Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote
Total phenolics, flavonoids and proanthocyanidins from aqueous acetone 70% (crude extract) and semi purified extracts by liquid-liquid partition with ethyl acetate (organic fraction) of Pinus cooperi, Pinus engelmannii, Pinus leiophylla and Pinus teocote bark were evaluated. The antioxidant activity...
| Main Authors: | , , , , , |
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| Format: | Online |
| Jezik: | spa |
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Instituto de Ecología, A.C.
2016
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| Online dostop: | https://myb.ojs.inecol.mx/index.php/myb/article/view/1187 |
| _version_ | 1799769223236419584 |
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| author | Rosales-Castro, Martha González-Laredo, Rubén Francisco Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto Peralta-Cruz, Javier Karchesy, Joseph J. |
| author_facet | Rosales-Castro, Martha González-Laredo, Rubén Francisco Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto Peralta-Cruz, Javier Karchesy, Joseph J. |
| author_sort | Rosales-Castro, Martha |
| collection | MYB |
| description | Total phenolics, flavonoids and proanthocyanidins from aqueous acetone 70% (crude extract) and semi purified extracts by liquid-liquid partition with ethyl acetate (organic fraction) of Pinus cooperi, Pinus engelmannii, Pinus leiophylla and Pinus teocote bark were evaluated. The antioxidant activity using acid 2,2´-azinobis-3-etilbenzotiazolin-6sulfonic radical (ABTS•+), deoxyribose assay (scavenging of hydroxyl radical) and human Low Density Lipoprotein (LDL) inhibition were also determined. Chromatographic analysis of High Pressure Liquid Chromatography (HPLC) of extracts was performed. Total phenolics concentration was 491 mg g-1 to 604 mg g-1, organic extracts showed higher flavonoids concentration (292 mg g-1 to 385 mg g-1) than crude ones (259 mg g-1 to 314 mg g-1), while proanthocyanidins concentration was higher in crude extracts (186 mg g-1 to 286 mg g-1) than organic extracts (70 mg g-1 to 151 mg g1). The radical scavenging activity ABTS was (49,48% to 57,44%), similar to catechin standard (57,92%). The scavenging capacity of OH radical (25,85% to 48,46%), was higher in organic extracts for all species. The LDL oxidation inhibition for organic extracts (64,41% to 89,39%), was higher than crude extracts. Catechin was identified in all species at low concentration. HPLC chromatograms showed a flavanone as the major compound in P. cooperi, P. engelmannii, and P. teocote bark extracts. |
| format | Online |
| id | oai:oai.myb.ojs.inecol.mx:article-1187 |
| institution | Madera y Bosques |
| language | spa |
| publishDate | 2016 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.myb.ojs.inecol.mx:article-11872022-11-30T00:23:15Z Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote Evaluación química y capacidad antioxidante de extractos polifenólicos de cortezas Rosales-Castro, Martha González-Laredo, Rubén Francisco Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto Peralta-Cruz, Javier Karchesy, Joseph J. ABTS bark Low Density Lipoprotein (LDL) Pinus polyphenols ABTS corteza Lipoproteínas de Baja Densidad (LDL) Pinus polifenoles Total phenolics, flavonoids and proanthocyanidins from aqueous acetone 70% (crude extract) and semi purified extracts by liquid-liquid partition with ethyl acetate (organic fraction) of Pinus cooperi, Pinus engelmannii, Pinus leiophylla and Pinus teocote bark were evaluated. The antioxidant activity using acid 2,2´-azinobis-3-etilbenzotiazolin-6sulfonic radical (ABTS•+), deoxyribose assay (scavenging of hydroxyl radical) and human Low Density Lipoprotein (LDL) inhibition were also determined. Chromatographic analysis of High Pressure Liquid Chromatography (HPLC) of extracts was performed. Total phenolics concentration was 491 mg g-1 