Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection
Background and Aims: Soybean regeneration and transformation are considered highly genotype-specific; however, little is known about desirable seed traits that could be useful indicators of their regeneration and transformation capacities. In the present study, eight commercially available Mexican s...
| Huvudupphovsmän: | , , |
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| Materialtyp: | Online |
| Språk: | eng |
| Publicerad: |
Instituto de Ecología, A.C.
2018
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| Länkar: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1421 |
| _version_ | 1799771347207847936 |
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| author | Mora Vásquez, Soledad García-Lara, Silverio Cardineau, Guy A. |
| author_facet | Mora Vásquez, Soledad García-Lara, Silverio Cardineau, Guy A. |
| author_sort | Mora Vásquez, Soledad |
| collection | ABM |
| description | Background and Aims: Soybean regeneration and transformation are considered highly genotype-specific; however, little is known about desirable seed traits that could be useful indicators of their regeneration and transformation capacities. In the present study, eight commercially available Mexican soybean varieties, as well as the Jack genotype as a control, were phenotypically characterized to determine the seed traits that are important factors for their in vitro performance and susceptibility to Agrobacterium tumefaciens infection.Methods: Grain dimensions, seed weight, moisture, oil, protein, ash, carbohydrate content and macro and micro elements (Mg, K, Ca, P, Na, Mn, Fe, Cu and Zn) were measured and correlated with the corresponding direct shoot organogenesis capability and Agrobacterium infection susceptibility using the cotyledonary node as explant.Key results: Notably, the ash content was the only important factor that inversely correlated with the capacity for shoot organogenesis, whereas carbohydrate and phosphorus content were positively correlated with susceptibility to Agrobacterium infection. The best response in terms of multiple shoot formation and Agrobacterium transient transformation was observed with the soybean varieties Huasteca-100, Nainari and Suaqui-86, which have lower ash content and a higher carbohydrate and phosphorus content.Conclusions: In most reported studies, seed phenotypic traits have been overlooked as factors that influence their regeneration and transformation potential. In the present study, we present evidence of associations between some specific seed traits and regeneration and transient transformation of soybean. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1421 |
| institution | Acta Botanica Mexicana |
| language | eng |
| publishDate | 2018 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-14212022-11-25T21:32:53Z Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection Características fenotípicas de semillas de soya mexicana y su correlación con la inducción de brotes in vitro y con la susceptibilidad de infección por Agrobacterium Mora Vásquez, Soledad García-Lara, Silverio Cardineau, Guy A. cotyledonary node Glycine max phenotypic characterization regeneration soybean genotypes transient transformation caracterización fenotípica genotipos de soya Glycine max nodo cotiledonario regeneración transformación transitoria Background and Aims: Soybean regeneration and transformation are considered highly genotype-specific; however, little is known about desirable seed traits that could be useful indicators of their regeneration and transformation capacities. In the present study, eight commercially available Mexican soybean varieties, as well as the Jack genotype as a control, were phenotypically characterized to determine the seed traits that are important factors for their in vitro performance and susceptibility to Agrobacterium tumefaciens infection.Methods: Grain dimensions, seed weight, moisture, oil, protein, ash, carbohydrate content and macro and micro elements (Mg, K, Ca, P, Na, Mn, Fe, Cu and Zn) were measured and correlated with the corresponding direct shoot organogenesis capability and Agrobacterium infection susceptibility using the cotyledonary node as explant.Key results: Notably, the ash content was the only important factor that inversely correlated with the capacity for shoot organogenesis, whereas carbohydrate and phosphorus content were positively correlated with susceptibility to Agrobacterium infection. The best response in terms of multiple shoot formation and Agrobacterium transient transformation was observed with the soybean varieties Huasteca-100, Nainari and Suaqui-86, which have lower ash content and a higher carbohydrate and phosphorus content.Conclusions: In most reported studies, seed phenotypic traits have been overlooked as factors that influence their regeneration and transformation potential. In the present study, we present evidence of associations between some specific seed traits and regeneration and transient transformation of soybean. Antecedentes y Objetivos: La regeneración y transformación de soya son consideradas altamente genotipo-específicas; sin embargo, muy poco se sabe acerca de rasgos deseables de la semilla que pudieran ser indicadores útiles de sus capacidades de regeneración y transformación. En el presente estudio, ocho variedades de soya mexicanas comercialmente disponibles, así como el genotipo Jack como control, se caracterizaron fenotípicamente con el fin de determinar qué características de la semilla son factores importantes para su desempeño in vitro y su susceptibilidad de infección por Agrobacterium tumefaciens.Métodos: Se midieron las dimensiones del grano, el peso de semilla, contenido de humedad, aceite, proteína, cenizas, carbohidratos, así como el contenido de macro y microelementos (Mg, K, Ca, P, Na, Mn, Fe, Cu y Zn) y se correlacionaron con la correspondiente capacidad de organogénesis directa y susceptibilidad de infección por Agrobacterium, usando el nodo cotiledonario como explante.Resultados clave: Notablemente, el contenido de cenizas fue el único factor importante que se correlacionó inversamente con la capacidad de organogénesis, mientras que los contenidos de carbohidratos y fósforo se correlacionaron positivamente con la susceptibilidad de infección por Agrobacterium. La mejor respuesta en términos de formación de brotes múltiples y transformación transitoria con Agrobacterium se observó con las variedades de soya Huasteca-100, Nainari y Suaqui-86, las cuales tienen un menor contenido de cenizas y un mayor contenido de carbohidratos y fósforo.Conclusiones: En la gran mayoría de estudios reportados, se han pasado por alto los rasgos fenotípicos de las semillas como posibles factores que influyen en su potencial de regeneración y transformación. En el presente estudio, presentamos evidencia de asociaciones entre algunos rasgos específicos de las semillas con la regeneración y la transformación transitoria de soya. Instituto de Ecología, A.C. 2018-12-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf application/epub+zip application/xml https://abm.ojs.inecol.mx/index.php/abm/article/view/1421 10.21829/abm126.2019.1421 Acta Botanica Mexicana; No. 126 (2019) Acta Botanica Mexicana; Núm. 126 (2019) 2448-7589 0187-7151 eng https://abm.ojs.inecol.mx/index.php/abm/article/view/1421/4000 https://abm.ojs.inecol.mx/index.php/abm/article/view/1421/4001 https://abm.ojs.inecol.mx/index.php/abm/article/view/1421/1783 Derechos de autor 2018 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Mora Vásquez, Soledad García-Lara, Silverio Cardineau, Guy A. Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection |
| title | Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection |
| title_full | Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection |
| title_fullStr | Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection |
| title_full_unstemmed | Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection |
| title_short | Phenotypic traits of Mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to Agrobacterium infection |
| title_sort | phenotypic traits of mexican soybean seeds and their correlation with in vitro shoot induction and susceptibility to agrobacterium infection |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1421 |
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