Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia
Background and Aims: The genus Morchella groups ascomycete fungi, with several edible species of great nutritional and biotechnological importance. In China and the United States of America, species of Morchella have been artificially cultivated succesfully until producing fruiting bodies. The objec...
| Main Authors: | , , , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Instituto de Ecología, A.C.
2023
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| Online Access: | https://abm.ojs.inecol.mx/index.php/abm/article/view/2173 |
| _version_ | 1799771429798936576 |
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| author | Ortega-Vargas, Claudia Ivette Santos-Hernández, Antonio Fidel Carrasco-Hernández, Violeta Rodríguez-Trejo, Dante Arturo Máxima Borja de la Rosa, Ma. Amparo García Díaz, Silvia Edith |
| author_facet | Ortega-Vargas, Claudia Ivette Santos-Hernández, Antonio Fidel Carrasco-Hernández, Violeta Rodríguez-Trejo, Dante Arturo Máxima Borja de la Rosa, Ma. Amparo García Díaz, Silvia Edith |
| author_sort | Ortega-Vargas, Claudia Ivette |
| collection | ABM |
| description | Background and Aims: The genus Morchella groups ascomycete fungi, with several edible species of great nutritional and biotechnological importance. In China and the United States of America, species of Morchella have been artificially cultivated succesfully until producing fruiting bodies. The objectives of this work were: identify species of Morchella collected in an Abies religiosa forest, cultivate their mycelium in vitro and propagate them in wheat grains. The present study contributes to the knowledge of the initial phase necessary for the possible artificial cultivation of this group of fungi.
Methods: Mushrooms were collected in an Abies religiosa forest located in Santo Tomas Apipilhuasco, Tepetlaoxtoc, Mexico State, Mexico. Fungi were identified (taxonomically and molecularly) and isolation was realized from pieces from sporome tissue in potato dextrose agar (PDA) medium, followed by propagation in wheat grains.
Key results: The species collected were identified as Morchella tridentina and cultivation in vitro of mycelium in PDA was achieved as well as its propagation in wheat grains, where the formation of sclerotia was observed after 21 days of inoculation. The characteristics of the in vitro-cultured mycelium varied among strains, although they were isolated from specimens corresponding to the same species. The specimens were deposited in the CHAP Herbarium, Universidad Autónoma de Chapingo.
Conclusions: Because of the presence of strains that are capable to form sclerotia, it could be used for ascocarp production. However, it is convenient to continue with this investigation with the objective of determining the optimal conditions (environmental, substrates, fruiting induction) for this aim and to achieve its artificial cultivation. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-2173 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2023 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-21732024-02-16T07:59:14Z Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia Registro de Morchella tridentina en un bosque de Abies religiosa en México y cultivo in vitro de su micelio con obtención de esclerocios Ortega-Vargas, Claudia Ivette Santos-Hernández, Antonio Fidel Carrasco-Hernández, Violeta Rodríguez-Trejo, Dante Arturo Máxima Borja de la Rosa, Ma. Amparo García Díaz, Silvia Edith biotecnología colmenilla hongos comestibles. biotechnology colmenilla edible mushrooms. Background and Aims: The genus Morchella groups ascomycete fungi, with several edible species of great nutritional and biotechnological importance. In China and the United States of America, species of Morchella have been artificially cultivated succesfully until producing fruiting bodies. The objectives of this work were: identify species of Morchella collected in an Abies religiosa forest, cultivate their mycelium in vitro and propagate them in wheat grains. The present study contributes to the knowledge of the initial phase necessary for the possible artificial cultivation of this group of fungi. Methods: Mushrooms were collected in an Abies religiosa forest located in Santo Tomas Apipilhuasco, Tepetlaoxtoc, Mexico State, Mexico. Fungi were identified (taxonomically and molecularly) and isolation was realized from pieces from sporome tissue in potato dextrose agar (PDA) medium, followed by propagation in wheat grains. Key results: The species collected were identified as Morchella tridentina and cultivation in vitro of mycelium in PDA was achieved as well as its propagation in wheat grains, where the formation of sclerotia was observed after 21 days of inoculation. The characteristics of the in vitro-cultured mycelium varied among strains, although they were isolated from specimens corresponding to the same species. The specimens were deposited in the CHAP Herbarium, Universidad Autónoma de Chapingo. Conclusions: Because of the presence of strains that are capable to form sclerotia, it could be used for ascocarp production. However, it is convenient to continue with this investigation with the objective of determining the optimal conditions (environmental, substrates, fruiting induction) for this aim and to achieve its artificial cultivation. Antecedentes y Objetivos: El género Morchella agrupa hongos ascomicetos, con varias especies comestibles de gran importancia alimenticia y biotecnológica. En China y Estados Unidos de América se han logrado cultivar exitosamente especies de Morchella de forma artificial hasta producir cuerpos fructíferos. Los objetivos del presente trabajo fueron identificar las especies de Morchella recolectadas en bosque de Abies religiosa, cultivar su micelio in vitro y propagarlo en granos de trigo. El presente estudio contribuye al conocimiento de la fase inicial necesaria para el posible cultivo artificial de este grupo de hongos. Métodos: Se recolectaron hongos en un bosque de Abies religiosa ubicado en Santo Tomás Apipilhuasco, Tepetlaoxtoc, Estado de México, México. Los hongos se identificaron (taxonómica- y molecularmente) y se realizaron aislamientos a partir de trozos de tejido del ascocarpo en medio papa dextrosa agar (PDA) y posteriormente se realizó su propagación en granos de trigo. Resultados clave: Las especies recolectadas fueron identificadas como Morchella tridentina y se logró el cultivo de micelio in vitro en medio PDA, así como su propagación en granos de trigo, en donde se observó la formación de esclerocios después de 21 días de inoculación. Las características del micelio que se cultivó in vitro variaron entre las cepas, no obstante que se aislaron de especímenes pertenecientes a una misma especie. Los especímenes deshidratados fueron depositados en el Herbario CHAP que pertenece a la Universidad Autónoma de Chapingo. Conclusiones: Debido a que las presentes cepas fueron capaces de formar esclerocios, podrían ser utilizadas para la producción de ascocarpos. Sin embargo, es conveniente continuar con la investigación, con el objetivo de determinar las condiciones óptimas (ambientales, sustratos y de inducción de la fructificación) para ese fin y lograr su cultivo artificial. Instituto de Ecología, A.C. 2023-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/2173 10.21829/abm130.2023.2173 Acta Botanica Mexicana; No. 130 (2023) Acta Botanica Mexicana; Núm. 130 (2023) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/2173/4474 https://abm.ojs.inecol.mx/index.php/abm/article/view/2173/4498 https://abm.ojs.inecol.mx/index.php/abm/article/view/2173/4475 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Ortega-Vargas, Claudia Ivette Santos-Hernández, Antonio Fidel Carrasco-Hernández, Violeta Rodríguez-Trejo, Dante Arturo Máxima Borja de la Rosa, Ma. Amparo García Díaz, Silvia Edith Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia |
| title | Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia |
| title_full | Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia |
| title_fullStr | Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia |
| title_full_unstemmed | Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia |
| title_short | Record of Morchella tridentina in an Abies religiosa forest in Mexico and in vitro culture of its mycelium with obtaining sclerotia |
| title_sort | record of morchella tridentina in an abies religiosa forest in mexico and in vitro culture of its mycelium with obtaining sclerotia |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/2173 |
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