Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae)
Background and Aims: Rhynchostele rossii is an orchid native to Mexico known as gallinitas (little chickens) that is threatened due to overexploitation for ornamental purposes, as a consequence of which it is necessary to realize efforts for its conservation. To date there are no reports of phytoche...
| Những tác giả chính: | , , , , |
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| Định dạng: | Online |
| Ngôn ngữ: | eng spa |
| Được phát hành: |
Instituto de Ecología, A.C.
2020
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| Truy cập trực tuyến: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1665 |
| _version_ | 1799771374142619648 |
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| author | Martínez Cruz, Nieves del Socorro Monribot Villanueva, Juan Luis Cocotle Ronzón, Yolanda Gutiérrez Sánchez, Rosa Angélica Guerrero Analco, José Antonio |
| author_facet | Martínez Cruz, Nieves del Socorro Monribot Villanueva, Juan Luis Cocotle Ronzón, Yolanda Gutiérrez Sánchez, Rosa Angélica Guerrero Analco, José Antonio |
| author_sort | Martínez Cruz, Nieves del Socorro |
| collection | ABM |
| description | Background and Aims: Rhynchostele rossii is an orchid native to Mexico known as gallinitas (little chickens) that is threatened due to overexploitation for ornamental purposes, as a consequence of which it is necessary to realize efforts for its conservation. To date there are no reports of phytochemical studies of this orchid, although it is well known that species of the Orchidaceae family are a good source of bioactive and nutraceutical compounds (e. g. vanillin). Therefore, the main objective of this research was to establish a R. rossii in vitro germination protocol for propagation and determination of phenolic compounds that contribute to its phytochemical knowledge.Methods: A specimen of a wild plant and some seedlings obtained by in vitro culture were dried, milled and their components extracted with MeOH; anti-free radical activity (DPPH), total phenols and flavonoids were determined by spectrophotometric methods, and individual phenolic compounds were identified and quantified by liquid chromatography coupled to mass spectrometry (LC-MS).Key results: The root of the wild plant showed the highest content of total phenols and flavonoids with 121.60 mg GAE g-1 and 108.73 mg CE g-1, respectively, and the best anti-free radical activity with an IC50 53.63 μg ml-1. Extracts from seedlings obtained by in vitro culture also produced phenolic compounds, showing a total phenolic and flavonoid content of 37.35 mg GAE g-1 and 0.16 mg CE g-1, respectively. One coumarin (scopoletin) , three cinnamic acids (4-coumaric acid, ferulic acid and trans-cinnamic acid), three benzoic acids (vanillic acid, vanillin and 4-hydroxybenzoic acid) and three flavonoids (quercetin-3-D-galactoside, quercetin-3-glucoside and kaempferide) were identified and quantified by LC-MS.Conclusions: This study demonstrates that R. rossii is a potential source of antioxidant metabolites that can be obtained by in vitro culture, without harming the wild specimens. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1665 |
| institution | Acta Botanica Mexicana |
| language | eng spa |
| publishDate | 2020 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-16652022-11-25T20:12:09Z Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) Perfil de compuestos fenólicos y actividad antioxidante de Rhynchostele rossii (Orchidaceae) silvestre y cultivada in vitro Martínez Cruz, Nieves del Socorro Monribot Villanueva, Juan Luis Cocotle Ronzón, Yolanda Gutiérrez Sánchez, Rosa Angélica Guerrero Analco, José Antonio biotechnology orchid in vitro propagation secondary metabolites biotecnología metabolitos secundarios propagación in vitro de orquídeas Background and Aims: Rhynchostele rossii is an orchid native to Mexico known as gallinitas (little chickens) that is threatened due to overexploitation for ornamental purposes, as a consequence of which it is necessary to realize efforts for its conservation. To date there are no reports of phytochemical studies of this orchid, although it is well known that species of the Orchidaceae family are a good source of bioactive and nutraceutical compounds (e. g. vanillin). Therefore, the main objective of this research was to establish a R. rossii in vitro germination protocol for propagation and determination of phenolic compounds that contribute to its phytochemical knowledge.Methods: A specimen of a wild plant and some seedlings obtained by in vitro culture were