Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions
In order to develop biorrational fruit fly control methods, the activity of two strains of Beauveria bassiana (Bb26 and BbJLSV) and a strain of Metarhizium anisopliae (MaCENGICAÑA) against adults of Anastrepha obliqua was evaluated in the laboratory. The Bb26 strain caused the highest infection rate...
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| Format: | Online |
| Language: | spa |
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Instituto de Ecología, A.C.
2010
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| Online Access: | https://azm.ojs.inecol.mx/index.php/azm/article/view/796 |
| _version_ | 1799770105556500480 |
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| author | Díaz-Ordaz, Noé Hernández Pérez, Nelson Toledo, Jorge |
| author_facet | Díaz-Ordaz, Noé Hernández Pérez, Nelson Toledo, Jorge |
| author_sort | Díaz-Ordaz, Noé Hernández |
| collection | AZM |
| description | In order to develop biorrational fruit fly control methods, the activity of two strains of Beauveria bassiana (Bb26 and BbJLSV) and a strain of Metarhizium anisopliae (MaCENGICAÑA) against adults of Anastrepha obliqua was evaluated in the laboratory. The Bb26 strain caused the highest infection rate (99.8%), followed by the BbJLSV strain (93.5%), and the MaCENGICAÑA strain which caused the lowest infection (89.8%). Both males and females were susceptible to the strains tested, with a similar mortality in both sexes. The median lethal time (LT50) was 3.9, 5.3 and 6.4 days for MaCENGICAÑA, Bb26 and BbJLSV, respectively, with an average median lethal time for the three strains of 5.2 days. Results indicate that the most pathogenic strain against adults of A. obliqua was Bb26, with a median lethal concentration (LC50) equivalent to 3.8 x 106 conidia ml-1, followed by the MaCENGICAÑA strain equivalent to 4.8 x 106 conidia ml-1 and the BbJLSV strain with an equivalent to 8.8 x 106 conidia ml-1. The Bb26 strain produced the highest sporulation, with a concentration of 1.52 x 108 conidia ml-1, and a sporulation of 77.9% of infected adults; followed by the MaCENGICAÑA strain (1.50 x108 conidia ml-1) and BbJLSV (1.76 x108 conidia ml-1) with 73.8 and 72.6% of sporulation, respectively. Sporulation of infected cadavers and inoculum concentration showed a positive correlation between the applied concentration and the production of conidia by flies (R2 = 0.91, Y = 1.63 + 0.14x), which suggested that when increasing the concentration of inoculum, the production of conidia tended to increase. |
| format | Online |
| id | azm-article-796 |
| institution | Acta Zoológica Mexicana |
| language | spa |
| publishDate | 2010 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | azm-article-7962022-09-02T23:50:33Z Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions Patogenicidad de tres cepas de hongos entomopatógenos a adultos de Anastrepha obliqua (Macquart) (Diptera: Tephritidae) en condiciones de laboratorio. Díaz-Ordaz, Noé Hernández Pérez, Nelson Toledo, Jorge Microbial control entomopathogen Anastrepha obliqua fruit flies Control microbiano entomopatógeno Anastrepha obliqua moscas de la fruta. In order to develop biorrational fruit fly control methods, the activity of two strains of Beauveria bassiana (Bb26 and BbJLSV) and a strain of Metarhizium anisopliae (MaCENGICAÑA) against adults of Anastrepha obliqua was evaluated in the laboratory. The Bb26 strain caused the highest infection rate (99.8%), followed by the BbJLSV strain (93.5%), and the MaCENGICAÑA strain which caused the lowest infection (89.8%). Both males and females were susceptible to the strains tested, with a similar mortality in both sexes. The median lethal time (LT50) was 3.9, 5.3 and 6.4 days for MaCENGICAÑA, Bb26 and BbJLSV, respectively, with an average median lethal time for the three strains of 5.2 days. Results indicate that the most pathogenic strain against adults of A. obliqua was Bb26, with a median lethal