Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico
Background and Aims: Phosphorus deficiency in crops is generally remediated through the application of fertilizers. However, this practice is costly and deteriorates the environment. The use of biofertilizers such as phosphate-solubilizing fungi is a promising alternative. The objective of the prese...
| Những tác giả chính: | , , |
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| Định dạng: | Online |
| Ngôn ngữ: | spa |
| Được phát hành: |
Instituto de Ecología, A.C.
2018
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| Truy cập trực tuyến: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1390 |
| _version_ | 1799771342840528896 |
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| author | Romero Fernández, Abraham de Jesus Arias Mota, Rosa María Mendoza Villarreal, Rosalinda |
| author_facet | Romero Fernández, Abraham de Jesus Arias Mota, Rosa María Mendoza Villarreal, Rosalinda |
| author_sort | Romero Fernández, Abraham de Jesus |
| collection | ABM |
| description | Background and Aims: Phosphorus deficiency in crops is generally remediated through the application of fertilizers. However, this practice is costly and deteriorates the environment. The use of biofertilizers such as phosphate-solubilizing fungi is a promising alternative. The objective of the present study was to isolate and select native phosphate-solubilizing soil fungi from Coahuila state, Mexico, with potential to be used as biofertilizers.Methods: Fungal strains were isolated using the soil-particle washing technique. The selection of phosphate-solubilizing strains was performed in a Sundara medium with tricalcium phosphate, and phosphate-solubilizing capacity was visually detected based on the presence of hyaline halos. The qualitative evaluation was carried out by calculating the solubilization index. In the quantitative evaluation, the cultures were incubated for 25 days in liquid medium, and soluble P, pH, and fungal biomass were measured every five days.Key results: A total of 271 strains of fungi were isolated. Of these isolates, 52.76% tested positive for phosphate solubilization in the qualitative evaluation. With regard to solubilization efficiency, the values ranged from 2.06-6.85. The strains Sp. Mz54, Aspergillus sp. 4C19, Penicillium sp. Mu73, Penicillium sp. Mu77, and Penicillium sp. A56 presented the highest solubilization efficiency indices (t<0.005). In the quantitative evaluation, the strain Penicillium sp. Mu73 solubilized the highest quantity of phosphorus (83.26 mg l-1) (t<0.005). According to the regression analyses, the phosphorus concentration increased as the pH decreased (r2=0.81).Conclusions: The strain Penicillium sp. Mu73 has high potential for use as a biofertilizer because it solubilizes a high amount of phosphorus under in vitro conditions. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1390 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2018 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-13902022-11-17T22:06:44Z Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico Aislamiento y selección de hongos de suelo solubilizadores de fósforo nativos del estado de Coahuila, México Romero Fernández, Abraham de Jesus Arias Mota, Rosa María Mendoza Villarreal, Rosalinda biofertilizers filamentous fungi tricalcium phosphate biofertilizantes fosfato tricálcico hongos filamentosos Background and Aims: Phosphorus deficiency in crops is generally remediated through the application of fertilizers. However, this practice is costly and deteriorates the environment. The use of biofertilizers such as phosphate-solubilizing fungi is a promising alternative. The objective of the present study was to isolate and select native phosphate-solubilizing soil fungi from Coahuila state, Mexico, with potential to be used as biofertilizers.Methods: Fungal strains were isolated using the soil-particle washing technique. The selection of phosphate-solubilizing strains was performed in a Sundara medium with tricalcium phosphate, and phosphate-solubilizing capacity was visually detected based on the presence of hyaline halos. The qualitative evaluation was carried out by calculating the solubilization index. In the quantitative evaluation, the cultures were incubated for 25 days in liquid medium, and soluble P, pH, and fungal biomass were measured every five days.Key results: A total of 271 strains of fungi were isolated. Of these isolates, 52.76% tested positive for phosphate solubilization in the qualitative evaluation. With regard to solubilization efficiency, the values ranged from 2.06-6.85. The strains Sp. Mz54, Aspergillus sp. 4C19, Penicillium sp. Mu73, Penicillium sp. Mu77, and Penicillium sp. A56 presented the highest solubilization efficiency indices (t<0.005). In the quantitative evaluation, the strain Penicillium sp. Mu73 solubilized the highest quantity of phosphorus (83.26 mg l-1) (t<0.005). According to the regression analyses, the phosphorus concentration increased as the pH decreased (r2=0.81).Conclusions: The strain Penicillium sp. Mu73 has high potential for use as a biofertilizer because it solubilizes a high amount of phosphorus under in vitro conditions. Antecedentes y Objetivos: La deficiencia de fósforo en los cultivos agrícolas generalmente es atendida mediante la aplicación de fertilizantes. Sin embargo, estas prácticas resultan costosas y causan deterioro al medio ambiente. El uso de bio-productos como los hongos solubilizadores de fósforo constituyen una alternativa promisoria. El objetivo de este trabajo fue aislar y seleccionar hongos del suelo fosfato solubilizadores nativos de Coahuila, México, con potencial para ser utilizados como biofertilizantes.Métodos: El aislamiento de los hongos se llevó a cabo mediante la técnica de filtración de partículas de suelo. La selección de las cepas se realizó en el medio de cultivo de Sundara con fosfato tricálcico; la capacidad fosfato solubilizadora se detectó visualmente mediante la presencia de halos hialinos en el medio de cultivo. La evaluación cualitativa se determinó mediante el índice de eficiencia de solubilización. En la evaluación cuantitativa, los cultivos se incubaron en medio líquido por 25 días y cada cinco se midió el P soluble, pH y biomasa fúngica.Resultados clave: Se obtuvo un total de 271 aislamientos de hongos; de éstos 52.76% proyectaron resultados positivos a la prueba cualitativa de solubilización de fósforo. En cuanto a la eficiencia de solubilización, ésta fluctuó entre 2.06-6.85 unidades. Las cepas Sp. Mz54, Aspergillus sp. 4C19, Penicillium sp. Mu73, Penicillium sp. Mu77 y Penicillium sp. A56 presentaron los mayores índices de eficiencia de solubilización (t<0.005). En la medición cuantitativa, la cepa Penicillium sp. Mu73 solubilizó la mayor cantidad de fósforo (83.26 mg l-1) (t<0.005). De acuerdo con los análisis de regresión, el fósforo aumentó a medida que el pH decreció (r2=0.81).Conclusiones: La cepa Penicillium sp. Mu73 tiene un alto potencial para ser utilizada como biofertilizante ya que promueve un alto contenido de fósforo soluble en condiciones in vitro. Instituto de Ecología, A.C. 2018-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf application/epub+zip application/xml https://abm.ojs.inecol.mx/index.php/abm/article/view/1390 10.21829/abm126.2019.1390 Acta Botanica Mexicana; No. 126 (2019) Acta Botanica Mexicana; Núm. 126 (2019) 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1390/3994 https://abm.ojs.inecol.mx/index.php/abm/article/view/1390/3995 https://abm.ojs.inecol.mx/index.php/abm/article/view/1390/1781 Derechos de autor 2018 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Romero Fernández, Abraham de Jesus Arias Mota, Rosa María Mendoza Villarreal, Rosalinda Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico |
| title | Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico |
| title_full | Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico |
| title_fullStr | Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico |
| title_full_unstemmed | Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico |
| title_short | Isolation and selection of solubilizing phosphate soil fungi native to Coahuila state, Mexico |
| title_sort | isolation and selection of solubilizing phosphate soil fungi native to coahuila state, mexico |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1390 |
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