Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae)
Background and Aims: The Clusiaceae family is known for its bioactive compounds with beneficial antioxidant and anti-inflammatory properties. The objectives of this study were to 1) identify and quantify the individual polyphenol compounds in the methanolic extract from peel, pulp and aril of the fr...
| Main Authors: | , , , , , |
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| Format: | Online |
| Jezik: | eng spa |
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Instituto de Ecología, A.C.
2023
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| Online dostop: | https://abm.ojs.inecol.mx/index.php/abm/article/view/2180 |
| _version_ | 1799771430474219520 |
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| author | Martínez-Mendoza, Beatriz Itzel Juárez-Trujillo, Naida Mendoza-López, María Remedios Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio Jiménez Fernández, Maribel |
| author_facet | Martínez-Mendoza, Beatriz Itzel Juárez-Trujillo, Naida Mendoza-López, María Remedios Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio Jiménez Fernández, Maribel |
| author_sort | Martínez-Mendoza, Beatriz Itzel |
| collection | ABM |
| description | Background and Aims: The Clusiaceae family is known for its bioactive compounds with beneficial antioxidant and anti-inflammatory properties. The objectives of this study were to 1) identify and quantify the individual polyphenol compounds in the methanolic extract from peel, pulp and aril of the fruit of Clusia quadrangula by UPLC-MSMS, evaluate their antioxidant properties, 2) analyze the fatty acid profile, minerals composition and 3) determine the antibacterial activity against pathogenic bacteria of the methanolic extract.Methods: Physicochemical properties, antioxidants and bioactive compounds, fatty acid profile and mineral content were analyzed using a Microplate Spectrophotometer, Ultra High Performance Liquid Chromatography coupled with a triple quadrupole Mass Spectrometer, Gas Chromatography coupled with a Mass Spectrometer and Microwave Plasma Atomic Emission Spectroscopy.Key results: Twenty phenolic compounds were identified and quantified plus the precursor shikimic acid (103.55 µg/g solids). Procyanidin B2 (41.56 µg/g solids), (−)-epicatechin (34.07 µg/g solids), and ellagic acid (27.58 µg/g solids) were found in greater quantity in the methanolic extract of the pulp. Palmitic and linoleic acid were the most abundant fatty acids in the pulp, peel, and aril, and linolenic acid was present in the pulp. The pulp also exhibited the highest amount of total polyphenolic compounds (24.33 mg GAE/g) and reducing power evaluated by FRAP (18.697 mg ET/g). Analysis of the minerals revealed that all fractions are rich in magnesium, potassium, sodium, and calcium. The results showed that the methanolic extract of the different parts of the fruit had antibacterial properties against Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. Conclusions: These results indicate that different parts of the C. quadrangula fruit are a rich source of natural antioxidants and possess antibacterial properties. Therefore, it can be considered for use as ingredient or additive in the cosmetic, pharmaceutical, or food industries. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-2180 |
| institution | Acta Botanica Mexicana |
| language | eng spa |
| publishDate | 2023 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-21802024-02-16T07:07:54Z Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) Compuestos bioactivos, propiedades antioxidantes y antibacterianas de pulpa, piel y arilo de la fruta de Clusia quadrangula (Clusiaceae) Martínez-Mendoza, Beatriz Itzel Juárez-Trujillo, Naida Mendoza-López, María Remedios Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio Jiménez Fernández, Maribel ácidos grasos minerales polifenoles propiedades antimicrobianas antimicrobial properties fatty acids minerals polyphenols Background and Aims: The Clusiaceae family is known for its bioactive compounds with beneficial antioxidant and anti-inflammatory properties. The objectives of this study were to 1) identify and quantify the individual polyphenol compounds in the methanolic extract from peel, pulp and aril of the fruit of Clusia quadrangula by UPLC-MSMS, evaluate their antioxidant properties, 2) analyze the fatty acid profile, minerals composition and 3) determine the antibacterial activity against pathogenic bacteria of the methanolic extract.Methods: Physicochemical properties, antioxidants and bioactive compounds, fatty acid profile and mineral content were analyzed using a Microplate Spectrophotometer, Ultra High Performance Liquid Chromatography coupled with a triple quadrupole