Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity
In order to obtain strains of the edible mushroom Pleurotus ostreatus that can be cultivated in pine wood shavings, 36 monosporic cultures were isolated and propagated in vitro, selecting 6 cultures of rapid mycelial growth in order to obtain interspecimen crosses and dikaryon-monokaryon mating. Ele...
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| Formaat: | Online |
| Taal: | eng |
| Gepubliceerd in: |
Instituto de Ecología, A.C.
2019
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| Online toegang: | https://myb.ojs.inecol.mx/index.php/myb/article/view/e2521715 |
| _version_ | 1799769355092754432 |
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| author | Mata, Gerardo Pérez-Torres, José Antonio Medel, Rosario Pérez-Merlo, Rosalía Salmones, Dulce |
| author_facet | Mata, Gerardo Pérez-Torres, José Antonio Medel, Rosario Pérez-Merlo, Rosalía Salmones, Dulce |
| author_sort | Mata, Gerardo |
| collection | MYB |
| description | In order to obtain strains of the edible mushroom Pleurotus ostreatus that can be cultivated in pine wood shavings, 36 monosporic cultures were isolated and propagated in vitro, selecting 6 cultures of rapid mycelial growth in order to obtain interspecimen crosses and dikaryon-monokaryon mating. Eleven of the crosses obtained and their 6 parental strains were cultured in vitro in order to determine their growth in pine shavings and barley straw (control), selecting 6 strains (3 crosses, 3 parental strains) for sporome production in the two growth substrates. At in vitro level, significant differences were found between the mycelial areas of monokaryons and dikaryons in the treatments (time, substrate) evaluated. In terms of sporome productivity, the cultures in barley straw presented higher biological efficiency (22.6%-46.4%) and production rate (0.31%-0.77%) values than those in the pine shavings (3.3%-12% and 0.03%-0.11%, respectively). Sporome pileus diameters of 5 cm to 9.9 cm were the most frequent in both substrates. In general, the crosses surpassed their parents in both mycelial growth and sporome production, although further study is required in order to facilitate increased productivity of the strains in this potentially useful agroforestry residue. |
| format | Online |
| id | oai:oai.myb.ojs.inecol.mx:article-1715 |
| institution | Madera y Bosques |
| language | eng |
| publishDate | 2019 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.myb.ojs.inecol.mx:article-17152022-11-29T22:48:26Z Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity Cultivo de Pleurotus ostreatus en viruta de pino: obtención de cepas y evaluación de su productividad Mata, Gerardo Pérez-Torres, José Antonio Medel, Rosario Pérez-Merlo, Rosalía Salmones, Dulce crosses culture edible fungi Mexico mushrooms pine shavings cruzas cultivo hongos comestibles México setas viruta de pino In order to obtain strains of the edible mushroom Pleurotus ostreatus that can be cultivated in pine wood shavings, 36 monosporic cultures were isolated and propagated in vitro, selecting 6 cultures of rapid mycelial growth in order to obtain interspecimen crosses and dikaryon-monokaryon mating. Eleven of the crosses obtained and their 6 parental strains were cultured in vitro in order to determine their growth in pine shavings and barley straw (control), selecting 6 strains (3 crosses, 3 parental strains) for sporome production in the two growth substrates. At in vitro level, significant differences were found between the mycelial areas of monokaryons and dikaryons in the treatments (time, substrate) evaluated. In terms of sporome productivity, the cultures in barley straw presented higher biological efficiency (22.6%-46.4%) and production rate (0.31%-0.77%) values than those in the pine shavings (3.3%-12% and 0.03%-0.11%, respectively). Sporome pileus diameters of 5 cm to 9.9 cm were the most frequent in both substrates. In general, the crosses surpassed their parents in both mycelial growth and sporome production, although further study is required in order to facilitate increased productivity of the strains in this potentially useful agroforestry residue. Con el fin de obtener cepas del hongo comestible Pleurotus ostreatus con capacidad de cultivarse en viruta de pino, se aislaron y propagaron in vitro 36 cultivos monospóricos, seleccionándose 6 cultivos de rápido crecimiento micelial para obtener cruzas interespecimen y dicarión-monocarión. Once de las cruzas obtenidas más sus 6 parentales fueron cultivadas in vitro para determinar sus áreas miceliales en viruta de pino y paja de cebada (testigo), seleccionándose seis cepas (3 cruzas, 3 parentales) para producir sus esporomas en viruta de pino y paja de cebada (testigo). A nivel in vitro, se encontraron diferencias significativas entre las áreas miceliales de monocariones y dicariones en los tratamientos (tiempo, sustrato) evaluados. En cuanto a productividad de los esporomas, los cultivos en paja de cebada alcanzaron valores más altos de eficiencias biológicas (22.6%-46.4%) y tasas de producción (0.31% - 0.77%) que en que viruta de pino (3.3% - 12%) y (0.03% - 0.11%), respectivamente. Los esporomas con diámetros de píleo de 5 cm a 9.9 cm de largo fueron más abundantes en ambos sustratos. En general, las cruzas superaron a sus parentales tanto en crecimiento micelial como en producción de esporomas, aunque se requiere más estudios que permitan incrementar la productividad de las cepas en este potencial residuo agroforestal. Instituto de Ecología, A.C. 2019-09-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares application/pdf application/xml https://myb.ojs.inecol.mx/index.php/myb/article/view/e2521715 10.21829/myb.2019.2521715 Madera y Bosques; Vol. 25 No. 2 (2019): Verano 2019 Madera y Bosques; Vol. 25 Núm. 2 (2019): Verano 2019 2448-7597 1405-0471 eng https://myb.ojs.inecol.mx/index.php/myb/article/view/e2521715/1964 https://myb.ojs.inecol.mx/index.php/myb/article/view/e2521715/1953 Derechos de autor 2019 Madera y Bosques |
| spellingShingle | Mata, Gerardo Pérez-Torres, José Antonio Medel, Rosario Pérez-Merlo, Rosalía Salmones, Dulce Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| title | Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| title_full | Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| title_fullStr | Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| title_full_unstemmed | Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| title_short | Culture of Pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| title_sort | culture of pleurotus ostreatus in pine shavings: isolation of strains and evaluation of their productivity |
| url | https://myb.ojs.inecol.mx/index.php/myb/article/view/e2521715 |
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