Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico
Background and Aims: The colonization of lithic substrates by fungal communities is determined by the properties of the substrate (bioreceptivity) and climatic and microclimatic conditions. However, the effect of the exposure time of the limestone surface to the environment on fungal communities has...
| Auteurs principaux: | , , , , |
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| Format: | Online |
| Langue: | eng |
| Publié: |
Instituto de Ecología, A.C.
2016
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| Accès en ligne: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1168 |
| _version_ | 1799771317133639680 |
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| author | Gómez-Cornelio, Sergio Ortega-Morales, Otto Morón-Ríos, Alejandro Reyes-Estebanez, Manuela de la Rosa-García, Susana |
| author_facet | Gómez-Cornelio, Sergio Ortega-Morales, Otto Morón-Ríos, Alejandro Reyes-Estebanez, Manuela de la Rosa-García, Susana |
| author_sort | Gómez-Cornelio, Sergio |
| collection | ABM |
| description | Background and Aims: The colonization of lithic substrates by fungal communities is determined by the properties of the substrate (bioreceptivity) and climatic and microclimatic conditions. However, the effect of the exposure time of the limestone surface to the environment on fungal communities has not been extensively investigated. In this study, we analyze the composition and structure of fungal communities occurring in biofilms on limestone walls of modern edifications constructed at different times in a subtropical environment in Campeche, Mexico.Methods: A chronosequence of walls built one, five and 10 years ago was considered. On each wall, three surface areas of 3 × 3 cm of the corresponding biofilm were scraped for subsequent analysis. Fungi were isolated by washing and particle filtration technique and were then inoculated in two contrasting culture media (oligotrophic and copiotrophic). The fungi were identified according to macro and microscopic characteristics.Key results: We found 73 genera and 202 species from 844 isolates. Our results showed that fungal communities differed in each biofilm. In the middle-aged biofilm a high number of isolates was found, but both species richness and diversity were low. In contrast, in the old biofilm species richness and diversity were high; Hyphomycete 1, Myrothecium roridum and Pestalotiopsis maculans were abundant. The dominant species in the middle-aged biofilm were Curvularia lunata, Curvularia pallescens, Fusarium oxysporum and Fusarium redolens, and in the young biofilm were Cladosporium cladosporioides, Curvularia clavata, Paraconiothyrium sp. and Phoma eupyrena.Conclusions: Our results suggest that the composition of the fungal community in each biofilm varies according to time of exposure to the environment. Furthermore, the fungal community was composed of a pool of uncommon species that might be autochthonous to limestone. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1168 |
| institution | Acta Botanica Mexicana |
| language | eng |
| publishDate | 2016 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-11682022-12-23T04:40:00Z Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico Cambios en la composición de la comunidad fúngica de biopelículas sobre roca calcárea a través de una cronosecuencia en Campeche, México Gómez-Cornelio, Sergio Ortega-Morales, Otto Morón-Ríos, Alejandro Reyes-Estebanez, Manuela de la Rosa-García, Susana dominant species fungal colonization fungal diversity succession trophic preference colonización fúngica diversidad fúngica especies dominantes preferencia trófica sucesión Background and Aims: The colonization of lithic substrates by fungal communities is determined by the properties of the substrate (bioreceptivity) and climatic and microclimatic conditions. However, the effect of the exposure time of the limestone surface to the environment on fungal communities has not been extensively investigated. In this study, we analyze the composition and structure of fungal communities occurring in biofilms on limestone walls of modern edifications constructed at different times in a subtropical environment in Campeche, Mexico.Methods: A chronosequence of walls built one, five and 10 years ago was considered. On each wall, three surface areas of 3 × 3 cm of the corresponding biofilm were scraped for subsequent analysis. Fungi were isolated by washing and particle filtration technique and were then inoculated in two contrasting