In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation
Background and Aims: Orchids in Mexico are mainly threatened by deforestation, changes in land use, illegal trade, deficiencies in environmental policy and legislation, and a lack of community participation in the conservation of their forests. Erycina hyalinobulbon is an endemic twig epiphyte orchi...
| Váldodahkkit: | , , |
|---|---|
| Materiálatiipa: | Online |
| Giella: | spa |
| Almmustuhtton: |
Instituto de Ecología, A.C.
2021
|
| Liŋkkat: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1808 |
| _version_ | 1799771390467899392 |
|---|---|
| author | Gutiérrez-Zavala, José Tonatiuh Ávila-Díaz, Irene Magaña-Lemus, Rosa Elia |
| author_facet | Gutiérrez-Zavala, José Tonatiuh Ávila-Díaz, Irene Magaña-Lemus, Rosa Elia |
| author_sort | Gutiérrez-Zavala, José Tonatiuh |
| collection | ABM |
| description | Background and Aims: Orchids in Mexico are mainly threatened by deforestation, changes in land use, illegal trade, deficiencies in environmental policy and legislation, and a lack of community participation in the conservation of their forests. Erycina hyalinobulbon is an endemic twig epiphyte orchid with a short life cycle and with large flowers in relation to its size, for which it has been harvested from its wild populations. The objectives of this work were to evaluate the in vitro development of E. hyalinobulbon in culture media with organic supplements, to compare sucrose vs. N’Joy Stevia® as a carbon source for its initial stages of development, and to evaluate the development of its seedlings in media enriched with plant growth regulators (PGR).Methods: For the sowing of seeds, PhytamaxTM and MS medium at 30% of its basal salts were used in combination with organic supplement (coconut milk, pineapple puree and banana puree), along with the Phy medium used as control. In order to measure the effect of sucrose vs. N´Joy Stevia® as a carbon source, these two treatments were used, with the PhytamaxTM medium. To evaluate the development of seedlings with PGR, three treatments were tested: the 100% PhytamaxTM control, 30% PhytamaxTM with 1.166 ml/l of Maxi-grow and the medium Chiu.Key results: PhytamaxTM medium added with banana promoted germination by 9.3%, being a low cost and easy production option. Ninety days after sowing, N´Joy Stevia® as a carbon source promoted germination by 8%. The best development of the seedlings was registered in the medium PhytamaxTM without PGR.Conclusions: With this study, it was possible to develop an accessible in vitro propagation system for E. hyalinobulbon, in order to sustainably manage it and favor its conservation. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1808 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2021 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-18082022-11-17T00:47:35Z In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation Desarrollo in vitro de la orquídea epífita Erycina hyalinobulbon (Orchidaceae), endémica de México, para promover su conservación Gutiérrez-Zavala, José Tonatiuh Ávila-Díaz, Irene Magaña-Lemus, Rosa Elia Culture media, endemic orchid, in vitro culture, organic supplements, twig epiphyte orchid. Complementos orgánicos, cultivo in vitro, medios de cultivo, orquídea endémica, orquídea epífita de ramilla. Background and Aims: Orchids in Mexico are mainly threatened by deforestation, changes in land use, illegal trade, deficiencies in environmental policy and legislation, and a lack of community participation in the conservation of their forests. Erycina hyalinobulbon is an endemic twig epiphyte orchid with a short life cycle and with large flowers in relation to its size, for which it has been harvested from its wild populations. The objectives of this work were to evaluate the in vitro development of E. hyalinobulbon in culture media with organic supplements, to compare sucrose vs. N’Joy Stevia® as a carbon source for its initial stages of development, and to evaluate the development of its seedlings in media enriched with plant growth regulators (PGR).Methods: For the sowing of seeds, PhytamaxTM and MS medium at 30% of its basal salts were used in combination with organic supplement (coconut milk, pineapple puree and banana puree), along with the Phy medium used as control. In order to measure the effect of sucrose vs. N´Joy Stevia® as a carbon source, these two treatments were used, with the PhytamaxTM medium. To evaluate the development of seedlings with PGR, three