concentration (LC50) equivalent to 3.8 x 106 conidia ml-1, followed by the MaCENGICAÑA strain equivalent to 4.8 x 106 conidia ml-1 and the BbJLSV strain with an equivalent to 8.8 x 106 conidia ml-1. The Bb26 strain produced the highest sporulation, with a concentration of 1.52 x 108 conidia ml-1, and a sporulation of 77.9% of infected adults; followed by the MaCENGICAÑA strain (1.50 x108 conidia ml-1) and BbJLSV (1.76 x108 conidia ml-1) with 73.8 and 72.6% of sporulation, respectively. Sporulation of infected cadavers and inoculum concentration showed a positive correlation between the applied concentration and the production of conidia by flies (R2 = 0.91, Y = 1.63 + 0.14x), which suggested that when increasing the concentration of inoculum, the production of conidia tended to increase. Buscando alternativas biorracionales para el control de moscas de la fruta, se evaluó la actividad de dos cepas del hongo entomopatógeno Beauveria bassiana (Bb26 y BbJLSV) y una de Metarhizium anisopliae (MaCENGICAÑA) contra adultos de Anastrepha obliqua en laboratorio. La cepa Bb26 fue la que ocasionó mayor infección (99.8%), seguida por la cepa BbJLSV (93.5%), y de la cepa MaCENGICAÑA (89.8%). Tanto hembras como machos fueron susceptibles a la infección por hongos de las cepas evaluadas, observándose una mortalidad similar para adultos de ambos sexos. La cepa MaCENGICAÑA provoco mortalidad con el menor tiempo letal medio (TL50 = 3.9 días); seguida por la cepa Bb26 (5.3 días) y la cepa BbJLSV (6.4 días). El tiempo letal medio promedio para el conjunto de las tres cepas fue de 5.2 días. Los resultados indican que la cepa más patogénica contra adultos fue la Bb26, con una concentración letal media (CL50) de 3.8 x 106 conidios ml-1, seguida por la cepa MaCENGICAÑA con una CL50 equivalente a 4.8 x 106 conidios ml-1 y la cepa BbJLSV con una CL50 equivalente a 8.8 x 106 conidios ml-1. La cepa Bb26 fue la que produjo mayor cantidad de conidios, ya que las moscas infectadas que fueron tratadas con una concentración de 1.52 x 108 conidios ml-1 registraron 77.9% de esporulación; seguida de las cepas MaCENGICAÑA (1.50 x108 conidios ml-1) y BbJLSV (1.76 x108 conidios ml-1) que registraron 73.8 y 72.6% de esporulación, respectivamente. La esporulación de los cadáveres infestados registró una correlación positiva entre la concentración aplicada y la producción de conidios (R2 = 0.91, Y = 1.63 + 0.14x), lo cual indicó que conforme se incrementó la concentración para tratar las moscas, fue mayor la producción de conidios en las moscas infectadas. Instituto de Ecología, A.C. 2010-12-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original articles Artículos originales application/pdf https://azm.ojs.inecol.mx/index.php/azm/article/view/796 10.21829/azm.2010.263796 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 26 No. 3 (2010); 481-494 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 26 Núm. 3 (2010); 481-494 2448-8445 0065-1737 spa https://azm.ojs.inecol.mx/index.php/azm/article/view/796/964 Derechos de autor 2010 ACTA ZOOLÓGICA MEXICANA (N.S.) http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Díaz-Ordaz, Noé Hernández Pérez, Nelson Toledo, Jorge Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions |
| title | Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions |
| title_full | Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions |
| title_fullStr | Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions |
| title_full_unstemmed | Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions |
| title_short | Pathogenecity of three strains of entomopathogenic fungus on Anastrepha obliqua adults (Macquart) (Diptera: Tephritidae) under laboratory conditions |
| title_sort | pathogenecity of three strains of entomopathogenic fungus on anastrepha obliqua adults (macquart) (diptera: tephritidae) under laboratory conditions |
| url | https://azm.ojs.inecol.mx/index.php/azm/article/view/796 |
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