Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems
The knowledge on the presence and abundance of natural enemies of white grubs in Mexico is limited. Therefore the aim of the present work is to isolate, identify and preserve entomopathogens from corn cultivated soils and from collections made directly on white grubs in Morelos. Altitudes of these g...
| Autori principali: | , , , , |
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| Natura: | Online |
| Lingua: | spa |
| Pubblicazione: |
Instituto de Ecología, A.C.
2011
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| Accesso online: | https://azm.ojs.inecol.mx/index.php/azm/article/view/777 |
| _version_ | 1799770099673989120 |
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| author | Hernández-Velázquez, V. Manuel Cervantes Espíndola, Zito Villalobos, Francisco J. Lina García, Laura Peña Chora, Guadalupe |
| author_facet | Hernández-Velázquez, V. Manuel Cervantes Espíndola, Zito Villalobos, Francisco J. Lina García, Laura Peña Chora, Guadalupe |
| author_sort | Hernández-Velázquez, V. Manuel |
| collection | AZM |
| description | The knowledge on the presence and abundance of natural enemies of white grubs in Mexico is limited. Therefore the aim of the present work is to isolate, identify and preserve entomopathogens from corn cultivated soils and from collections made directly on white grubs in Morelos. Altitudes of these georeferenced (GPS) sites ranged between 1000 to 2300 masl. Forty four soil sample collectionswere made in these sites. Fungal detection was made by the trap insect technique using Galleria mellonella (Lepidoptera: Pyralidae) as a bait. Besides, white grub species present in each site belonging to different larval instars. These larvae were identified to morphospecies on the basis of the raster palidia and were observed until death or adult emergence. Field collected dead larvae with fungal infections were also identified in the same way. Both the larvae of G. mellonela and white grubs were used to isolate and identify the fugal species present. Fungal identification was made on the basis of morphology and fungal development on the insect which correlates with conidial size and shape and the conidial formation in colonies of purified isolates. Fourty eight fungal isolates (seven from white grubs and 41 G. mellonella) have been preserved in the Biological Control Laboratory of the CEIB-UAEM (a replicate has been placed in Fungal Entomopathogens Colllection of CNRCB). In this collection 15 strains of Metarhizium anisopliae, 30 strains of Beauveria bassiana and three strains of Paecilomyces sp. are present. This fungal diversity is valuable in the zone for conservation purposes and for its potencial as biocontrol agents against white grubs and other corn pests. |
| format | Online |
| id | azm-article-777 |
| institution | Acta Zoológica Mexicana |
| language | spa |
| publishDate | 2011 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | azm-article-7772017-10-12T04:37:57Z Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems Aislamiento de hongos entomopatógenos en suelo y sobre gallinas ciegas (Coleoptera: Melolonthidae) en agroecosistemas de maíz Hernández-Velázquez, V. Manuel Cervantes Espíndola, Zito Villalobos, Francisco J. Lina García, Laura Peña Chora, Guadalupe withe grubs corn Beauveria bassiana Metarhizium anisopliae entomopathogenic fungi gallina ciega morfoespecies maíz Galleria mellonella Beauveria bassiana Metarhizium anisopliae The knowledge on the presence and abundance of natural enemies of white grubs in Mexico is limited. Therefore the aim of the present work is to isolate, identify and preserve entomopathogens from corn cultivated soils and from collections made directly on white grubs in Morelos. Altitudes of these georeferenced (GPS) sites ranged between 1000 to 2300 masl. Forty four soil sample collectionswere made in these sites. Fungal detection was made by the trap insect technique using Galleria mellonella (Lepidoptera: Pyralidae) as a bait. Besides, white grub species present in each site belonging to different larval instars. These larvae were identified to morphospecies on the basis of the raster palidia and were observed until death or adult emergence. Field collected dead larvae with fungal infections were also identified in the same way. Both the larvae of G. mellonela and white grubs were used to isolate and identify the fugal species present. Fungal identification was made on the basis of morphology and fungal development on the insect which correlates with conidial size and shape and the conidial formation in colonies of purified isolates. Fourty eight fungal isolates (seven from white grubs and 41 G. mellonella) have been preserved in the Biological Control Laboratory of the CEIB-UAEM (a replicate has been placed in Fungal Entomopathogens Colllection of CNRCB). In this collection 15 strains of Metarhizium anisopliae, 30 strains of Beauveria bassiana and three strains of Paecilomyces sp. are present. This fungal diversity is valuable in the zone for conservation purposes and for its potencial as biocontrol agents against white grubs and other corn pests. En México se conoce poco sobre la presencia y abundancia de enemigos naturales de plagas suelo como las Phyllophaga spp. Por lo anterior se realizó el presente trabajo con los objetivos de aislar, identificar y conservar entomopatógenos en suelos cultivados con maíz y sobre inmaduros de gallina ciega en el estado de Morelos. En altitudes de 1000 a 2300 msnm, se realizaron cuarenta y cuatro colectas de suelo en sitios georeferenciados (GPS). La detección de hongos se realizó mediante la técnica de insecto trampa, Galleria mellonella (Lepidoptera: Pyralidae). Además, se colectaron inmaduros de gallina ciega de diversos estadios larvales en diferentes localidades, se separaron por morfoespecie de acuerdo a la forma del ráster y se tomaron fotografías de cada morfoespecie. Tanto de las larvas de G. mellonela como de las gallinas ciegas se realizaron aislamientos e identificación de hongos entomopatógenos. Se identificaron siete morfoespecies y tres especies de Phyllophaga, alimentándose sobre raíces de plantas de maíz, además de tres especies de Paranomala. Se conservan en la Colección del Laboratorio de Control Biológico del Centro de Investigación en Biotecnología de la Universidad Autónoma del Estado de Morelos (con respaldo en la Colección de Hongos Entomopatógenos del CNRCB) cuarenta y ocho aislamientos de hongos entomopatógenos (siete aislados de gallina ciega y 41 de larvas de G. mellonella). De éstos, 15 corresponden a Metarhizium anisopliae, 30 a Beauveria bassiana y tres a Paecilomyces sp. Estos aislamientos poseen un gran valor para la zona, desde el punto de vista de conservación de la biodiversidad de enemigos naturales de plagas agrícolas y por su potencial como agentes de control biológico de GC y otras plagas del maíz. Instituto de Ecología, A.C. 2011-12-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original articles Artículos originales application/pdf https://azm.ojs.inecol.mx/index.php/azm/article/view/777 10.21829/azm.2011.273777 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 27 No. 3 (2011) ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 27 Núm. 3 (2011) 2448-8445 0065-1737 spa https://azm.ojs.inecol.mx/index.php/azm/article/view/777/944 Derechos de autor 2011 ACTA ZOOLÓGICA MEXICANA (N.S.) |
| spellingShingle | Hernández-Velázquez, V. Manuel Cervantes Espíndola, Zito Villalobos, Francisco J. Lina García, Laura Peña Chora, Guadalupe Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems |
| title | Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems |
| title_full | Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems |
| title_fullStr | Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems |
| title_full_unstemmed | Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems |
| title_short | Entomopathogenic fungi isolated from soil and immature white grubs (Coleoptera: Melolonthidae) in corn agrosystems |
| title_sort | entomopathogenic fungi isolated from soil and immature white grubs (coleoptera: melolonthidae) in corn agrosystems |
| url | https://azm.ojs.inecol.mx/index.php/azm/article/view/777 |
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