Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos
We evaluated the presence of Batrachochytrium dendrobatidis (Bd) in 13 Mexican amphibians associated to abiotic and biotic factors as a species distribution model by a classification and regression tree (CART). Chytrid fungus was diagnosed from cutaneous swabs in adults’ amphibians and oral swabs of...
| Main Authors: | , , |
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| Format: | Online |
| Language: | spa |
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Instituto de Ecología, A.C.
2019
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| Online Access: | https://azm.ojs.inecol.mx/index.php/azm/article/view/2066 |
| _version_ | 1799770369349910528 |
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| author | García-Feria, Luis Manuel Brousset, Dulce María Cervantes-Olivares, Roberto Arnulfo |
| author_facet | García-Feria, Luis Manuel Brousset, Dulce María Cervantes-Olivares, Roberto Arnulfo |
| author_sort | García-Feria, Luis Manuel |
| collection | AZM |
| description | We evaluated the presence of Batrachochytrium dendrobatidis (Bd) in 13 Mexican amphibians associated to abiotic and biotic factors as a species distribution model by a classification and regression tree (CART). Chytrid fungus was diagnosed from cutaneous swabs in adults’ amphibians and oral swabs of tadpoles by nested PCR for a 300bp rRNA fragment. Specimens were sampled in seven vegetation types, during dry and rainy seasons. The chytrid fungus was detected in Charadrayla taeniopus and Leptodactylus fragilis, species that were not previously recorded with the fungus; the Bd also was confirmed on amphibians that inhabit from perennial rainforest at southeastern Mexico. According to CARTs, host species and precipitation were the most important factor to Bd occurrence but their combination with other variables as development stage, environmental temperature, season, vegetation type and aquatic life index have influence on its presence. In addition, the tadpoles had 1.52 times more prevalence of Bd than adults, and that tadpoles from two amphibian species studied could be reservoirs potentially. Although no amphibian showed clinical signs of chytridiomycosis, our findings indicate that the presence and persistence of Bd may be related with species susceptibility or resistance and interaction of diverse abiotic and biotic factors. |
| format | Online |
| id | azm-article-2066 |
| institution | Acta Zoológica Mexicana |
| language | spa |
| publishDate | 2019 |
| publisher | Instituto de Ecología, A.C. |
| record_format | ojs |
| spelling | azm-article-20662022-11-11T21:58:02Z Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos García-Feria, Luis Manuel Brousset, Dulce María Cervantes-Olivares, Roberto Arnulfo Estacionalidad PCR anidada renacuajos índice de vida acuática modelo de distribución de especies CART Seasonality nested PCR tadpoles aquatic life index Specie Distribution Model CART We evaluated the presence of Batrachochytrium dendrobatidis (Bd) in 13 Mexican amphibians associated to abiotic and biotic factors as a species distribution model by a classification and regression tree (CART). Chytrid fungus was diagnosed from cutaneous swabs in adults’ amphibians and oral swabs of tadpoles by nested PCR for a 300bp rRNA fragment. Specimens were sampled in seven vegetation types, during dry and rainy seasons. The chytrid fungus was detected in Charadrayla taeniopus and Leptodactylus fragilis, species that were not previously recorded with the fungus; the Bd also was confirmed on amphibians that inhabit from perennial rainforest at southeastern Mexico. According to CARTs, host species and precipitation were the most important factor to Bd occurrence but their combination with other variables as development stage, environmental temperature, season, vegetation type and aquatic life index have influence on its presence. In addition, the tadpoles had 1.52 times more prevalence of Bd than adults, and that tadpoles from two amphibian species studied could be reservoirs potentially. Although no amphibian showed clinical signs of chytridiomycosis, our findings indicate that the presence and persistence of Bd may be related with species susceptibility or resistance and interaction of diverse abiotic and biotic factors. Evaluamos la presencia de Batrachochytrium dendrobatidis (Bd) en 13 especies de anfibios mexicanos asociada a factores abióticos y bióticos como un modelo de distribución de especies mediante árboles de clasificación y regresión (CART). El hongo quítrido fue diagnosticado a partir de hisopados cutáneos de anfibios adultos, y frotis orales de renacuajos por medio de PCR anidada para un fragmento de rRNA de 300 pb. Los especímenes fueron muestreados en siete tipos de vegetación durante las temporadas seca y lluviosa. El hongo quítrido se detectó por primera vez en Charadrayla taeniopus y Leptodactylus fragilis, especies en las que no se había registrado previamente; también se confirmó su presencia en anfibios de la selva alta perennifolia del sureste de México. De acuerdo con los CART, las dos variables más importantes para la ocurrencia de Bd son la especie del hospedero y la precipitación, pero su combinación con otras variables como el estado de desarrollo, la temperatura ambiental, la temporada climática, el tipo de vegetación y altos índices de vida acuática influyen en la presencia del hongo quítrido. Además, se obtuvo que los renacuajos tienen 1.52 veces más prevalencia de Bd que los adultos, y los renacuajos de dos especies de anfibios estudiadas son potencialmente reservorios. Aunque ningún anfibio mostró signos clínicos de quitridiomicosis, nuestros hallazgos indican que la presencia y la persistencia de Bd pueden estar relacionadas con la susceptibilidad o resistencia de las especies y la interacción de diversos factores abióticos y bióticos. Instituto de Ecología, A.C. 2019-07-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original articles Artículos originales application/pdf application/xml https://azm.ojs.inecol.mx/index.php/azm/article/view/2066 10.21829/azm.2019.3502066 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 35 (2019); 1-18 ACTA ZOOLÓGICA MEXICANA (N.S.); Vol. 35 (2019); 1-18 2448-8445 0065-1737 spa https://azm.ojs.inecol.mx/index.php/azm/article/view/2066/2298 https://azm.ojs.inecol.mx/index.php/azm/article/view/2066/2434 Derechos de autor 2019 ACTA ZOOLÓGICA MEXICANA (N.S.) http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | García-Feria, Luis Manuel Brousset, Dulce María Cervantes-Olivares, Roberto Arnulfo Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos |
| title | Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos |
| title_full | Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos |
| title_fullStr | Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos |
| title_full_unstemmed | Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos |
| title_short | Factores abióticos y bióticos determinantes para la presencia de Batrachochytrium dendrobatidis en anfibios mexicanos |
| title_sort | factores abióticos y bióticos determinantes para la presencia de batrachochytrium dendrobatidis en anfibios mexicanos |
| url | https://azm.ojs.inecol.mx/index.php/azm/article/view/2066 |
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