Mass Spectrometer, Gas Chromatography coupled with a Mass Spectrometer and Microwave Plasma Atomic Emission Spectroscopy.Key results: Twenty phenolic compounds were identified and quantified plus the precursor shikimic acid (103.55 µg/g solids). Procyanidin B2 (41.56 µg/g solids), (−)-epicatechin (34.07 µg/g solids), and ellagic acid (27.58 µg/g solids) were found in greater quantity in the methanolic extract of the pulp. Palmitic and linoleic acid were the most abundant fatty acids in the pulp, peel, and aril, and linolenic acid was present in the pulp. The pulp also exhibited the highest amount of total polyphenolic compounds (24.33 mg GAE/g) and reducing power evaluated by FRAP (18.697 mg ET/g). Analysis of the minerals revealed that all fractions are rich in magnesium, potassium, sodium, and calcium. The results showed that the methanolic extract of the different parts of the fruit had antibacterial properties against Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. Conclusions: These results indicate that different parts of the C. quadrangula fruit are a rich source of natural antioxidants and possess antibacterial properties. Therefore, it can be considered for use as ingredient or additive in the cosmetic, pharmaceutical, or food industries. Antecedentes y Objetivos: La familia Clusiaceae es conocida por sus compuestos bioactivos con propiedades antioxidantes y antiinflamatorias beneficiosas. Los objetivos de este estudio fueron: 1) identificar y cuantificar compuestos polifenólicos individuales en el extracto metanólico de cáscara, pulpa y arilo del fruto de Clusia quadrangula por UPLC-MSMS, evaluar sus propiedades antioxidantes, 2) analizar el perfil de ácidos grasos, la composición mineral y 3) determinar la actividad antibacteriana contra bacterias patógenas del extracto metanólico. Métodos: Las propiedades fisicoquímicas, antioxidantes y compuestos bioactivos, perfil de ácidos grasos y contenido de minerales fueron analizados utilizando un Espectrofotómetro de Microplaca, Cromatografía de Líquidos de Ultra Alta Resolución acoplado a un Espectrómetro de Masas triple cuadrupolo, Cromatografía de Gases acoplada a un Espectrómetro de Masas y Espectroscopia de Emisión Atómica de Plasma de Microondas.Resultados clave: Se identificaron y cuantificaron 20 compuestos fenólicos más el precursor ácido shikímico (103.55 µg/g sólidos). Procianidina B2 (41.56 µg/g sólidos), (−)-epicatequina (34.07 µg/g sólidos) y ácido elágico (27.58 µg/g sólidos) se encontraron en mayor cantidad en el extracto metanólico de la pulpa. Los ácidos palmítico y linoleico fueron los ácidos grasos más abundantes en la pulpa, cáscara y arilo, y el ácido linolénico estuvo presente en la pulpa. La pulpa también exhibió la mayor cantidad de compuestos polifenólicos totales (24.33 mg GAE/g) y poder reductor evaluado por FRAP (18.697 mg ET/g). El análisis de los minerales reveló que todas las fracciones son ricas en magnesio, potasio, sodio y calcio. Los resultados mostraron que el extracto metanólico de las diferentes partes del fruto tenían propiedades antibacterianas contra Salmonella typhi, Escherichia coli, Staphylococcus aureus, y Enterococcus faecalis.Conclusión: Estos resultados indican que las diferentes partes del fruto de C. quadrangula son una rica fuente de antioxidantes naturales y poseen propiedades antibacterianas, por lo que se puede considerar para su uso como ingrediente o aditivo en la industria cosmética, farmacéutica o alimentaria. Instituto de Ecología, A.C. 2023-07-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/2180 10.21829/abm130.2023.2180 Acta Botanica Mexicana; No. 130 (2023) Acta Botanica Mexicana; Núm. 130 (2023) 2448-7589 0187-7151 eng spa https://abm.ojs.inecol.mx/index.php/abm/article/view/2180/4430 https://abm.ojs.inecol.mx/index.php/abm/article/view/2180/4492 https://abm.ojs.inecol.mx/index.php/abm/article/view/2180/4431 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Martínez-Mendoza, Beatriz Itzel Juárez-Trujillo, Naida Mendoza-López, María Remedios Monribot-Villanueva, Juan Luis Guerrero-Analco, José Antonio Jiménez Fernández, Maribel Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) |
| title | Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) |
| title_full | Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) |
| title_fullStr | Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) |
| title_full_unstemmed | Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) |
| title_short | Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae) |
| title_sort | bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of clusia quadrangula (clusiaceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/2180 |
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