Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae)
A methodological process is described here to solve two bottle necks that render the establishment of fruit flies under artificial conditions for mass production difficult. The first was the selection of an oviposition system and the second the adaptation to the artificial diet used for larval devel...
| Hlavní autoři: | , , |
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| Médium: | Online |
| Jazyk: | spa |
| Vydáno: |
Instituto de Ecología, A.C.
2012
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| On-line přístup: | https://azm.ojs.inecol.mx/index.php/azm/article/view/836 |
| _version_ | 1799770119517241344 |
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| author | Flores, Hector S. Hernández, Emilio Toledo, Jorge |
| author_facet | Flores, Hector S. Hernández, Emilio Toledo, Jorge |
| author_sort | Flores, Hector S. |
| collection | AZM |
| description | A methodological process is described here to solve two bottle necks that render the establishment of fruit flies under artificial conditions for mass production difficult. The first was the selection of an oviposition system and the second the adaptation to the artificial diet used for larval development. Females preferred to oviposit on yellow spheres, and on white organza cloth flat panels covered with transparent silicon. A larval diet used to rear A.ludens, allowed the highest larval development, survival from egg to larva was 26.9%, larval weight 19.6mg, pupation at 24 h 97.7%, pupal weight 16.7mg and adult emergence 83.3%. Survival curves (nx, 1x) and life expectancy (ex) indicated at least 50% of the adults survive ~40 days. Life expectancy was of 34.74 days. The reproductive rate was 345.38 daughters/mother/generation; the intrinsic rate of population growth was 0.35days-1; while the median generation time and the time of duplication were 16.82 and 20.2 days, respectively. Moreover, we discuss results based on quality control parameters of insects after of 16 generations in mass-rearing under artificial conditions. |
| format | Online |
| id | azm-article-836 |
| institution | Acta Zoológica Mexicana |
| language | spa |
| publishDate | 2012 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | azm-article-8362022-05-24T22:55:20Z Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) Desarrollo de un Sistema de Cría Artificial para Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) Flores, Hector S. Hernández, Emilio Toledo, Jorge Mass-rearing larval diet South American fruit fly artificial egg collection Cría de insectos dieta larvaria mosca sudamericana de la fruta sustrato artificial para colecta de huevo A methodological process is described here to solve two bottle necks that render the establishment of fruit flies under artificial conditions for mass production difficult. The first was the selection of an oviposition system and the second the adaptation to the artificial diet used for larval development. Females preferred to oviposit on yellow spheres, and on white organza cloth flat panels covered with transparent silicon. A larval diet used to rear A.ludens, allowed the highest larval development, survival from egg to larva was 26.9%, larval weight 19.6mg, pupation at 24 h 97.7%, pupal weight 16.7mg and adult emergence 83.3%. Survival curves (nx, 1x) and life expectancy (ex) indicated at least 50% of the adults survive ~40 days. Life expectancy was of 34.74 days. The reproductive rate was 345.38 daughters/mother/generation; the intrinsic rate of population growth was 0.35days-1; while the median generation time and the time of duplication were 16.82 and 20.2 days, respectively. Moreover, we discuss results based on quality control parameters of insects after of 16 generations in mass-rearing under artificial conditions. Se describe el proceso metodológico para solucionar dos cuellos de botella que dificultan el establecimiento de la cría artificial de Anastrepha fraterculus a gran escala. Primero, la selección del sistema de oviposición y segundo la adaptación a la dieta artificial utilizada para el desarrollo larvario. Las hembras tuvieron una mayor preferencia para ovipositar en esferas de color amarillo y en paneles planos elaborados con tela tipo organza de color blanco cubiertos con una película de silicón transparente. Después de evaluar las dietas para el desarrollo larvario de cinco especies de moscas de la fruta, la dieta con la que se obtuvieron los rendimientos más altos correspondió a la dieta ALU (Anastrephaludens), con la cual la supervivencia de huevo a larva fue del 26.9%, peso de larva de 19.6 mg, porcentaje de pupación a las 24 h 97.7%, peso de pupa 16.7 mg y emergencia de adultos de 83.3%. El análisis de las curvas de supervivencia (nx, lx) y la estimación de la expectativa de vida (ex) indicaron que al menos el 50% de los individuos vivieron ~40 días. La expectativa de vida del adulto al momento de la emergencia fue de 38.74 días. La tasa neta de reproducción fue de 345.38 hijas/madre/generación, la tasa intrínseca de crecimiento poblacional fue de 0.35 días-1; mientras que el tiempo medio de generación y el tiempo de duplicación de la población fue de 16.82 y de 20.2 días, respectivamente. Asimismo, se discuten valores de los parámetros de calidad registrados en los insectos luego de 16 generaciones bajocondiciones de cría artificial. Instituto de Ecología, A.C. 2012-08-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original articles Artículos originales application/pdf https://azm.ojs.inecol.mx/index.php/azm/article/view/836 10.21829/azm.2012.282836 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 28 No. 2 (2012); 321-340 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 28 Núm. 2 (2012); 321-340 2448-8445 0065-1737 spa https://azm.ojs.inecol.mx/index.php/azm/article/view/836/1003 Derechos de autor 2012 ACTA ZOOLÓGICA MEXICANA (N.S.) http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Flores, Hector S. Hernández, Emilio Toledo, Jorge Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) |
| title | Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) |
| title_full | Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) |
| title_fullStr | Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) |
| title_full_unstemmed | Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) |
| title_short | Development of an artificial mass-rearing method for Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) |
| title_sort | development of an artificial mass-rearing method for anastrepha fraterculus (wied.) (diptera: tephritidae) |
| url | https://azm.ojs.inecol.mx/index.php/azm/article/view/836 |
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