treatments were tested: the 100% PhytamaxTM control, 30% PhytamaxTM with 1.166 ml/l of Maxi-grow and the medium Chiu.Key results: PhytamaxTM medium added with banana promoted germination by 9.3%, being a low cost and easy production option. Ninety days after sowing, N´Joy Stevia® as a carbon source promoted germination by 8%. The best development of the seedlings was registered in the medium PhytamaxTM without PGR.Conclusions: With this study, it was possible to develop an accessible in vitro propagation system for E. hyalinobulbon, in order to sustainably manage it and favor its conservation. Antecedentes y Objetivos: Las orquídeas en México están amenazadas principalmente por deforestación, cambios de uso de suelo, comercio ilegal, carencias en la legislación y política ambiental y por una falta de participación de las comunidades para la conservación de sus bosques. Erycina hyalinobulbon es una orquídea endémica epífita de ramilla con un ciclo de vida corto y grandes flores en relación a su tamaño, por lo que ha sido extraída de sus poblaciones silvestres. Los objetivos de este trabajo fueron evaluar el desarrollo in vitro de E. hyalinobulbon en medios de cultivo con suplementos orgánicos, comparar la sacarosa vs. N´Joy Stevia® como fuente de carbono en sus primeras etapas de desarrollo y evaluar el desarrollo de sus plántulas con medios enriquecidos con reguladores de crecimiento vegetal (PGR).Métodos: Para la siembra de semillas los medios de cultivo PhytamaxTM y MS, a 30% de sus sales basales, fueron utilizados con suplementos orgánicos (leche de coco, puré de piña y puré de plátano), utilizando el medio Phy como control. Para poder medir el efecto de la sacarosa vs. N´Joy Stevia®, se utilizaron estos dos tratamientos con el medio PhytamaxTM. Para evaluar el desarrollo de plántulas con PGR, se hicieron tres tratamientos: control de PhytamaxTM 100%, PhytamaxTM 30% con 1.166 ml/l de Maxi-grow y el medio Chiu.Resultados clave: El medio PhytamaxTM adicionado con plátano promovió la germinación en 9.3%, siendo una opción de bajo costo y fácil producción. A los noventa días de efectuada la siembra, N´Joy Stevia® como fuente de carbono promovió la germinación con 8%. El mejor desarrollo de las plántulas se obtuvo en el medio PhytamaxTM sin PGR.Conclusiones: Con este estudio se logró desarrollar un sistema de propagación in vitro de E. hyalinobulbon accesible para poder manejarla sustentablemente y favorecer su conservación. Instituto de Ecología, A.C. 2021-02-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/1808 10.21829/abm128.2021.1808 Acta Botanica Mexicana; No. 128 (2021) Acta Botanica Mexicana; Núm. 128 (2021) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1808/3735 https://abm.ojs.inecol.mx/index.php/abm/article/view/1808/3784 https://abm.ojs.inecol.mx/index.php/abm/article/view/1808/3736 https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Gutiérrez-Zavala, José Tonatiuh Ávila-Díaz, Irene Magaña-Lemus, Rosa Elia In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation |
| title | In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation |
| title_full | In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation |
| title_fullStr | In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation |
| title_full_unstemmed | In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation |
| title_short | In vitro development of the Mexican endemic twig epiphyte Erycina hyalinobulbon (Orchidaceae) to promote its conservation |
| title_sort | in vitro development of the mexican endemic twig epiphyte erycina hyalinobulbon (orchidaceae) to promote its conservation |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1808 |
| work_keys_str_mv | AT gutierrezzavalajosetonatiuh invitrodevelopmentofthemexicanendemictwigepiphyteerycinahyalinobulbonorchidaceaetopromoteitsconservation AT aviladiazirene invitrodevelopmentofthemexicanendemictwigepiphyteerycinahyalinobulbonorchidaceaetopromoteitsconservation AT maganalemusrosaelia invitrodevelopmentofthemexicanendemictwigepiphyteerycinahyalinobulbonorchidaceaetopromoteitsconservation AT gutierrezzavalajosetonatiuh desarrolloinvitrodelaorquideaepifitaerycinahyalinobulbonorchidaceaeendemicademexicoparapromoversuconservacion AT aviladiazirene desarrolloinvitrodelaorquideaepifitaerycinahyalinobulbonorchidaceaeendemicademexicoparapromoversuconservacion AT maganalemusrosaelia desarrolloinvitrodelaorquideaepifitaerycinahyalinobulbonorchidaceaeendemicademexicoparapromoversuconservacion |
