Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae)
Background and Aims: Castilleja tenuiflora is one of the Mexican, wild and medicinal plants cultivated in vitro which have anti-inflammatory and antiulcerogenic biological activity. The encapsulation of microstakes is an option to propagate and prolong the storage of this plant. Therefore, the objec...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Instituto de Ecología, A.C.
2019
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| Online Access: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1442 |
| _version_ | 1799771351993548800 |
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| author | León Romero, Yatzil Trejo Espino, José Luis Salcedo-Morales, Guadalupe Trejo-Tapia, Gabriela Evangelista Lozano, Silvia |
| author_facet | León Romero, Yatzil Trejo Espino, José Luis Salcedo-Morales, Guadalupe Trejo-Tapia, Gabriela Evangelista Lozano, Silvia |
| author_sort | León Romero, Yatzil |
| collection | ABM |
| description | Background and Aims: Castilleja tenuiflora is one of the Mexican, wild and medicinal plants cultivated in vitro which have anti-inflammatory and antiulcerogenic biological activity. The encapsulation of microstakes is an option to propagate and prolong the storage of this plant. Therefore, the objective of this study was to establish a method for the encapsulation and conservation of C. tenuiflora microstakes that does not affect the content of the compounds of interest of this plant.Methods: Capsules were elaborated with sodium alginate in complex with calcium chloride dihydrate as matrix and tissue microstakes from seedlings grown in vitro. Different concentrations and polymerization times were tested, as well as different storage times and temperature, for later analysis of the major metabolites of the methanolic extracts by chromatography (HPLC) of the resulting plants.Key results: The combination for the production of capsules with microstakes is 3% sodium alginate and calcium chloride dihydrate at 50 mM, with 25 minutes of polymerization. The seedlings derived from synthetic seeds and stored for 100 days presented the same compounds as the donor plants.Conclusions: The encapsulation of C. tenuiflora microstakes with sodium alginate and calcium chloride dihydrate allows the storage of plant material and the main compounds are maintained. Hence, this method for the encapsulation and conservation of microstakes is an alternative to maintain the genotype of elite plants and counteract the illegal collection of wild plants. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1442 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2019 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-14422022-11-17T22:31:06Z Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae) León Romero, Yatzil Trejo Espino, José Luis Salcedo-Morales, Guadalupe Trejo-Tapia, Gabriela Evangelista Lozano, Silvia conversion of seedlings encapsulation micropropagation seedling growth conversión de plántulas crecimiento de plántulas encapsulado micropropagación Background and Aims: Castilleja tenuiflora is one of the Mexican, wild and medicinal plants cultivated in vitro which have anti-inflammatory and antiulcerogenic biological activity. The encapsulation of microstakes is an option to propagate and prolong the storage of this plant. Therefore, the objective of this study was to establish a method for the encapsulation and conservation of C. tenuiflora microstakes that does not affect the content of the compounds of interest of this plant.Methods: Capsules were elaborated with sodium alginate in complex with calcium chloride dihydrate as matrix and tissue microstakes from seedlings grown in vitro. Different concentrations and polymerization times were tested, as well as different storage times and temperature, for later analysis of the major metabolites of the methanolic extracts by chromatography (HPLC) of the resulting plants.Key results: The combination for the production of capsules with microstakes is 3% sodium alginate and calcium chloride dihydrate at 50 mM, with 25 minutes of polymerization. The seedlings derived from synthetic seeds and stored for 100 days presented the same compounds as the donor plants.Conclusions: The encapsulation of C. tenuiflora microstakes with sodium alginate and calcium chloride dihydrate allows the storage of plant material and the main compounds are maintained. Hence, this method for the encapsulation and conservation of microstakes is an alternative to maintain the genotype of elite plants and counteract the illegal collection of wild plants. Antecedentes y Objetivos: Castilleja tenuiflora es una de las plantas mexicanas, silvestres y medicinales cultivadas in vitro que presentan actividad biológica antiinflamatoria y antiulcerogénica. La encapsulación de microestacas es una opción para propagar y prolongar el almacenamiento de esta planta. El objetivo de este estudio fue establecer un método para la encapsulación y conservación de microestacas de C. tenuiflora sin afectar el contenido de sus compuestos.Métodos: Las cápsulas fueron elaboradas con alginato de sodio en complejo con cloruro de calcio dihidratado como matriz y microestacas de tejido proveniente de plántulas cultivadas in vitro. Se probaron diferentes concentraciones y tiempos de polimerización, así como diferentes tiempos de almacenamiento y temperatura, para posteriormente analizar los metabolitos mayoritarios de los extractos metanólicos mediante cromatografía (HPLC) de las plantas resultantes.Resultados clave: La combinación para la producción de cápsulas con microestacas es alginato de sodio al 3% y cloruro de calcio dihidratado al 50 mM, con 25 minutos de polimerización. Las plántulas derivadas de semillas sintéticas, almacenadas por 100 días presentaron los mismos compuestos que las plantas donantes.Conclusiones: La encapsulación de microestacas de C. tenuiflora con alginato de sodio y cloruro de calcio dihidratado permite el almacenamiento del material vegetal y se mantienen sus compuestos principales, por lo que es una alternativa para mantener el genotipo de plantas elite y contrarrestar el saqueo de plantas silvestres. Instituto de Ecología, A.C. 2019-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf application/epub+zip application/xml https://abm.ojs.inecol.mx/index.php/abm/article/view/1442 10.21829/abm126.2019.1442 Acta Botanica Mexicana; No. 126 (2019) Acta Botanica Mexicana; Núm. 126 (2019) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1442/4041 https://abm.ojs.inecol.mx/index.php/abm/article/view/1442/4042 https://abm.ojs.inecol.mx/index.php/abm/article/view/1442/1787 Derechos de autor 2019 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | León Romero, Yatzil Trejo Espino, José Luis Salcedo-Morales, Guadalupe Trejo-Tapia, Gabriela Evangelista Lozano, Silvia Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) |
| title | Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) |
| title_full | Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) |
| title_fullStr | Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) |
| title_full_unstemmed | Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) |
| title_short | Optimization of the production of capsules with microstakes to increase the storage time of the Mexican medicinal plant Castilleja tenuiflora (Orobanchaceae) |
| title_sort | optimization of the production of capsules with microstakes to increase the storage time of the mexican medicinal plant castilleja tenuiflora (orobanchaceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1442 |
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