to 604 mg g-1, organic extracts showed higher flavonoids concentration (292 mg g-1 to 385 mg g-1) than crude ones (259 mg g-1 to 314 mg g-1), while proanthocyanidins concentration was higher in crude extracts (186 mg g-1 to 286 mg g-1) than organic extracts (70 mg g-1 to 151 mg g1). The radical scavenging activity ABTS was (49,48% to 57,44%), similar to catechin standard (57,92%). The scavenging capacity of OH radical (25,85% to 48,46%), was higher in organic extracts for all species. The LDL oxidation inhibition for organic extracts (64,41% to 89,39%), was higher than crude extracts. Catechin was identified in all species at low concentration. HPLC chromatograms showed a flavanone as the major compound in P. cooperi, P. engelmannii, and P. teocote bark extracts. Se evaluó la concentración de fenoles totales, flavonoides y proantocianidinas en extractos de acetona acuosa 70% (extracto crudo) y extractos semipurificados por partición líquido-líquido con acetato de etilo (extracto orgánico), de cortezas de Pinus cooperi, Pinus engelmannii, Pinus leiophylla y Pinus teocote, asimismo se determinó la actividad antioxidante de los extractos por las técnicas de radical ácido 2,2´-azinobis-3-etilbenzotiazolin-6-sulfónico (ABTS•+), desoxi-d-ribosa (atrapamiento de radical hidroxilo), y por la inhibición de la oxidación de lipoproteínas de baja densidad (LDL). Se realizó una comparación cromatográfica de los extractos por Cromatografía Líquida de Alta Resolución (HPLC). La concentración de fenoles fue de 491 mg g-1 a 604 mg g-1, los extractos orgánicos presentaron mayor concentración de flavonoides (292 mg g-1 a 385 mg g-1) que los extractos crudos (259 mg g-1 a 314 mg g-1) , mientras que la concentración de proantocianidinas fue mayor en el extracto crudo (186 mg g-1a 286 mg g-1) que en el orgánico (70 mg g-1a 151 mg g-1) . La capacidad de captura del radical ABTS fue de 49,48% a 57,44%, similares al que presentó el estándar catequina (57,92 %). La capacidad de captura del radical hidroxilo varió de 25,85% a 48,46% y fue mayor en el extracto orgánico en todas las especies. La inhibición de oxidación de LDL fue de 64,41% a 89,39%, con valores más altos en el extracto orgánico. Los cromatogramas de HPLC muestran semejanza de los compuestos químicos en las cuatro especies. Se identificó el flavanol catequina a baja concentración en todas las especies. El compuesto principal en P. cooperi, P. engelmannii, y P. teocote, es similar en las tres especies y por espectro de UV corresponde a una flavanona. Instituto de Ecología, A.C. 2016-08-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares application/pdf https://myb.ojs.inecol.mx/index.php/myb/article/view/1187 10.21829/myb.2009.1531187 Madera y Bosques; Vol. 15 No. 3 (2009): Invierno 2009; 87-105 Madera y Bosques; Vol. 15 Núm. 3 (2009): Invierno 2009; 87-105 2448-7597 1405-0471 spa https://myb.ojs.inecol.mx/index.php/myb/article/view/1187/1366 Derechos de autor 2016 Madera y Bosques |
| spellingShingle | Rosales-Castro, Martha González-Laredo, Rubén Francisco Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto Peralta-Cruz, Javier Karchesy, Joseph J. Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote |
| title | Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote |
| title_full | Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote |
| title_fullStr | Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote |
| title_full_unstemmed | Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote |
| title_short | Chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of Pinus cooperi, P. engelmannii, P. leiophylla and P. teocote |
| title_sort | chemical evaluation and antioxidant capacity of polypehenolic extracts from bark of pinus cooperi, p. engelmannii, p. leiophylla and p. teocote |
| url | https://myb.ojs.inecol.mx/index.php/myb/article/view/1187 |
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