected based on linkage group (LG), motif and position within the LG. Finally, fragments were amplified by PCR and products were quantified. Thirty-seven markers (95%) amplified in at least one of the nine species. From those, 27 (69%) amplified in more than 50% of the species. These markers were present in the 12 LG. Amplification of more than 75% was observed for P. jeffreyi, P. pseudostrobus and P. devoniana. The transferability of these markers represents an alternative for genetic diversity studies across pine species. En México se encuentran presentes 94 especies de coníferas, de las cuales el género Pinus L. destaca con 49. No obstante, los estudios de diversidad genética de poblaciones mexicanas de pinos son escasos, particularmente aquellos que incluyen marcadores moleculares, basados en secuencias simples repetidas (SSR), considerados especie-específicos, como los microsatélites. El costo inicial para su identificación es elevado cuando no se ha secuenciado el genoma de las especies, así como el diseño de cebadores que permitan la amplificación de las regiones SSR. Sin embargo, una alternativa es la evaluación de los SSRs en especies relacionadas. El objetivo del presente trabajo fue evaluar la transferibilidad de marcadores SSR desarrollados para P. taeda en diferentes especies de pinos. Se extrajo ADN genómico de P. ayacahuite, P. cembroides, P. devoniana, P. hartwegii, P. lumholtzii, P. luzmariae, P. patula, P. jeffreyi y P. pseudostrobus. basado en el método de CTAB (bromuro de hexadeciltrimetilamonio) a partir de tejido liofilizado Los marcadores fueron seleccionados por grupos de ligamiento (GL), por su motivo de repetición y por su posición dentro de cada GL. Finalmente, los fragmentos amplificados por PCR fueron cuantificados. Treinta y siete marcadores (95%) amplificaron en al menos una de las nueve especies evaluadas. De ellos, 27 (69%) presentaron amplificación en más de 50% de las especies. Estos marcadores presentan cobertura en los 12 GL. Se observó amplificación de más de 75% en P. jeffreyi, P. pseudostrobus y P. devoniana. La transferibilidad de estos marcadores representa una alternativa para realizar estudios de diversidad genética en especies de pinos. Instituto de Ecología, A.C. 2021-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares application/pdf text/xml https://myb.ojs.inecol.mx/index.php/myb/article/view/2103 10.21829/myb.2021.2732103 Madera y Bosques; Vol. 27 No. 3 (2021): Autumn 2021; e2732103 Madera y Bosques; Vol. 27 Núm. 3 (2021): Otoño 2021; e2732103 2448-7597 1405-0471 spa https://myb.ojs.inecol.mx/index.php/myb/article/view/2103/2326 https://myb.ojs.inecol.mx/index.php/myb/article/view/2103/2347 10.21829/myb.2018.243566 10.21829/myb.2019.252618 Derechos de autor 2021 Madera y Bosques http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Sandoval-Padilla, Isaac Contreras-Toledo, Aremi Rebeca Guzmán, Luis Felipe Amaro González, Blanca Amalia Cortés-Cruz, Moisés Transferability of microsatellite markers among nine pine species |
| title | Transferability of microsatellite markers among nine pine species |
| title_full | Transferability of microsatellite markers among nine pine species |
| title_fullStr | Transferability of microsatellite markers among nine pine species |
| title_full_unstemmed | Transferability of microsatellite markers among nine pine species |
| title_short | Transferability of microsatellite markers among nine pine species |
| title_sort | transferability of microsatellite markers among nine pine species |
| url | https://myb.ojs.inecol.mx/index.php/myb/article/view/2103 |
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