Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers

Background and Aims: Mango (Mangifera indica) belongs to the Anacardiaceae family and is native to South Asia. It has currently reached a large distribution because of its development in warm climates, and its adaptation to a wide range of environmental conditions. Knowledge of the population geneti...

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Huvudupphovsmän: Guerra, Martha, Ruiz, Rosalba, Pardo Pérez, Enrique
Materialtyp: Online
Språk:spa
Publicerad: Instituto de Ecología, A.C. 2018
Länkar:https://abm.ojs.inecol.mx/index.php/abm/article/view/1285
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author Guerra, Martha
Ruiz, Rosalba
Pardo Pérez, Enrique
author_facet Guerra, Martha
Ruiz, Rosalba
Pardo Pérez, Enrique
author_sort Guerra, Martha
collection ABM
description Background and Aims: Mango (Mangifera indica) belongs to the Anacardiaceae family and is native to South Asia. It has currently reached a large distribution because of its development in warm climates, and its adaptation to a wide range of environmental conditions. Knowledge of the population genetic diversity of mango germplasm in Valencia, Córdoba, Colombia, would allow for selection of promising mango varieties and populations for use in the country's breeding programs. The aim of the present work was to determine the genetic diversity of a population of Mangifera indica in Valencia using 12 microsatellite markers.Methods: The study was conducted with leaves of Mangifera indica collected in the city of Valencia and dehydrated with silica gel. The analysis of individuals was performed using 12 microsatellite molecular markers. Employing different software (GENALEX, CERVUS, FSTAT and MEGA 7), were determined: number of alleles, effective number of alleles, heterozygosity observed and expected, genetic distance, Hardy-Weinberg equilibrium, polymorphic information content, fixation indices FIS, FIT, FST and a dendrogram.Key results: All microsatellites used were polymorphic. Between 5 to 12 alleles were detected, with an average of 7 alleles per locus and a total of 84. The average effective number of alleles was 4.551. PIC values ranged between 0.86 and 0.49 for MiIIHR23 and MiIIHR34 respectively. The Hardy-Weinberg test indicated that the population was in disequilibrium (p<0.05) for the 12 markers. The fixation index revealed an excess of homozygotes. The average of the observed and expected heterozygosity was 0.355 and 0.748 respectively.Conclusions: The analyzed population presented high genetic diversity and the markers were very informative, attending the PIC.
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spelling oai:oai.abm.ojs.inecol.mx:article-12852022-11-17T22:46:29Z Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers Diversidad genética de Mangifera indica (Anacardiaceae) en Valencia, Córdoba, Colombia, usando marcadores microsatélites Guerra, Martha Ruiz, Rosalba Pardo Pérez, Enrique alleles distance genetics Hardy-Weinberg equilibrium polymorphic information content alelos contenido de información polimórfica distancia genética equilibrio de Hardy-Weinberg Background and Aims: Mango (Mangifera indica) belongs to the Anacardiaceae family and is native to South Asia. It has currently reached a large distribution because of its development in warm climates, and its adaptation to a wide range of environmental conditions. Knowledge of the population genetic diversity of mango germplasm in Valencia, Córdoba, Colombia, would allow for selection of promising mango varieties and populations for use in the country's breeding programs. The aim of the present work was to determine the genetic diversity of a population of Mangifera indica in Valencia using 12 microsatellite markers.Methods: The study was conducted with leaves of Mangifera indica collected in the city of Valencia and dehydrated with silica gel. The analysis of individuals was performed using 12 microsatellite molecular markers. Employing different software (GENALEX, CERVUS, FSTAT and MEGA 7), were determined: number of alleles, effective number of alleles, heterozygosity observed and expected, genetic distance, Hardy-Weinberg equilibrium, polymorphic information content, fixation indices FIS, FIT, FST and a dendrogram.Key results: All microsatellites used were polymorphic. Between 5 to 12 alleles were detected, with an average of 7 alleles per locus and a total of 84. The average effective number of alleles was 4.551. PIC values ranged between 0.86 and 0.49 for MiIIHR23 and MiIIHR34 respectively. The Hardy-Weinberg test indicated that the population was in disequilibrium (p<0.05) for the 12 markers. The fixation index revealed an excess of homozygotes. The average of the observed and expected heterozygosity was 0.355 and 0.748 respectively.Conclusions: The analyzed population presented high genetic diversity and the markers were very informative, attending the PIC. Antecedentes y Objetivos: El mango (Mangifera indica) pertenece a la familia Anacardiaceae y es nativa del sur de Asia. Actualmente ha alcanzado una gran distribución por su desarrollo en climas cálidos, y su adaptación a una amplia gama de condiciones ambientales. El conocimiento de la diversidad genético poblacional del germoplasma de mango en Valencia, Córdoba, Colombia, permitiría seleccionar variedades y poblaciones promisorias para emplearse en los programas de mejoramiento genético del país. El objetivo del presente trabajo fue determinar la diversidad genética de una población de Mangifera indica en Valencia empleando 12 marcadores microsatélites.Métodos: El estudio se realizó con hojas de Mangifera indica colectadas en el municipio de Valencia deshidratadas con silica gel. El análisis de los individuos se realizó utilizando 12 marcadores moleculares microsatélites. Empleando diferente software (GENALEX, CERVUS, FSTAT y MEGA 7) se determinó: número de alelos, número efectivo de alelos, heterocigosidad observada y esperada, distancia genética y equilibrio de Hardy-Weinberg, contenido de información polimórfica, índices de fijación FIS, FIT y FST, y un dendrograma.Resultados clave: Todos los microsatélites analizados fueron polimórficos. Se detectaron entre 5 y 12 alelos, con un promedio de 7 alelos por locus y un total de 84. El número efectivo de alelos promedio fue 4.551. Los valores del PIC oscilaron entre 0.86 y 0.49 para los marcadores MiIIHR23 y MiIIHR34 respectivamente. La prueba de Hardy-Weinberg indicó que la población estaba en desequilibrio (p<0.05) para los 12 marcadores. El índice de fijación reveló un exceso de homocigotos. El promedio de heterocigosidad, observada y esperada, fue de 0.355 y 0.748 respectivamente.Conclusiones: La población analizada presentó alta diversidad genética y los marcadores resultaron muy informativos, atendiendo al PIC. Instituto de Ecología, A.C. 2018-05-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/html https://abm.ojs.inecol.mx/index.php/abm/article/view/1285 10.21829/abm124.2018.1285 Acta Botanica Mexicana; No. 124 (2018); 105-116 Acta Botanica Mexicana; Núm. 124 (2018); 105-116 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1285/pdf_1 https://abm.ojs.inecol.mx/index.php/abm/article/view/1285/html Derechos de autor 2018 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0
spellingShingle Guerra, Martha
Ruiz, Rosalba
Pardo Pérez, Enrique
Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers
title Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers
title_full Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers
title_fullStr Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers
title_full_unstemmed Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers
title_short Genetic diversity of Mangifera indica (Anacardiaceae) in Valencia, Córdoba, Colombia, using microsatellite markers
title_sort genetic diversity of mangifera indica (anacardiaceae) in valencia, córdoba, colombia, using microsatellite markers
url https://abm.ojs.inecol.mx/index.php/abm/article/view/1285
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