Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores
We optimize several aspects involved in the method for a reliable, more simple, and easy obtaining of bile acids (AB) and cholesterol (Co) from the feces of wild carnivores, including: selection of the optimal developer; adaptation of the mobile phase in a first chromatographic evaluation; optimizat...
| Hauptverfasser: | , , , , , , , |
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| Format: | Online |
| Sprache: | spa |
| Veröffentlicht: |
Instituto de Ecología, A.C.
2012
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| Online Zugang: | https://azm.ojs.inecol.mx/index.php/azm/article/view/835 |
| _version_ | 1799770119224688640 |
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| author | Salame-Méndez, Arturo Andrade-Herrera, Moisés Zamora-Torres, Leo Serrano, Héctor Soto-Mendoza, Saúl Castro-Campillo, Alondra Ramírez Pulido, José Haro-Castellanos, Jorge |
| author_facet | Salame-Méndez, Arturo Andrade-Herrera, Moisés Zamora-Torres, Leo Serrano, Héctor Soto-Mendoza, Saúl Castro-Campillo, Alondra Ramírez Pulido, José Haro-Castellanos, Jorge |
| author_sort | Salame-Méndez, Arturo |
| collection | AZM |
| description | We optimize several aspects involved in the method for a reliable, more simple, and easy obtaining of bile acids (AB) and cholesterol (Co) from the feces of wild carnivores, including: selection of the optimal developer; adaptation of the mobile phase in a first chromatographic evaluation; optimization of separation through a second chromatographic evaluation with another mobile phase; the use of minimal amounts of both fecal sample (mg) or the ethanolic solution (uL) in which the former were preserved. The new procedure is simpler and easily reproducible, and thus it turns out to be an analytic, non-invasive, and feasible tool for the identification of wild carnivores by means of the AB within their feces. In order to probe the method, we analyzed feces from Puma concolor, Panthera onca, Canis lupus baileyi, and Canis latrans maintained in captivity, which are also described and, therefore, we also present for the first time, the profile of AB for the Mexican wolf. |
| format | Online |
| id | azm-article-835 |
| institution | Acta Zoológica Mexicana |
| language | spa |
| publishDate | 2012 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | azm-article-8352022-05-24T22:25:28Z Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores Método optimizado para evaluar ácidos biliares de muestras fecales secas o preservadas en etanol como herramienta para identificar carnívoros silvestres Salame-Méndez, Arturo Andrade-Herrera, Moisés Zamora-Torres, Leo Serrano, Héctor Soto-Mendoza, Saúl Castro-Campillo, Alondra Ramírez Pulido, José Haro-Castellanos, Jorge Carnivores bile acids feces thin layer chromatography identification non-invasive methods Puma Panthera Canis Carnívoros ácidos biliares heces fecales cromatografía en capa fina identificación métodos no-invasivos Puma Panthera Canis We optimize several aspects involved in the method for a reliable, more simple, and easy obtaining of bile acids (AB) and cholesterol (Co) from the feces of wild carnivores, including: selection of the optimal developer; adaptation of the mobile phase in a first chromatographic evaluation; optimization of separation through a second chromatographic evaluation with another mobile phase; the use of minimal amounts of both fecal sample (mg) or the ethanolic solution (uL) in which the former were preserved. The new procedure is simpler and easily reproducible, and thus it turns out to be an analytic, non-invasive, and feasible tool for the identification of wild carnivores by means of the AB within their feces. In order to probe the method, we analyzed feces from Puma concolor, Panthera onca, Canis lupus baileyi, and Canis latrans maintained in captivity, which are also described and, therefore, we also present for the first time, the profile of AB for the Mexican wolf. Se optimizan varios aspectos involucrados en el método para una obtención confiable, más sencilla y expedita de ácidos biliares (AB) y colesterol (Co), a partir de excretas de carnívoros silvestres, incluyendo: la selección de un revelador óptimo; la adecuación de la fase móvil en una primera evaluación cromatográfica; la optimización de la separación, mediante una segunda evaluación cromatográfica con su propia fase móvil; el uso de cantidades mínimas de muestra fecal (mg); así como de la solución etanólica (uL) en donde fueron preservadas como materia prima. El nuevo método es más sencillo y fácilmente reproducible, por lo que resulta en una herramienta analítica indirecta, no-invasiva y sumamente viable para la identificación de carnívoros silvestres, a partir de los AB en sus excretas. Para probar el método se usaron excretas de Puma concolor, Panthera onca, Canis lupus baileyi y Canis latrans mantenidos en cautiverio, las cuales se describen, por lo que adicionalmente, en este trabajo se presenta por primera vez el perfil de AB en el lobo mexicano. Instituto de Ecología, A.C. 2012-08-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original articles Artículos originales application/pdf https://azm.ojs.inecol.mx/index.php/azm/article/view/835 10.21829/azm.2012.282835 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 28 No. 2 (2012); 305-320 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 28 Núm. 2 (2012); 305-320 2448-8445 0065-1737 spa https://azm.ojs.inecol.mx/index.php/azm/article/view/835/1002 Derechos de autor 2012 ACTA ZOOLÓGICA MEXICANA (N.S.) http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Salame-Méndez, Arturo Andrade-Herrera, Moisés Zamora-Torres, Leo Serrano, Héctor Soto-Mendoza, Saúl Castro-Campillo, Alondra Ramírez Pulido, José Haro-Castellanos, Jorge Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| title | Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| title_full | Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| title_fullStr | Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| title_full_unstemmed | Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| title_short | Optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| title_sort | optimized method for evaluation of bile acids from dry or ethanol preserved fecal samples as a tool for the identification of wild carnivores |
| url | https://azm.ojs.inecol.mx/index.php/azm/article/view/835 |
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