Extraction and embryo morphology of Bursera bipinnata (Burseraceae)
Background and Aims: The understanding of the reproductive biology of plants, from the formation of the seed to its establishment, allows the development of different conservation strategies. In the genus Bursera, whose main center of diversification is Mexico, the diaspore is a pyrene formed by a s...
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| Format: | Online |
| Sprache: | spa |
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Instituto de Ecología, A.C.
2016
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| Online Zugang: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1165 |
| _version_ | 1799771316216135680 |
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| author | Ramos-Ordoñez, María Felix del Coro Arizmendi, María Flores-Enríquez, Valeria Márquez-Guzmán, Judith |
| author_facet | Ramos-Ordoñez, María Felix del Coro Arizmendi, María Flores-Enríquez, Valeria Márquez-Guzmán, Judith |
| author_sort | Ramos-Ordoñez, María Felix |
| collection | ABM |
| description | Background and Aims: The understanding of the reproductive biology of plants, from the formation of the seed to its establishment, allows the development of different conservation strategies. In the genus Bursera, whose main center of diversification is Mexico, the diaspore is a pyrene formed by a strongly lignified endocarp enclosing the seed. The anatomical characteristics of the pyrene and the parthenocarpy are the main factors that have hindered the study of embryogenesis and the development of propagation techniques. In this paper we describe the morphology of the Bursera bipinnata embryo, extracted by a technique that does not alter the structure or the chemical composition of tissues.Methods: During the beginning of the dispersal stage 2014, we collected unripe fruits and pyrenes with exposed pseudaril. Unripe fruits were fixed in FAA. A low speed micromotor with handpiece was used. A conical burr was used in unripe fruits to scabble the fruit valves. To open the pyrenes a diamond disc was used. After opening the endocarp, embryos were removed and fixed.Key results: The pattern of development coincides with other model species of angiosperms. In immature fruits globular, heart-shaped, torpedo and cotyledonary stages were observed, the latter in early stages. During the torpedo stage, cotyledons branch into three lobes and are spliced, as they grow, they are folded upon themselves, and cover the embryonic axis; however, the radicular meristem is always exposed.Conclusions: The extraction technique is rapid, inexpensive and non-destructive to tissues, having a potential use in the detailed analysis of embryogenesis in the genus Bursera, tissue culture and ex situ conservation. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1165 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2016 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-11652022-12-23T04:40:05Z Extraction and embryo morphology of Bursera bipinnata (Burseraceae) Extracción y morfología del embrión de Bursera bipinnata (Burseraceae) Ramos-Ordoñez, María Felix del Coro Arizmendi, María Flores-Enríquez, Valeria Márquez-Guzmán, Judith Burseraceae cotyledon embryogenesis extraction technique pyrene seed Burseraceae cotiledón embriogénesis pireno tecnica de extracción semilla Background and Aims: The understanding of the reproductive biology of plants, from the formation of the seed to its establishment, allows the development of different conservation strategies. In the genus Bursera, whose main center of diversification is Mexico, the diaspore is a pyrene formed by a strongly lignified endocarp enclosing the seed. The anatomical characteristics of the pyrene and the parthenocarpy are the main factors that have hindered the study of embryogenesis and the development of propagation techniques. In this paper we describe the morphology of the Bursera bipinnata embryo, extracted by a technique that does not alter the structure or the chemical composition of tissues.Methods: During the beginning of the dispersal stage 2014, we collected unripe fruits and pyrenes with exposed pseudaril. Unripe fruits were fixed in FAA. A low speed micromotor with handpiece was used. A conical burr was used in unripe fruits to scabble the fruit valves. To open the pyrenes a diamond disc was used. After opening the endocarp, embryos were removed and fixed.Key results: The pattern of development coincides with other model species of angiosperms. In immature fruits globular, heart-shaped, torpedo and cotyledonary stages were observed, the latter in early stages. During the torpedo stage, cotyledons branch into three lobes and are spliced, as they grow, they are folded upon themselves, and cover the embryonic axis; however, the radicular meristem is always exposed.Conclusions: The extraction technique is rapid, inexpensive and non-destructive to tissues, having a potential use in the detailed analysis of embryogenesis in the genus Bursera, tissue culture and ex situ conservation. Antecedentes y Objetivos: El entendimiento de la biología reproductiva de las plantas, desde la formación de la semilla hasta su establecimiento, permite el desarrollo de diferentes estrategias de conservación. En el género Bursera, cuyo principal centro de diversificación es México, la diáspora es un pireno formado por un endocarpo fuertemente lignificado que encierra a la semilla. Las características anatómicas del pireno y la partenocarpia son los principales factores que han obstaculizado el estudio de la embriogénesis y el desarrollo de técnicas de propagación. En este trabajo se describe la morfología del embrión de Bursera bipinnata extraído mediante una técnica que no altera la estructura o la composición química de los tejidos.Métodos: Durante el inicio de la etapa de dispersión 2014, se colectaron frutos inmaduros y pirenos con pseudoarilo expuesto, los frutos inmaduros se fijaron en FAA. Se utilizó un micromotor con pieza de mano de baja velocidad. En los frutos inmaduros se utilizó un fresón cónico para desgastar las valvas del fruto. Para abrir los pirenos se utilizó un disco de diamante. Una vez abierto el endocarpo, los embriones se extrajeron y se fijaron.Resultados clave: El patrón de desarrollo coincide con otras especies modelo de angiospermas. En los frutos inmaduros se encontraron las fases globular, acorazonada, torpedo y cotiledonar, ésta última en etapas iniciales. En la fase de torpedo los cotiledones se ramifican en tres lóbulos y se empalman, a medida que crecen se pliegan sobre si mismos encerrando el eje embrionario; sin embargo, el meristemo radicular siempre queda expuesto. El embrión se clasificó como embrión axial foliado contortuplicado.Conclusiones: La técnica de extracción es rápida, de bajo costo y no invasiva para los tejidos, teniendo un uso potencial en el análisis detallado de la embriogénesis del género Bursera, el cultivo de tejidos y la conservación ex situ. Instituto de Ecología, A.C. 2016-10-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf text/html https://abm.ojs.inecol.mx/index.php/abm/article/view/1165 10.21829/abm117.2016.1165 Acta Botanica Mexicana; No. 117 (2016); 27-35 Acta Botanica Mexicana; Núm. 117 (2016); 27-35 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1165/1378 https://abm.ojs.inecol.mx/index.php/abm/article/view/1165/html Derechos de autor 2016 Acta Botanica Mexicana |
| spellingShingle | Ramos-Ordoñez, María Felix del Coro Arizmendi, María Flores-Enríquez, Valeria Márquez-Guzmán, Judith Extraction and embryo morphology of Bursera bipinnata (Burseraceae) |
| title | Extraction and embryo morphology of Bursera bipinnata (Burseraceae) |
| title_full | Extraction and embryo morphology of Bursera bipinnata (Burseraceae) |
| title_fullStr | Extraction and embryo morphology of Bursera bipinnata (Burseraceae) |
| title_full_unstemmed | Extraction and embryo morphology of Bursera bipinnata (Burseraceae) |
| title_short | Extraction and embryo morphology of Bursera bipinnata (Burseraceae) |
| title_sort | extraction and embryo morphology of bursera bipinnata (burseraceae) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1165 |
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