Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae)
Background and Aims: Flaveria is an American genus with 23 species; in Argentina it is represented by two of them both native and annual: F. bidentis (which is cited as a weed of agroecosystems) and F. haumanii. The genus is mentioned with a base chromosome number of x=18 and cytogenetic backgrounds...
| Autors principals: | , , , |
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| Format: | Online |
| Idioma: | spa |
| Publicat: |
Instituto de Ecología, A.C.
2020
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| Accés en línia: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1567 |
| _version_ | 1799771364928782336 |
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| author | Paez, Valeria de los Angeles Andrada, Aldo R. Sobrero, María T. Chaila, Salvador |
| author_facet | Paez, Valeria de los Angeles Andrada, Aldo R. Sobrero, María T. Chaila, Salvador |
| author_sort | Paez, Valeria de los Angeles |
| collection | ABM |
| description | Background and Aims: Flaveria is an American genus with 23 species; in Argentina it is represented by two of them both native and annual: F. bidentis (which is cited as a weed of agroecosystems) and F. haumanii. The genus is mentioned with a base chromosome number of x=18 and cytogenetic backgrounds for F. bidentis are know, but not so for F. haumanii. The aim of this work was to cytogenetically characterize F. bidentis and F. haumanii through the analysis of mitosis, meiotic behavior and estimation of pollen grain viability.
Methods: The material was collected in the province of Santiago del Estero (Argentina). The root tips were pretreated with 8-hydroxyquinoline 0.002 M for eight hours. Inflorescences and root tips were fixed in Farmer and preserved in cold 70% ethyl alcohol. To stain chromosomes 2% propionic hematoxylin was used. The viability of pollen grains was estimated with the Mützing and DAF stain solutions.
Key results: Flaveria bidentis and F. haumanii have a sporophytic chromosome number of 2n=36, with a symmetric unimodal karyotype. The meiotic behavior was regular, in diakinesis n=18 bivalent was observed and the estimated viability of the pollen grains was greater than 90%.
Conclusions: The sporophytic and gametophytic chromosomal numbers of F. haumanii are mentioned for the first time; in addition, through meiotic analysis the cytogenetic information is completed for F. bidentis. These are the first data related to pollen grain viability. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1567 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2020 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-15672022-11-17T21:46:52Z Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) Caracterización citológica en Flaveria bidentis y F. haumanii (Asteraceae) Paez, Valeria de los Angeles Andrada, Aldo R. Sobrero, María T. Chaila, Salvador cariotipo comportamiento meiótico mitosis viabilidad del polen. karyotype meiotic behavior mitosis pollen viability. Background and Aims: Flaveria is an American genus with 23 species; in Argentina it is represented by two of them both native and annual: F. bidentis (which is cited as a weed of agroecosystems) and F. haumanii. The genus is mentioned with a base chromosome number of x=18 and cytogenetic backgrounds for F. bidentis are know, but not so for F. haumanii. The aim of this work was to cytogenetically characterize F. bidentis and F. haumanii through the analysis of mitosis, meiotic behavior and estimation of pollen grain viability. Methods: The material was collected in the province of Santiago del Estero (Argentina). The root tips were pretreated with 8-hydroxyquinoline 0.002 M for eight hours. Inflorescences and root tips were fixed in Farmer and preserved in cold 70% ethyl alcohol. To stain chromosomes 2% propionic hematoxylin was used. The viability of pollen grains was estimated with the Mützing and DAF stain solutions. Key results: Flaveria bidentis and F. haumanii have a sporophytic chromosome number of 2n=36, with a symmetric unimodal karyotype. The meiotic behavior was regular, in diakinesis n=18 bivalent was observed and the estimated viability of the pollen grains was greater than 90%. Conclusions: The sporophytic and gametophytic chromosomal numbers of F. haumanii are mentioned for the first time; in addition, through meiotic analysis the cytogenetic information is completed for F. bidentis. These are the first data related to pollen grain viability. Antecedentes y Objetivos: Flaveria es un género americano integrado por 23 especies y representado en Argentina por dos de ellas, ambas nativas y anuales: F. bidentis (la cual es considerada maleza de agroecosistemas) y F. haumanii. El género tiene un número cromosómico básico de x=18 y los antecedentes citológicos son referidos a F. bidentis, pero no así para F. haumanii. El objetivo de este trabajo fue caracterizar citogenéticamente a F. bidentis y F. haumanii a través del análisis cariotípico, el comportamiento meiótico y la estimación de la viabilidad de los granos de polen.Métodos: El material fue recolectado en la provincia de Santiago del Estero (Argentina). Las raíces fueron pretratadas con 8-hidroxiquinoleina 0.002 M durante ocho horas. Inflorescencias y raíces fueron fijadas en Farmer y conservadas en alcohol etílico al 70% en frío. Para la tinción de los cromosomas se utilizó hematoxilina propiónica al 2%. La viabilidad de los granos de polen se estimó mediante las soluciones colorantes de Mützing y DAF.Resultados clave: Flaveria bidentis y F. haumanii poseen como número cromosómico esporofítico 2n=36, con un cariotipo unimodal simétrico. El comportamiento meiótico es regular y en diacinesis se observó n=18 bivalentes; la viabilidad estimada de los granos de polen fue superior a 90%.Conclusiones: Se mencionan por primera vez los números cromosómicos esporofíticos y gametofíticos de F. haumanii; además, se completa la informacióncitogenética para F. bidentis a través del análisis de la meiosis. Estos son los primeros datos relacionados con la viabilidad de los granos de polen. Instituto de Ecología, A.C. 2020-01-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf application/xml application/epub+zip https://abm.ojs.inecol.mx/index.php/abm/article/view/1567 10.21829/abm127.2020.1567 Acta Botanica Mexicana; No. 127 (2020) Acta Botanica Mexicana; Núm. 127 (2020) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1567/pdf https://abm.ojs.inecol.mx/index.php/abm/article/view/1567/1964 https://abm.ojs.inecol.mx/index.php/abm/article/view/1567/epub Derechos de autor 2020 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Paez, Valeria de los Angeles Andrada, Aldo R. Sobrero, María T. Chaila, Salvador Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) |
| title | Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) |
| title_full | Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) |
| title_fullStr | Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) |
| title_full_unstemmed | Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) |
| title_short | Cytological characterization in Flaveria bidentis and F. haumanii (Asteraceae) |
| title_sort | cytological characterization in flaveria bidentis and f. haumanii (asteraceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1567 |
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