Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote
Coniferous trees are a vast resource in Durango, Mexico forests and a potential nontimber source of these chemicals, thus, our study for analyzing phenolic content and biological activity in needle extracts from Pinus cooperi (COO), P. durangensis (DUR), P. engelmanii (ENG) and P. teocote ( TEO). Pi...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Instituto de Ecología, A.C.
2016
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| Online Access: | https://myb.ojs.inecol.mx/index.php/myb/article/view/1165 |
| _version_ | 1799769216828571648 |
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| author | Sáenz-Esqueda, María de los Ángeles Rosales-Castro, Martha Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto González-Laredo, Rubén Francisco |
| author_facet | Sáenz-Esqueda, María de los Ángeles Rosales-Castro, Martha Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto González-Laredo, Rubén Francisco |
| author_sort | Sáenz-Esqueda, María de los Ángeles |
| collection | MYB |
| description | Coniferous trees are a vast resource in Durango, Mexico forests and a potential nontimber source of these chemicals, thus, our study for analyzing phenolic content and biological activity in needle extracts from Pinus cooperi (COO), P. durangensis (DUR), P. engelmanii (ENG) and P. teocote ( TEO). Pine needles were dewaxed with hexane and extracted with aqueous acetone and methanol. DUR and ENG extracts showed the higher yields, while acetone TEO and methanol ENG extracts showed the highest catechin equivalent (CE) contents. A partial chromatographic purification on acetone TEO extracts produced five fractions, the third one (F3-TEO) had the highest phenolic content (489,6 CE). In condensed tannins contents, acetone and methanol TEO extracts, as well fraction F3-TEO were the best (24,3, 15.7 and 44,4 mg/g extract, respectively). In DPPH activity, the more effective CE50 were obtained from TEO acetone extract (223,5 ppm) and fractions F3-TEO (145,0 ppm) and F4-TEO (150,8 ppm). In ABTS assay, TEO acetone (27,7%) and methanolic (18,2%) extracts, and fractions F3-TEO (60,1%) and F4-TEO (55,8%) were the best. In LDL assay the highest inhibitory activity was shown by acetone extracts, particularly ENG (99,4%) at 90 ppm. Therefore acetone extracts from TEO and ENG may have utilization as a potential source of bioactive phenolics. |
| format | Online |
| id | oai:oai.myb.ojs.inecol.mx:article-1165 |
| institution | Madera y Bosques |
| language | spa |
| publishDate | 2016 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.myb.ojs.inecol.mx:article-11652022-11-30T00:19:53Z Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote Contenido fenólico y acción antioxidante de extractos de acículas de Pinus cooperi, P. durangensis, P. engelmanii y P. teocote Sáenz-Esqueda, María de los Ángeles Rosales-Castro, Martha Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto González-Laredo, Rubén Francisco ABTS Antioxidants DPPH LDL needles polyphenols ABTS acículas antioxidante DPPH LDL polifenoles Coniferous trees are a vast resource in Durango, Mexico forests and a potential nontimber source of these chemicals, thus, our study for analyzing phenolic content and biological activity in needle extracts from Pinus cooperi (COO), P. durangensis (DUR), P. engelmanii (ENG) and P. teocote ( TEO). Pine needles were dewaxed with hexane and extracted with aqueous acetone and methanol. DUR and ENG extracts showed the higher yields, while acetone TEO and methanol ENG extracts showed the highest catechin equivalent (CE) contents. A partial chromatographic purification on acetone TEO extracts produced five fractions, the third one (F3-TEO) had the highest phenolic content (489,6 CE). In condensed tannins contents, acetone and methanol TEO extracts, as well fraction F3-TEO were the best (24,3, 15.7 and 44,4 mg/g extract, respectively). In DPPH activity, the more effective CE50 were obtained from TEO acetone extract (223,5 ppm) and fractions F3-TEO (145,0 ppm) and F4-TEO (150,8 ppm). In ABTS assay, TEO acetone (27,7%) and methanolic (18,2%) extracts, and fractions F3-TEO (60,1%) and F4-TEO (55,8%) were the best. In LDL assay the highest inhibitory activity was shown by acetone extracts, particularly ENG (99,4%) at 90 ppm. Therefore acetone extracts from TEO and ENG may have utilization as a potential source of bioactive phenolics. Durango es uno de los estados con mayor riqueza forestal, siendo el pino el género dominante en sus bosques, de ahí el interés por evaluar química y biológicamente extractos de acículas de Pinus cooperi (COO), P. durangensis (DUR), P. engelmanii (ENG) y P. teocote (TEO). Se practicaron extracciones sucesivas con acetona y metanol acuosos de acículas de pino desengrasadas previamente con hexano, en las cuales DUR y ENG mostraron respectivamente los mejores rendimientos. Los valores más altos del contenido de fenoles totales fueron los extraíbles acetónicos de TEO y los metanólicos de ENG. Se realizó una purificación cromatográfica a los extraíbles acetónicos de TEO, generando cinco fracciones; F3-TEO con 489,6 EC presentó el mayor contenido fenólico. Los extractos acetónicos y metanólicos de TEO y la fracción purificada F3-TEO presentaron la mayor concentración de taninos condensados. En el ensayo de DPPH, los extractos más relevantes según su concentrappm) y F4-TEO (150,8 ppm). De manera similarción efectiva media (CE 50) fueron los acetónicos de , en el ensayo de TEO (223,5 ppm) y las fracciones F3-TEO (145,0 ABTS, los extractos de TEO en acetona (27,7%) y en metanol (18,2%), así como las fracciones F3-TEO (60,1%) y F4-TEO (55,8%) mostraron la supresión más elevada de la oxidación. En el ensayo de la oxidación de LDL, la mayor actividad inhibitoria fue mostrada por extractos acetónicos, particularmente por ENG (99,4%) a una concentración de 90 ppm. Los extractos acetónicos de TEO y de ENG presentan potencial como fuente de polifenoles bioactivos. Instituto de Ecología, A.C. 2016-08-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares application/pdf https://myb.ojs.inecol.mx/index.php/myb/article/view/1165 10.21829/myb.2010.1631165 Madera y Bosques; Vol. 16 No. 3 (2010): Otoño 2010; 37-48 Madera y Bosques; Vol. 16 Núm. 3 (2010): Otoño 2010; 37-48 2448-7597 1405-0471 spa https://myb.ojs.inecol.mx/index.php/myb/article/view/1165/1345 Derechos de autor 2016 Madera y Bosques |
| spellingShingle | Sáenz-Esqueda, María de los Ángeles Rosales-Castro, Martha Rocha-Guzmán, Nuria Elizabeth Gallegos-Infante, José Alberto González-Laredo, Rubén Francisco Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote |
| title | Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote |
| title_full | Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote |
| title_fullStr | Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote |
| title_full_unstemmed | Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote |
| title_short | Phenolic content and antioxidant action in needle extracts from Pinus cooperi, P. durangensis, P. engelmanii and P. teocote |
| title_sort | phenolic content and antioxidant action in needle extracts from pinus cooperi, p. durangensis, p. engelmanii and p. teocote |
| url | https://myb.ojs.inecol.mx/index.php/myb/article/view/1165 |
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