Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski
Pinus maximartinezii Rzedowski is a nut pine native to Mexico that currently is considered as an endangered species because of disturbance of populations and natural habitat, as well as its limited geographic distribution. Even though such technologies as tissue culture could represent an alternativ...
| Päätekijät: | , , |
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| Aineistotyyppi: | Online |
| Kieli: | spa |
| Julkaistu: |
Instituto de Ecología, A.C.
2009
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| Linkit: | https://abm.ojs.inecol.mx/index.php/abm/article/view/305 |
| _version_ | 1799771127317266432 |
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| author | Robledo Paz, Alejandrina Villalobos Arámbula, Víctor Manuel Santacruz Varela, Amalio |
| author_facet | Robledo Paz, Alejandrina Villalobos Arámbula, Víctor Manuel Santacruz Varela, Amalio |
| author_sort | Robledo Paz, Alejandrina |
| collection | ABM |
| description | Pinus maximartinezii Rzedowski is a nut pine native to Mexico that currently is considered as an endangered species because of disturbance of populations and natural habitat, as well as its limited geographic distribution. Even though such technologies as tissue culture could represent an alternative for its conservation and propagation, the existing literature for this pine is scarce. In this paper a protocol that allows an efficient differentiation of adventitious shoots for this species is presented. Different types of explants (embryos and cotyledons) were evaluated regarding their capacity to differentiate adventitious shoots. Explants from cotyledons showed the best response, so they were cultivated in a modified Schenk and Hildebrandt (SH) medium supplemented with four different concentrations (2.2-16.8 mg L-1) of N6-benzyladenine (BA). Percentages of explants forming shoots and number of shoots developed per explant were statistically different among the tested concentrations. The 16.8 mg L-1 of BA concentration induced the largest percentage of explants forming shoots (53.5) and the highest number of shoots developed per explant (29.1) after 14 weeks of culture. Three percent of the individual shoots produced roots. The developed methodology allows differentiation of adventitious shoots of Pinus maximartinezii from cotyledon explants, with a multiplication rate significantly higher than the one obtained by other authors. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-305 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2009 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-3052022-12-23T05:37:41Z Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski Inducción eficiente de brotes adventicios en cotiledones de Pinus maximartinezii Rzedowski Robledo Paz, Alejandrina Villalobos Arámbula, Víctor Manuel Santacruz Varela, Amalio micropropagation organogenesis Pinus maximartinezii tissue culture cultivo de tejidos micropropagación organogénesis Pinus maximartinezii Pinus maximartinezii Rzedowski is a nut pine native to Mexico that currently is considered as an endangered species because of disturbance of populations and natural habitat, as well as its limited geographic distribution. Even though such technologies as tissue culture could represent an alternative for its conservation and propagation, the existing literature for this pine is scarce. In this paper a protocol that allows an efficient differentiation of adventitious shoots for this species is presented. Different types of explants (embryos and cotyledons) were evaluated regarding their capacity to differentiate adventitious shoots. Explants from cotyledons showed the best response, so they were cultivated in a modified Schenk and Hildebrandt (SH) medium supplemented with four different concentrations (2.2-16.8 mg L-1) of N6-benzyladenine (BA). Percentages of explants forming shoots and number of shoots developed per explant were statistically different among the tested concentrations. The 16.8 mg L-1 of BA concentration induced the largest percentage of explants forming shoots (53.5) and the highest number of shoots developed per explant (29.1) after 14 weeks of culture. Three percent of the individual shoots produced roots. The developed methodology allows differentiation of adventitious shoots of Pinus maximartinezii from cotyledon explants, with a multiplication rate significantly higher than the one obtained by other authors. Pinus maximartinezii Rzedowski es una especie de piñonero originaria de México, considerada en peligro de extinción debido a la alteración de sus poblaciones y de su hábitat, así como a su distribución geográfica restringida. A pesar de que tecnologías como el cultivo de tejidos podrían ser una alternativa para su conservación y propagación, la literatura existente al respecto es escasa. En este trabajo se presenta un protocolo que permite la diferenciación eficiente de brotes adventicios de este taxon. Se probaron embriones y cotiledones en cuanto a su capacidad para formar yemas adventicias. Los segundos mostraron la mejor respuesta y fueron entonces cultivados en el medio de Schenk y Hildebrandt (SH) modificado, suplementado con cuatro concentraciones (2.2 a 16.8 mg L-1) deN6-benciladenina (BA). El porcentaje de explantes que produjeron brotes y el número de brotes formados en éstos fue estadísticamente diferente entre los niveles de BA probados; con 16.8 mg L-1 se indujo el mayor porcentaje de órganos que formaron brotes (53.5) y el número más alto de brotes generados por explante (29.1) a las 14 semanas de cultivo; de los vástagos individualizados, 3% formó raíces. La metodología desarrollada permite la diferenciación de yemas adventicias de P. maximartinezii a partir del cultivo de cotiledones, con una tasa de multiplicación significativamente superior a la obtenida por otros autores. Instituto de Ecología, A.C. 2009-10-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/html https://abm.ojs.inecol.mx/index.php/abm/article/view/305 10.21829/abm89.2009.305 Acta Botanica Mexicana; No. 89 (2009); 47-62 Acta Botanica Mexicana; Núm. 89 (2009); 47-62 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/305/472 https://abm.ojs.inecol.mx/index.php/abm/article/view/305/473 Derechos de autor 2015 Acta Botanica Mexicana |
| spellingShingle | Robledo Paz, Alejandrina Villalobos Arámbula, Víctor Manuel Santacruz Varela, Amalio Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski |
| title | Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski |
| title_full | Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski |
| title_fullStr | Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski |
| title_full_unstemmed | Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski |
| title_short | Efficient induction of adventitious shoots in cotyledons of Pinus maximartinezii Rzedowski |
| title_sort | efficient induction of adventitious shoots in cotyledons of pinus maximartinezii rzedowski |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/305 |
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