Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen
The effect of some sources of organic nitrogen such as hydrolyzed casein and the aminoacids L-glutamine and L-Serine were tested in the process of direct secondary somatic embryogenesis, in which somatic embryos of Nothofagus alpina -induced from isolated cotyledons of mature seeds- were used as exp...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Instituto de Ecología, A.C.
2016
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| Online Access: | https://myb.ojs.inecol.mx/index.php/myb/article/view/1147 |
| _version_ | 1799769210307477504 |
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| author | Castellanos, Hermes Sánchez-Olate, Manuel Wehenkel, Christian Corral, Javier |
| author_facet | Castellanos, Hermes Sánchez-Olate, Manuel Wehenkel, Christian Corral, Javier |
| author_sort | Castellanos, Hermes |
| collection | MYB |
| description | The effect of some sources of organic nitrogen such as hydrolyzed casein and the aminoacids L-glutamine and L-Serine were tested in the process of direct secondary somatic embryogenesis, in which somatic embryos of Nothofagus alpina -induced from isolated cotyledons of mature seeds- were used as explants. The highest percentages of direct secondary embryogenesis were obtained in BTM base medium plus MS vitamins, without plant growth regulators, and in this medium plus NAA, BAP and hydrolyzed casein. However, the fresh weight of the proliferated mass was greatest in the presence of an additional source of organic nitrogen. The highest average number of embryos per explant was also produced in the treatment with growth regulators and hydrolyzed casein, although the number was not significantly higher than in the L-Glutamine treatment, in which significantly less callus growth occurred. Alternate use of base medium and NAA, BAP and hydrolyzed casein medium during subculture enabled direct proliferation of the embryogenic culture, which remained stable for over two years and with a low incidence of callus. |
| format | Online |
| id | oai:oai.myb.ojs.inecol.mx:article-1147 |
| institution | Madera y Bosques |
| language | spa |
| publishDate | 2016 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.myb.ojs.inecol.mx:article-11472022-11-30T00:12:04Z Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen Proliferación de embriones somáticos de Nothofagus alpina: efecto de la fuente de nitrógeno orgánico exógena Castellanos, Hermes Sánchez-Olate, Manuel Wehenkel, Christian Corral, Javier Somatic embryogenesis developmental stage micropropagation growth regulators Embriogénesis somática estado de desarrollo micropropagación reguladores del crecimiento The effect of some sources of organic nitrogen such as hydrolyzed casein and the aminoacids L-glutamine and L-Serine were tested in the process of direct secondary somatic embryogenesis, in which somatic embryos of Nothofagus alpina -induced from isolated cotyledons of mature seeds- were used as explants. The highest percentages of direct secondary embryogenesis were obtained in BTM base medium plus MS vitamins, without plant growth regulators, and in this medium plus NAA, BAP and hydrolyzed casein. However, the fresh weight of the proliferated mass was greatest in the presence of an additional source of organic nitrogen. The highest average number of embryos per explant was also produced in the treatment with growth regulators and hydrolyzed casein, although the number was not significantly higher than in the L-Glutamine treatment, in which significantly less callus growth occurred. Alternate use of base medium and NAA, BAP and hydrolyzed casein medium during subculture enabled direct proliferation of the embryogenic culture, which remained stable for over two years and with a low incidence of callus. Se probó el efecto de algunas fuentes de nitrógeno orgánico como caseína hidrolizada y los aminoácidos L-Glutamina y L-Serina en el proceso de embriogénesis somática secundaria directa, empleando como explantes embriones somáticos de Nothofagus alpina inducidos desde cotiledones aislados de semillas maduras. Los mayores porcentajes de embriogénesis secundaria directa fueron obtenidos en medio base BTM y vitaminas MS, sin reguladores del crecimiento vegetal y en este medio más ANA, BAP y caseína hidrolizada. Sin embargo, el peso fresco de las masas de proliferación fue superior en presencia de una fuente adicional de nitrógeno orgánico. Bajo el tratamiento con reguladores del crecimiento y caseína hidrolizada también se produjo la mayor cantidad promedio de embriones producidos por explanto, aunque no hubo diferencias significativas con el tratamiento que contuvo L-Glutamina, donde el crecimiento de callo fue significativamente menor. Mediante subcultivos alternados entre medio base y medio con ANA, BAP y caseína hidrolizada, se logró la proliferación del cultivo embriogénico de manera directa, estable durante más de dos años y con baja incidencia de callo. Instituto de Ecología, A.C. 2016-08-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares application/pdf https://myb.ojs.inecol.mx/index.php/myb/article/view/1147 10.21829/myb.2011.1721147 Madera y Bosques; Vol. 17 No. 2 (2011): Verano 2011; 35-46 Madera y Bosques; Vol. 17 Núm. 2 (2011): Verano 2011; 35-46 2448-7597 1405-0471 spa https://myb.ojs.inecol.mx/index.php/myb/article/view/1147/1328 Derechos de autor 2016 Madera y Bosques |
| spellingShingle | Castellanos, Hermes Sánchez-Olate, Manuel Wehenkel, Christian Corral, Javier Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen |
| title | Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen |
| title_full | Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen |
| title_fullStr | Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen |
| title_full_unstemmed | Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen |
| title_short | Proliferation of somatic embryos of Nothofagus alpina: effect of an exogen source of organic nitrogen |
| title_sort | proliferation of somatic embryos of nothofagus alpina: effect of an exogen source of organic nitrogen |
| url | https://myb.ojs.inecol.mx/index.php/myb/article/view/1147 |
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