culture media (oligotrophic and copiotrophic). The fungi were identified according to macro and microscopic characteristics.Key results: We found 73 genera and 202 species from 844 isolates. Our results showed that fungal communities differed in each biofilm. In the middle-aged biofilm a high number of isolates was found, but both species richness and diversity were low. In contrast, in the old biofilm species richness and diversity were high; Hyphomycete 1, Myrothecium roridum and Pestalotiopsis maculans were abundant. The dominant species in the middle-aged biofilm were Curvularia lunata, Curvularia pallescens, Fusarium oxysporum and Fusarium redolens, and in the young biofilm were Cladosporium cladosporioides, Curvularia clavata, Paraconiothyrium sp. and Phoma eupyrena.Conclusions: Our results suggest that the composition of the fungal community in each biofilm varies according to time of exposure to the environment. Furthermore, the fungal community was composed of a pool of uncommon species that might be autochthonous to limestone. Antecedentes y Objetivos: La colonización de los sustratos líticos por comunidades fúngicas está determinada por las propiedades del sustrato (bioreceptividad) y las condiciones climáticas y microclimáticas. Sin embargo, los efectos del tiempo de exposición de la superficie de la roca calcárea al ambiente sobre la composición de las comunidades fúngicas no se ha investigado. En este estudio, analizamos la composición y estructura de las comunidades fúngicas inmersas en biopelículas asociadas a roca calcárea, en paredes de edificaciones modernas construidas a diferentes tiempos en un ambiente subtropical en Campeche, México.Métodos: Se consideró una cronosecuencia de paredes construidas a uno, cinco y 10 años. Sobre cada pared, se rasparon tres superficies de 3 × 3 cm para cada biopelícula. Los hongos se aislaron por la técnica de lavado y filtración de partículas, posteriormente se inocularon en dos medios de cultivo contrastantes (un medio oligotrófico y uno copiotrófico). Los hongos se identificaron de acuerdo a sus características macro y microscópicas.Resultados clave: Encontramos 73 géneros y 202 especies de 844 aislados. Los resultados mostraron que las comunidades fúngicas son diferentes en las tres biopelículas. En la biopelícula de desarrollo intermedio encontramos un alto número de aislados, pero tanto la riqueza como la diversidad fueron bajas. En contraste, en la biopelícula avanzada, los valores de riqueza de especies y diversidad fueron altos, y las especies abundantes fueron Hyphomycete 1, Myrothecium roridum y Pestalotiopsis maculans. Las especies dominantes en la biopelícula intermedia fueron Curvularia lunata, Curvularia pallescens, Fusarium oxysporum y Fusarium redolens, y en la biopelícula joven fueron Cladosporium cladosporioides, Curvularia clavata, Paraconiothyrium sp. y Phoma eupyrena.Conclusiones: Nuestros resultados sugieren que la composición de la comunidad fúngica en cada biopelícula cambia de acuerdo al tiempo de exposición de la roca calcárea al ambiente. Además, como parte de la composición de la comunidad fúngica, encontramos un conjunto de especies poco comunes que podrían ser autóctonas en la roca calcárea. Instituto de Ecología, A.C. 2016-10-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/html https://abm.ojs.inecol.mx/index.php/abm/article/view/1168 10.21829/abm117.2016.1168 Acta Botanica Mexicana; No. 117 (2016); 59-77 Acta Botanica Mexicana; Núm. 117 (2016); 59-77 2448-7589 0187-7151 eng https://abm.ojs.inecol.mx/index.php/abm/article/view/1168/1381 https://abm.ojs.inecol.mx/index.php/abm/article/view/1168/html Derechos de autor 2016 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Gómez-Cornelio, Sergio Ortega-Morales, Otto Morón-Ríos, Alejandro Reyes-Estebanez, Manuela de la Rosa-García, Susana Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico |
| title | Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico |
| title_full | Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico |
| title_fullStr | Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico |
| title_full_unstemmed | Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico |
| title_short | Changes in fungal community composition of biofilms on limestone across a chronosequence in Campeche, Mexico |
| title_sort | changes in fungal community composition of biofilms on limestone across a chronosequence in campeche, mexico |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1168 |
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