| Shrnutí: | This contribution has two objectives: 1. to publish some new records of mammal species for Guanajuato and 2. to mention additional localities for mammal species that have been registered before in the state, increasing their distribution. Specimens were colected from 2003 to 2006 in different sites of “Sierra Gorda de Guanajuato” Biosphere Reserve and were deposited at Mammal scientific collection at Escuela Nacional de Ciencias Biológicas, I.P.N. We included also some specimens collected some years ago and were in the same collection. We present 11 new registers and 14 species that extend their range in Guanajuato, represented by 364 specimen within 5 families, 14 genera and 25 species (20 rodents and 5 bats). Three species of rodents are endemic to Mexico (Peromyscus difficilis, P. levipes y P. melanophrys). About bats, Desmodus rotundus had not been registered for Guanajuato and Leptonycteris yerbabuenae is the only species with a conservation status. The 25 species of this manuscript complete and actualize knowledge about mammalian biodiversity, plus 73 species previously published for the state we have a total of 98 species registered in Guanajuato.
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