Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser)
Patterns of meiotic chromosome pairing were compared between three sunflower plants: cultivated sunflower (Helianthus annuus L. var. macrocarpus, public line HA 89), wild sunflower (Helianthus annuus L. ssp. texanus Heiser, collected in Saltillo, Coah.), and the F1 hybrid. The study was carried out...
| Päätekijät: | , , |
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| Aineistotyyppi: | Online |
| Kieli: | spa |
| Julkaistu: |
Instituto de Ecología, A.C.
2007
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| Linkit: | https://abm.ojs.inecol.mx/index.php/abm/article/view/1043 |
| _version_ | 1799771271899119616 |
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| author | Rodríguez de la Paz, Jesús Gómez Martínez, Martha Reyes Valdés, M. Humberto |
| author_facet | Rodríguez de la Paz, Jesús Gómez Martínez, Martha Reyes Valdés, M. Humberto |
| author_sort | Rodríguez de la Paz, Jesús |
| collection | ABM |
| description | Patterns of meiotic chromosome pairing were compared between three sunflower plants: cultivated sunflower (Helianthus annuus L. var. macrocarpus, public line HA 89), wild sunflower (Helianthus annuus L. ssp. texanus Heiser, collected in Saltillo, Coah.), and the F1 hybrid. The study was carried out through analysis of meiocytes in diakinesis and metaphase I, based on the meiotic configuration frequency. Pollen viability was evaluated by means of a staining method. Chromosome pairing was normal in the three materials, with absence of univalents and multivalents, and only chain and ring bivalents were observed, therefore the parental genomes are highly compatible in meiosis. The hybrid showed a chromosome pairing index of 0.82, close to the midparent value (0.80), and the differences between the hybrid and each parental taxon (cultivated 0.87, and wild 0.75) were highly significant. Besides, this index was variable within populations. This indicates that the chiasma number is under multigenic control and is affected by the environment. Thus, a population formed with both progenitors could be used to analyze the quantitative trait loci (QTLs) for meiotic chromosome pairing. The pollen viability in the hybrid (92.58%) was similar to that found in the parental taxa (wild 95.57%, and cultivated 95.33%). The pollen viability of the F1 was high, it showed fertility, and the parental genomes were highly compatible in meiosis, confirming that there is no barrier for the sexual reproduction, and that gene flow and transference of desirables characters from wild to cultivated H. annuus are easily attainable. |
| format | Online |
| id | oai:oai.abm.ojs.inecol.mx:article-1043 |
| institution | Acta Botanica Mexicana |
| language | spa |
| publishDate | 2007 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | oai:oai.abm.ojs.inecol.mx:article-10432022-12-23T05:51:36Z Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) Análisis meiótico de una cruza entre girasol cultivado (Helianthus annuus L. var. macrocarpus) y girasol silvestre (Helianthus annuus L. ssp. texanus Heiser) Rodríguez de la Paz, Jesús Gómez Martínez, Martha Reyes Valdés, M. Humberto Helianthus annuus hybridization meiotic analysis meiotic configurations análisis meiótico configuraciones meióticas Helianthus annuus hibridación Patterns of meiotic chromosome pairing were compared between three sunflower plants: cultivated sunflower (Helianthus annuus L. var. macrocarpus, public line HA 89), wild sunflower (Helianthus annuus L. ssp. texanus Heiser, collected in Saltillo, Coah.), and the F1 hybrid. The study was carried out through analysis of meiocytes in diakinesis and metaphase I, based on the meiotic configuration frequency. Pollen viability was evaluated by means of a staining method. Chromosome pairing was normal in the three materials, with absence of univalents and multivalents, and only chain and ring bivalents were observed, therefore the parental genomes are highly compatible in meiosis. The hybrid showed a chromosome pairing index of 0.82, close to the midparent value (0.80), and the differences between the hybrid and each parental taxon (cultivated 0.87, and wild 0.75) were highly significant. Besides, this index was variable within populations. This indicates that the chiasma number is under multigenic control and is affected by the environment. Thus, a population formed with both progenitors could be used to analyze the quantitative trait loci (QTLs) for meiotic chromosome pairing. The pollen viability in the hybrid (92.58%) was similar to that found in the parental taxa (wild 95.57%, and cultivated 95.33%). The pollen viability of the F1 was high, it showed fertility, and the parental genomes were highly compatible in meiosis, confirming that there is no barrier for the sexual reproduction, and that gene flow and transference of desirables characters from wild to cultivated H. annuus are easily attainable. En el presente trabajo se compararon los patrones de apareamiento cromosómico meiótico del girasol cultivado (Helianthus annuus L. var. macrocarpus, línea pública HA 89), girasol silvestre (Helianthus annuus L. ssp. texanus Heiser, procedente de Saltillo, Coahuila) y del híbrido F1. Para ello se analizaron meiocitos en diacinesis y metafase I, basándose en la frecuencia de las configuraciones meióticas. Se evaluó la viabilidad de polen por medio de un método de tinción. El apareamiento cromosómico fue normal en los tres genotipos, con ausencia de univalentes y multivalentes, y sólo se observaron bivalentes en cadena y anillo, por lo que se concluye que los genomas parentales son altamente compatibles en la meiosis. El híbrido presentó un índice de apareamiento cromosómico de 0.82, próximo al valor medio parental (0.80) y la diferencia del híbrido con cada uno de los taxa parentales (taxon cultivado 0.87 y taxon silvestre 0.75) fue altamente significativa, además de variar dentro de las poblaciones estudiadas. Esto indica que el carácter número de quiasmas está bajo control multigénico y se ve afectado por el ambiente. Así pues, una población formada con los progenitores podría ser utilizada para analizar los loci de atributos cuantitativos (QTLs) para apareamiento cromosómico meiótico. El híbrido tuvo un porcentaje de viabilidad de polen (92.58) comparable con el de las plantas parentales (la silvestre 95.57 y la cultivada 95.33). La viabilidad de polen en la F1 fue alta, mostró fertilidad, y los genomas de los progenitores fueron altamente compatibles en la meiosis, corroborando que no hay barreras para la reproducción sexual. Esto indica que no hay dificultad en el flujo genético y que se pueden transferir caracteres deseables de H. annuus silvestre al cultivado. Instituto de Ecología, A.C. 2007-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo evaluado por pares application/pdf https://abm.ojs.inecol.mx/index.php/abm/article/view/1043 10.21829/abm80.2007.1043 Acta Botanica Mexicana; No. 80 (2007); 7-20 Acta Botanica Mexicana; Núm. 80 (2007); 7-20 2448-7589 0187-7151 spa https://abm.ojs.inecol.mx/index.php/abm/article/view/1043/1204 Derechos de autor 2016 Acta Botanica Mexicana https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Rodríguez de la Paz, Jesús Gómez Martínez, Martha Reyes Valdés, M. Humberto Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) |
| title | Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) |
| title_full | Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) |
| title_fullStr | Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) |
| title_full_unstemmed | Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) |
| title_short | Meiotic analysis of a cross between cultivated sunflower (Helianthus annuus L. var. macrocarpus) and wild sunflower (Helianthus annuus L. ssp. texanus Heiser) |
| title_sort | meiotic analysis of a cross between cultivated sunflower (helianthus annuus l. var. macrocarpus) and wild sunflower (helianthus annuus l. ssp. texanus heiser) |
| url | https://abm.ojs.inecol.mx/index.php/abm/article/view/1043 |
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