Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae)
Background and Aims: Parkinsonia praecox,“palo brea”, is a medicinal plant distributed in the Mexican Sonoran Desert. However, there is little information about the chemical composition and biological potential of this plant. As a consequence, the objective of the present study was to determine the...
| Main Authors: | , , , , , , , , , |
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| Format: | Online |
| Language: | eng spa |
| Published: |
Instituto de Ecología, A.C.
2022
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| Online Access: | https://abm.ojs.inecol.mx/index.php/abm/article/view/2089 |
| _version_ | 1799771420356509696 |
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| author | López-Romero, Julio Cesár Torres-Moreno, Heriberto Valencia-Rivera, Dora Edith Leyva-Peralta, Mario Alberto Lugo-Sepúlveda, Ramón Efraín Robles-Zepeda, Ramón Enrique Rodríguez-Martínez, Karen Lillian Villegas-Ochoa, Mónica Alejandra Salazar-López, Norma Julieta González-Aguilar, Gustavo Adolfo |
| author_facet | López-Romero, Julio Cesár Torres-Moreno, Heriberto Valencia-Rivera, Dora Edith Leyva-Peralta, Mario Alberto Lugo-Sepúlveda, Ramón Efraín Robles-Zepeda, Ramón Enrique Rodríguez-Martínez, Karen Lillian Villegas-Ochoa, Mónica Alejandra Salazar-López, Norma Julieta González-Aguilar, Gustavo Adolfo |
| author_sort | López-Romero, Julio Cesár |
| collection | ABM |
| description | Background and Aims: Parkinsonia praecox,“palo brea”, is a medicinal plant distributed in the Mexican Sonoran Desert. However, there is little information about the chemical composition and biological potential of this plant. As a consequence, the objective of the present study was to determine the chemical composition, as well as the antioxidant and antiproliferative activity, of P. praecox. Methods: Methanolic extracts of stems (PPS), berries (PPB) and flowers (PPF) were performed. Chemical composition was determined by phytochemical screening, Folin Ciocalteu and UPLC-DAD methods. Antioxidant activity was determined by DPPH, ABTS, ORAC and FRAP methods. Antiproliferative activity was evaluated by MTT against A549 (non-small-cell lung cancer cells), MDA-MB-231 (triple negative breast cancer), PC-3 (adenocarcinoma prostate cancer grade IV), HeLa (human cervical cancer) and L929 (non-cancerous subcutaneous connective tissue) cell lines. Results: Phytochemical screening showed the presence of terpenes, phenolic compounds, flavonoids, tannins and sugars in the extracts. PPS showed the highest (p<0.05) concentration of phenolic compounds (65.5 mg GAE/g), identifying and quantifying quercetin (218.86 µg/g). Additionally, PPS exhibited the highest capacity (p<0.05) to stabilize the DPPH (IC50: 137 µg/ml), ABTS (39.56 µM TE/g), hydroxyl radicals (ORAC: 1777.78 µM TE/g), and to reduce metals (FRAP: 935.6 µM Fe(II)/g). Similar behavior was observed in antiproliferative activity, since PPS presented the highest cytotoxicity (p<0.05): A549 (IC50: 341.3 µg/ml), MDA-MB-231 (IC50: 147.3 µg/ml), PC-3 (IC50: 78.8 µg/ml), HeLa (IC50: 121.6 µg/ml) and L929 (IC50: 93.29 µg/ml). Conclusion: This is the first research where the bioactive compound profile and the biological potential of P. praecox are reported. The results show the strong association between the antioxidant and the antiproliferative activities with the presence of phenolic compounds. This represents a potential support for the development of pharmacological therapies. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-2089 |
| institution | Acta Botanica Mexicana |
| language | eng spa |
| publishDate | 2022 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-20892023-03-17T06:02:18Z Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) Nueva información del perfil de compuestos bioactivos, potencial antioxidante y antiproliferativo de Parkinsonia praecox (Fabaceae) López-Romero, Julio Cesár Torres-Moreno, Heriberto Valencia-Rivera, Dora Edith Leyva-Peralta, Mario Alberto Lugo-Sepúlveda, Ramón Efraín Robles-Zepeda, Ramón Enrique Rodríguez-Martínez, Karen Lillian Villegas-Ochoa, Mónica Alejandra Salazar-López, Norma Julieta González-Aguilar, Gustavo Adolfo biological activities bioactive compounds Sonoran desert medicinal plants actividades biológicas compuestos bioactivos Desierto de Sonora plantas medicinales Background and Aims: Parkinsonia praecox,“palo brea”, is a medicinal plant distributed in the Mexican Sonoran Desert. However, there is little information about the chemical composition and biological potential of this plant. As a consequence, the objective of the present study was to determine the chemical composition, as well as the antioxidant and antiproliferative activity, of P. praecox. Methods: Methanolic extracts of stems (PPS), berries (PPB) and flowers (PPF) were performed. Chemical composition was determined by phytochemical screening, Folin Ciocalteu and UPLC-DAD methods. Antioxidant activity was determined by DPPH, ABTS, ORAC