Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico
Background and Aims: The genus Bursera (~100 species) has its center of diversification and endemism in Mexico. Interspecific hybridization is a frequent phenomenon in Bursera in areas where related species coexist. Hybridization on the one hand can reinforce reproductive barriers, increase genetic...
| Główni autorzy: | , |
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| Format: | Online |
| Język: | eng |
| Wydane: |
Instituto de Ecología, A.C.
2018
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| Dostęp online: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1435 |
| _version_ | 1799771350978527232 |
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| author | Rico, Yessica Reyes Estanislao, Leticia |
| author_facet | Rico, Yessica Reyes Estanislao, Leticia |
| author_sort | Rico, Yessica |
| collection | ABM |
| description | Background and Aims: The genus Bursera (~100 species) has its center of diversification and endemism in Mexico. Interspecific hybridization is a frequent phenomenon in Bursera in areas where related species coexist. Hybridization on the one hand can reinforce reproductive barriers, increase genetic variation, generate novel ecotypes and new lineages, and on the other hand, can lead to maladaptation. However, the ecological and evolutionary consequences of natural hybridization critically depend on hybrid fitness. In this study, within a putative hybrid population in the tropical dry forest of the Bajío region in Mexico and as a proxy of hybrid fitness, we investigated pollen viability among Bursera cuneata, B. bipinnata, and their putative hybrid.Methods: We used two techniques: a pollen staining test with tetrazolium chloride 1% and an in vitro germination test to observe the formation of pollen tubes. Viability percentages were calculated for each group; statistical tests were conducted with general linear models. Key results: Results demonstrated that the putative hybrid is not sterile and exhibited higher germination rates relative to the parental species. Conclusions: Our results imply that gene flow between the parental species and the putative hybrid is likely to occur. Future genetic studies should confirm the genetic identity and ploidy levels of hybrids and evaluate whether genetic introgression has occurred. Our study demonstrates that Bursera hybrids can be fertile and contributes towards understanding the role of hybridization and reproductive isolation in Mexican Bursera species. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1435 |
| institution | Acta Botanica Mexicana |
| language | eng |
| publishDate | 2018 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-14352022-11-25T21:27:37Z Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico Viabilidad y germinación de polen en probables híbridos de Bursera (sección Bullockia; Burseraceae) en México Rico, Yessica Reyes Estanislao, Leticia copales hybrid fertility hybrid zone in vitro germination staining method tropical dry forest bosque tropical caducifolio copales fertilidad en híbridos germinación in vitro técnica de tinción zonas de hibridación Background and Aims: The genus Bursera (~100 species) has its center of diversification and endemism in Mexico. Interspecific hybridization is a frequent phenomenon in Bursera in areas where related species coexist. Hybridization on the one hand can reinforce reproductive barriers, increase genetic variation, generate novel ecotypes and new lineages, and on the other hand, can lead to maladaptation. However, the ecological and evolutionary consequences of natural hybridization critically depend on hybrid fitness. In this study, within a putative hybrid population in the tropical dry forest of the Bajío region in Mexico and as a proxy of hybrid fitness, we investigated pollen viability among Bursera cuneata, B. bipinnata, and their putative hybrid.Methods: We used two techniques: a pollen staining test with tetrazolium chloride 1% and an in vitro germination test to observe the formation of pollen tubes. Viability percentages were calculated for each group; statistical tests were conducted with general linear models. Key results: Results demonstrated that the putative hybrid is not sterile and exhibited higher germination rates relative to the parental species. Conclusions: Our results imply that gene flow between the parental species and the putative hybrid is likely to occur. Future genetic studies should confirm the genetic identity and ploidy levels of hybrids and evaluate whether genetic introgression has occurred. Our study demonstrates that Bursera hybrids can be fertile and contributes towards understanding the role of hybridization and reproductive isolation in Mexican Bursera species. Antecedentes y Objetivos: El género Bursera (~100 especies) tiene su centro de diversificación y endemismo en México. La hibridación interespecífica es un fenómeno común en Bursera en áreas donde coexisten especies relacionadas. La hibridación, por un lado, puede reforzar las barreras reproductivas, aumentar la variación genética, generar ecotipos novedosos y nuevos linajes y, por otro lado, puede conducir a una mala adaptación. Sin embargo, las consecuencias ecológicas y evolutivas de la hibridación natural dependen críticamente de la aptitud de los híbridos. En este estudio, dentro de una población de posibles híbridos en el bosque tropical seco de la región del Bajío en México y utilizando una aproximación de aptitud híbrida, investigamos la viabilidad del polen entre Bursera cuneata, B. bipinnata, y su respectivo híbrido hipotético.Métodos: Utilizamos dos técnicas: una prueba de tinción de polen con cloruro de tetrazolio 1% y una prueba de germinación in vitro para observar la formación de tubos de polen. Se calcularon porcentajes de viabilidad para cada grupo; las pruebas estadísticas se realizaron con modelos lineales generales.Resultados clave: Los resultados demuestran que el híbrido hipotético no es estéril y que presenta mayores tasas de germinación en comparación con las especies parentales.Conclusiones: Nuestros resultados sugieren que el flujo genético entre las especies parentales y el híbrido hipotético es probable. Futuros estudios genéticos deben confirmar el origen genético y nivel de poliploidía de los híbridos y determinar si existe introgresión genética. Nuestro estudio demuestra que los híbridos de Bursera pueden ser fértiles, por lo que contribuye a comprender el papel de la hibridación y el aislamiento reproductivo en las especies mexicanas de Bursera. Instituto de Ecología, A.C. 2018-12-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf application/epub+zip application/xml https://abm.ojs.inecol.mx/index.php/abm/article/view/1435 10.21829/abm126.2019.1435 Acta Botanica Mexicana; No. 126 (2019) Acta Botanica Mexicana; Núm. 126 (2019) 2448-7589 0187-7151 eng https://abm.ojs.inecol.mx/index.php/abm/article/view/1435/4020 https://abm.ojs.inecol.mx/index.php/abm/article/view/1435/4021 https://abm.ojs.inecol.mx/index.php/abm/article/view/1435/1826 Derechos de autor 2018 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Rico, Yessica Reyes Estanislao, Leticia Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico |
| title | Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico |
| title_full | Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico |
| title_fullStr | Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico |
| title_full_unstemmed | Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico |
| title_short | Pollen viability and germinability of putative Bursera hybrids (section Bullockia; Burseraceae) in Mexico |
| title_sort | pollen viability and germinability of putative bursera hybrids (section bullockia; burseraceae) in mexico |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1435 |
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