dried, milled and their components extracted with MeOH; anti-free radical activity (DPPH), total phenols and flavonoids were determined by spectrophotometric methods, and individual phenolic compounds were identified and quantified by liquid chromatography coupled to mass spectrometry (LC-MS).Key results: The root of the wild plant showed the highest content of total phenols and flavonoids with 121.60 mg GAE g-1 and 108.73 mg CE g-1, respectively, and the best anti-free radical activity with an IC50 53.63 μg ml-1. Extracts from seedlings obtained by in vitro culture also produced phenolic compounds, showing a total phenolic and flavonoid content of 37.35 mg GAE g-1 and 0.16 mg CE g-1, respectively. One coumarin (scopoletin) , three cinnamic acids (4-coumaric acid, ferulic acid and trans-cinnamic acid), three benzoic acids (vanillic acid, vanillin and 4-hydroxybenzoic acid) and three flavonoids (quercetin-3-D-galactoside, quercetin-3-glucoside and kaempferide) were identified and quantified by LC-MS.Conclusions: This study demonstrates that R. rossii is a potential source of antioxidant metabolites that can be obtained by in vitro culture, without harming the wild specimens. Antecedentes y Objetivos: Rhynchostele rossii es una orquídea nativa de México conocida como gallinitas, que está amenazada debido a su sobreexplotación con fines ornamentales, lo que hace necesario realizar esfuerzos para su conservación. A la fecha, no hay estudios fitoquímicos de esta orquídea, aunque se sabe que las especies de la familia Orchidaceae son una buena fuente de compuestos bioactivos y nutracéuticos (p. ej. vainillina). Por lo anterior, el objetivo principal de esta investigación fue establecer el protocolo de geminación in vitro de R. rossii para la propagación de la especie y la determinación de compuestos fenólicos que contribuyan al conocimiento fitoquímico de esta planta.Métodos: Un ejemplar silvestre y algunas plántulas obtenidas por cultivo in vitro se secaron, molieron y extrajeron con MeOH; se determinó la actividad de anti-radicales libres (DPPH), fenoles y flavonoides totales por métodos espectrofotométricos y algunos fenoles se identificaron y cuantificaron por cromatografía de líquidos acoplada a espectrometría de masas (LC-MS).Resultados clave: La raíz de la planta silvestre mostró el mayor contenido de fenoles y flavonoides totales con 121.60 mg GAE g-1, y 108.73 mg CE g-1, respectivamente, y la mejor actividad anti-radicales libres con una IC50 de 53.63 μg ml-1. Los extractos de las plántulas obtenidas in vitro también produjeron compuestos fenólicos, mostrando un contenido de fenoles y flavonoides totales de 37.35 mg GAE g-1 y 0.16 mg CE g-1, respectivamente, mientras que por LC-MS se identificaron y cuantificaron una cumarina (escopoletina), tres ácidos cinámicos (ácido 4-cumárico, ácido ferúlico y ácido trans-cinamico), tres ácidos benzoicos (ácido vainillico, vainillina y acido 4-hidroxibenzoico) y tres flavonoides (quercetina-3-D-galactósido, quercetina-3-glucósido y kaempferide).Conclusiones: Este estudio muestra que R. rossii es una fuente potencial de metabolitos antioxidantes que pueden obtenerse mediante cultivo in vitro, sin perjudicar a los ejemplares silvestres. Instituto de Ecología, A.C. 2020-05-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf application/epub+zip text/xml https://abm.ojs.inecol.mx/index.php/abm/article/view/1665 10.21829/abm127.2020.1665 Acta Botanica Mexicana; No. 127 (2020) Acta Botanica Mexicana; Núm. 127 (2020) 2448-7589 0187-7151 eng spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1665/pdf https://abm.ojs.inecol.mx/index.php/abm/article/view/1665/3611 https://abm.ojs.inecol.mx/index.php/abm/article/view/1665/3656 Derechos de autor 2020 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Martínez Cruz, Nieves del Socorro Monribot Villanueva, Juan Luis Cocotle Ronzón, Yolanda Gutiérrez Sánchez, Rosa Angélica Guerrero Analco, José Antonio Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) |
| title | Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) |
| title_full | Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) |
| title_fullStr | Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) |
| title_full_unstemmed | Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) |
| title_short | Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae) |
| title_sort | phenolic profile and antioxidant activity from wild and in vitro cultivated rhynchostele rossii (orchidaceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1665 |
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