Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae)
Background and Aims: Meliaceae species are extremely recalcitrant during germination and in vitro processes. Therefore, this research focuses on characterization and optimization of a highly efficient system by secondary somatic embryogenesis in Azadirachta indica, which is an important step for enh...
| Main Authors: | , |
|---|---|
| Format: | Online |
| Sprog: | eng |
| Udgivet: |
Instituto de Ecología, A.C.
2018
|
| Online adgang: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1242 |
| _version_ | 1799771326526783488 |
|---|---|
| author | Artigas Ramirez, Maria Daniela Fernandez Da Silva, Rafael |
| author_facet | Artigas Ramirez, Maria Daniela Fernandez Da Silva, Rafael |
| author_sort | Artigas Ramirez, Maria Daniela |
| collection | ABM |
| description | Background and Aims: Meliaceae species are extremely recalcitrant during germination and in vitro processes. Therefore, this research focuses on characterization and optimization of a highly efficient system by secondary somatic embryogenesis in Azadirachta indica, which is an important step for enhancing secondary metabolite production and regeneration in recalcitrant species.Material and Methods: Leaf and cotyledon sections were induced in MS medium supplemented with benzylaminopurine (BAP) alone, or combined with 1-naphthaleneacetic acid (NAA) and, abscisic acid (BA) with thidiazuron (TDZ).Key results: Azadirachta indica developed primary somatic embryos with BAP. Shoot and root formation occurred at low concentrations of BAP, while somatic embryogenesis was favored under high levels of BAP or TDZ. Primary and secondary somatic embryos were evidenced continuously and asynchronously. The highest amount of somatic embryos was obtained with cytokinins. However, the concentration might be significant to differentiate between primary and secondary embryos. Moreover, the auxins are key for inducing histodifferentiation in embryos. Shoot induction occurred after transfer of the embryos to hormone-free MS medium. The shoots were rooted in MS1/2.Conclusions: The secondary somatic embryos were distinguished and characterized during the whole process and the efficient system was established with cotyledon sections at short term, which offers several advantages such as the production of metabolites. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1242 |
| institution | Acta Botanica Mexicana |
| language | eng |
| publishDate | 2018 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-12422022-11-17T22:54:15Z Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) Caracterización morfo-anatómica de la embriogénesis somática secundaria en Azadirachta indica (Meliaceae) Artigas Ramirez, Maria Daniela Fernandez Da Silva, Rafael abscisic acid (BA) asynchronous embryogenesis benzylaminopurine (BAP) cyclic somatic embryogenesis thidiazuron (TDZ) ácido abscísico (BA) bencil-aminopurina (BAP) embriogénesis asincrónica embriogénesis somática cíclica tidiazuron (TDZ) Background and Aims: Meliaceae species are extremely recalcitrant during germination and in vitro processes. Therefore, this research focuses on characterization and optimization of a highly efficient system by secondary somatic embryogenesis in Azadirachta indica, which is an important step for enhancing secondary metabolite production and regeneration in recalcitrant species.Material and Methods: Leaf and cotyledon sections were induced in MS medium supplemented with benzylaminopurine (BAP) alone, or combined with 1-naphthaleneacetic acid (NAA) and, abscisic acid (BA) with thidiazuron (TDZ).Key results: Azadirachta indica developed primary somatic embryos with BAP. Shoot and root formation occurred at low concentrations of BAP, while somatic embryogenesis was favored under high levels of BAP or TDZ. Primary and secondary somatic embryos were evidenced continuously and asynchronously. The highest amount of somatic embryos was obtained with cytokinins. However, the concentration might be significant to differentiate between primary and secondary embryos. Moreover, the auxins are key for inducing histodifferentiation in embryos. Shoot induction occurred after transfer of the embryos to hormone-free MS medium. The shoots were rooted in MS1/2.Conclusions: The secondary somatic embryos were distinguished and characterized during the whole process and the efficient system was established with cotyledon sections at short term, which offers several advantages such as the production of metabolites. Antecedentes y Objetivos: Las especies de Meliaceae son extremadamente recalcitrantes durante la germinación y en los procesos in vitro. Por lo tanto, este estudio está enfocado en caracterizar y optimizar un sistema altamente eficiente de propagación a través de embriogénesis somática secundaria en Azadirachta indica, paso importante para la producción de metabolitos secundarios, la propagación o regeneración de estas especies recalcitrantes.Materiales y Métodos: Las secciones de hojas y cotiledones fueron inducidos en medio MS suplementado con bencil-aminopurina (BAP) sola o combinada con ácido 1-naftalenacético (NAA) y ácido abscísico (BA) con tidiazuron (TDZ).Resultados clave: Azadirachta indica desarrolla embriones somáticos primarios con BAP. La formación de tallos y raíces ocurre en bajas concentraciones de BAP, mientras la embriogénesis somática es favorecida a altos niveles de BAP o TDZ. Los embriones somáticos primarios y secundarios fueron evidenciados continuamente y sincrónicamente o asincrónicamente. Una gran cantidad de embriones somáticos fueron obtenidos con citoquininas. Sin embargo, la concentración tiene un rol importante en la diferenciación entre embriones primarios y secundarios. Además, las auxinas son la clave para inducir la histo-diferenciación en los embriones. La inducción de tallo ocurrió después de que los embriones somáticos se transfirieron a un medio libre de hormonas (MS). Estos tallos fueron enraizados con MS1/2.Conclusiones: Los embriones somáticos secundarios fueron distinguidos y caracterizados durante todo el proceso y el sistema fue establecido con segmentos de cotiledones en corto tiempo; esto ofrece oportunidades ventajosas como la producción de metabolitos. Instituto de Ecología, A.C. 2018-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/html https://abm.ojs.inecol.mx/index.php/abm/article/view/1242 10.21829/abm122.2018.1242 Acta Botanica Mexicana; No. 122 (2018); 7-20 Acta Botanica Mexicana; Núm. 122 (2018); 7-20 2448-7589 0187-7151 eng https://abm.ojs.inecol.mx/index.php/abm/article/view/1242/pdf https://abm.ojs.inecol.mx/index.php/abm/article/view/1242/html Derechos de autor 2018 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Artigas Ramirez, Maria Daniela Fernandez Da Silva, Rafael Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) |
| title | Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) |
| title_full | Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) |
| title_fullStr | Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) |
| title_full_unstemmed | Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) |
| title_short | Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae) |
| title_sort | morpho-anatomical characterization of secondary somatic embryogenesis in azadirachta indica (meliaceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1242 |
| work_keys_str_mv | AT artigasramirezmariadaniela morphoanatomicalcharacterizationofsecondarysomaticembryogenesisinazadirachtaindicameliaceae AT fernandezdasilvarafael morphoanatomicalcharacterizationofsecondarysomaticembryogenesisinazadirachtaindicameliaceae AT artigasramirezmariadaniela caracterizacionmorfoanatomicadelaembriogenesissomaticasecundariaenazadirachtaindicameliaceae AT fernandezdasilvarafael caracterizacionmorfoanatomicadelaembriogenesissomaticasecundariaenazadirachtaindicameliaceae |
