Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia
In this research an in vitro technique was developed for production and propagation of intergeneric hybrids from immature seeds produced by crossing the domestic sunflower Helianthus annuus and the wild species Tithonia rotundifolia (Mill.) S. F. Blake. For embryo rescue in tissue culture the greate...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Instituto de Ecología, A.C.
2010
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| Online Access: | https://abm.ojs.inecol.mx/index.php/abm/article/view/277 |
| _version_ | 1799771117333774336 |
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| author | Gómez-Martínez, Martha Reyes-Valdés, M. Humberto Martínez-Reyna, Juan Manuel Escobedo-Bocardo, Leticia García-Osuna, Hermila T. |
| author_facet | Gómez-Martínez, Martha Reyes-Valdés, M. Humberto Martínez-Reyna, Juan Manuel Escobedo-Bocardo, Leticia García-Osuna, Hermila T. |
| author_sort | Gómez-Martínez, Martha |
| collection | ABM |
| description | In this research an in vitro technique was developed for production and propagation of intergeneric hybrids from immature seeds produced by crossing the domestic sunflower Helianthus annuus and the wild species Tithonia rotundifolia (Mill.) S. F. Blake. For embryo rescue in tissue culture the greatest success was obtained with the sequential use of three Murashige-Skoog based media: a) with amino acids and growth regulator BAP for germination, b) without amino acids and with BAP for micropropagation, and c) without vitamins and growth regulator ANA for root development. The amino acids were added as a source of organic nitrogen. From a single seedling a total of 130 healthy plantlets were obtained. Twenty of them were taken for acclimatization, and only one reached an incomplete flowering stage. Therefore, standardizing acclimatization conditions is still required. In particular, it is necessary to establish treatments that allow a clear definition of the specific conditions for the acclimatization of the hybrid, through the test of different substrate mixtures and nutritive solutions under controlled environmental conditions. It is recommended to try the use of retardants and amino acids, as well as modifications in light intensity. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-277 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2010 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-2772022-12-23T05:30:57Z Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia Rescate de embriones en híbridos intergenéricos Helianthus annuus x Tithonia rotundifolia Gómez-Martínez, Martha Reyes-Valdés, M. Humberto Martínez-Reyna, Juan Manuel Escobedo-Bocardo, Leticia García-Osuna, Hermila T. embryo rescue Helianthus annuus intergeneric crossing Tithonia rotundifolia cruzamientos intergenéricos Helianthus annuus rescate de embriones Tithonia rotundifolia In this research an in vitro technique was developed for production and propagation of intergeneric hybrids from immature seeds produced by crossing the domestic sunflower Helianthus annuus and the wild species Tithonia rotundifolia (Mill.) S. F. Blake. For embryo rescue in tissue culture the greatest success was obtained with the sequential use of three Murashige-Skoog based media: a) with amino acids and growth regulator BAP for germination, b) without amino acids and with BAP for micropropagation, and c) without vitamins and growth regulator ANA for root development. The amino acids were added as a source of organic nitrogen. From a single seedling a total of 130 healthy plantlets were obtained. Twenty of them were taken for acclimatization, and only one reached an incomplete flowering stage. Therefore, standardizing acclimatization conditions is still required. In particular, it is necessary to establish treatments that allow a clear definition of the specific conditions for the acclimatization of the hybrid, through the test of different substrate mixtures and nutritive solutions under controlled environmental conditions. It is recommended to try the use of retardants and amino acids, as well as modifications in light intensity. En esta investigación se desarrolló una técnica in vitro para producir y propagar híbridos intergenéricos a partir de semillas inmaduras producto del cruzamiento del girasol cultivado Helianthus annuus L. con la especie silvestre Tithonia rotundifolia (Mill.) S. F. Blake. Para el rescate de embriones inmaduros en cultivo de tejidos el mayor éxito se obtuvo con el uso secuencial de tres medios basados en el de Murashige-Skoog: a) con adición de aminoácidos y el regulador de crecimiento BAP para germinación, b) sin aminoácidos y con BAP para micropropagación, y c) sin vitaminas y con el regulador de crecimiento ANA para enraizamiento. Los aminoácidos se agregaron como fuente de nitrógeno orgánico. A partir de una plántula se lograron obtener 130 en buenas condiciones de sanidad. Veinte de ellas se llevaron a aclimatación, y solamente una generó botones y llegó a floración inconclusa, por lo que aún se requiere estandarizar las condiciones de aclimatación. En particular, se necesita establecer tratamientos que permitan definir con claridad las condiciones específicas para aclimatar el híbrido, a través de la prueba de diferentes mezclas de sustratos y soluciones nutritivas bajo condiciones ambientales controladas. Se recomienda probar el manejo de retardantes y aminoácidos, así como realizar modificaciones en la intensidad de luz. Instituto de Ecología, A.C. 2010-10-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/html https://abm.ojs.inecol.mx/index.php/abm/article/view/277 10.21829/abm93.2010.277 Acta Botanica Mexicana; No. 93 (2010); 111-119 Acta Botanica Mexicana; Núm. 93 (2010); 111-119 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/277/416 https://abm.ojs.inecol.mx/index.php/abm/article/view/277/417 Derechos de autor 2015 Acta Botanica Mexicana |
| spellingShingle | Gómez-Martínez, Martha Reyes-Valdés, M. Humberto Martínez-Reyna, Juan Manuel Escobedo-Bocardo, Leticia García-Osuna, Hermila T. Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia |
| title | Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia |
| title_full | Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia |
| title_fullStr | Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia |
| title_full_unstemmed | Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia |
| title_short | Embryo rescue of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia |
| title_sort | embryo rescue of intergeneric hybrids helianthus annuus x tithonia rotundifolia |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/277 |
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