and FRAP methods. Antiproliferative activity was evaluated by MTT against A549 (non-small-cell lung cancer cells), MDA-MB-231 (triple negative breast cancer), PC-3 (adenocarcinoma prostate cancer grade IV), HeLa (human cervical cancer) and L929 (non-cancerous subcutaneous connective tissue) cell lines. Results: Phytochemical screening showed the presence of terpenes, phenolic compounds, flavonoids, tannins and sugars in the extracts. PPS showed the highest (p<0.05) concentration of phenolic compounds (65.5 mg GAE/g), identifying and quantifying quercetin (218.86 µg/g). Additionally, PPS exhibited the highest capacity (p<0.05) to stabilize the DPPH (IC50: 137 µg/ml), ABTS (39.56 µM TE/g), hydroxyl radicals (ORAC: 1777.78 µM TE/g), and to reduce metals (FRAP: 935.6 µM Fe(II)/g). Similar behavior was observed in antiproliferative activity, since PPS presented the highest cytotoxicity (p<0.05): A549 (IC50: 341.3 µg/ml), MDA-MB-231 (IC50: 147.3 µg/ml), PC-3 (IC50: 78.8 µg/ml), HeLa (IC50: 121.6 µg/ml) and L929 (IC50: 93.29 µg/ml). Conclusion: This is the first research where the bioactive compound profile and the biological potential of P. praecox are reported. The results show the strong association between the antioxidant and the antiproliferative activities with the presence of phenolic compounds. This represents a potential support for the development of pharmacological therapies. Antecedentes y Objetivos: Parkinsonia praecox,“palo brea”, es una planta medicinal distribuida en el Desierto de Sonora en México. Sin embargo, existe poca información acerca de la composición química y del potencial biológico de esta planta. Basado en lo anterior, el objetivo del presente estudio fue determinar la composición química y el potencial antioxidante y antiproliferativo de P. praecox. Métodos: Se prepararon extractos metanólicos de tallos (PPS), frutos (PPB) y flores (PPF). La composición química se determinó a través de los métodos de perfil fitoquímico, Folin-Ciocalteu y UPLC-DAD. La actividad antioxidante se evaluó por los métodos DPPH, ABTS, ORAC y FRAP. La actividad antiproliferativa se determinó por el ensayo MTT contra las líneas celulares A549 (cáncer de pulmón de células no pequeñas), MDA-MB-231 (cáncer de mama triple negativo), PC-3 (cáncer de próstata grado IV), HeLa (cáncer de cervix) y L929 (tejido conectivo subcutáneo no canceroso). Resultados: El perfil fitoquímico mostró la presencia de terpenos, compuestos fenólicos, flavonoides, taninos y azúcares en los extractos. PPS presentó la concentración más elevada (p<0.05) de compuestos fenólicos (65.5 mg GAE/g), identificando y cuantificando quercetina (218.86 µg/g). Además, PPS mostró la capacidad más elevada (p<0.05) para estabilizar a los radicales DPPH (IC50: 137 µg/ml), ABTS (39.56 µM TE/g), hidroxilo (ORAC: 1777.78 µM TE/g), y reducir metales (FRAP: 935.6 µM Fe(II)/g). Un comportamiento similar se observó en la actividad antiproliferativa, ya que PPS mostró la citotoxicidad más elevada (p<0.05): A549 (IC50: 341.3 µg/ml), MDA-MB-231 (IC50: 147.3 µg/ml), PC-3 (IC50: 78.8 µg/ml), HeLa (IC50: 121.6 µg/ml) y L929 (IC50: 93.29 µg/ml).Conclusión: Este es el primer estudio donde se reporta el perfil de compuestos bioactivos y el potencial biológico de P. praecox. Los resultados muestran una fuerte asociación entre la actividad antioxidante y antiproliferativa con la presencia de compuestos fenólicos. Esto representa un potencial soporte para el desarrollo de terapias farmacológicas. Instituto de Ecología, A.C. 2022-12-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/2089 10.21829/abm129.2022.2089 Acta Botanica Mexicana; No. 129 (2022) Acta Botanica Mexicana; Núm. 129 (2022) 2448-7589 0187-7151 eng spa https://abm.ojs.inecol.mx/index.php/abm/article/view/2089/4332 https://abm.ojs.inecol.mx/index.php/abm/article/view/2089/4377 https://abm.ojs.inecol.mx/index.php/abm/article/view/2089/4333 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | López-Romero, Julio Cesár Torres-Moreno, Heriberto Valencia-Rivera, Dora Edith Leyva-Peralta, Mario Alberto Lugo-Sepúlveda, Ramón Efraín Robles-Zepeda, Ramón Enrique Rodríguez-Martínez, Karen Lillian Villegas-Ochoa, Mónica Alejandra Salazar-López, Norma Julieta González-Aguilar, Gustavo Adolfo Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) |
| title | Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) |
| title_full | Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) |
| title_fullStr | Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) |
| title_full_unstemmed | Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) |
| title_short | Novel information about the bioactive compound profile, antioxidant and antiproliferative potential of Parkinsonia praecox (Fabaceae) |
| title_sort | novel information about the bioactive compound profile, antioxidant and antiproliferative potential of parkinsonia praecox (fabaceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/2089 |
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